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GM1 and NGF modulate Ca2+ homeostasis and GAP43 mRNA expression in cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate.
Nutr Neurosci. 2007 Jun-Aug; 10(3-4):105-11.NN

Abstract

Monosialoganglioside (GM1) has been considered to have a neurotrophic factor-like activity. Nerve growth factor (NGF), a member of the neurotrophin family, is essential for neuronal survival, differentiation and maturation. The aim of the present study was to investigate whether co-administration of GM1 and NGF reverses glutamate (Glu) neurotoxicity in primary cultured rat embryonic dorsal root ganglion (DRG) neurons. DRG neurons were exposed to Glu (2 mmol/1), Glu (2 mmol/1) plus GM1 (10 microg/ml), Glu (2 mmol/l) plus NGF (10 ng/ml), Glu (2 mmol/l) plus GM1 (5 microg/ml) and NGF (5 ng/ml) and then processed for detecting intracellular concentrations of Ca2+ ([Ca2+] i) by confocal laser scanning microscopy and growth-associated protein 43 (GAP43) mRNA by RT-PCR. The fluorescent intensity in Glu plus GM1 and NGF incubated neurons was the lowest as compared with that in other groups. The expression of GAP43 mRNA in Glu plus GM1 and NGF incubated neurons was the highest as compared with that in other groups. These results implicated that GM1 and NGF have synergistic neuroprotective effects on DRG neurons with excitotoxicity induced by Glu in vitro.

Authors+Show Affiliations

Department of Anatomy, Shandong University School of Medicine, Jinan 250012, People's Republic of ChinaNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18019391

Citation

Huang, Fei, et al. "GM1 and NGF Modulate Ca2+ Homeostasis and GAP43 mRNA Expression in Cultured Dorsal Root Ganglion Neurons With Excitotoxicity Induced By Glutamate." Nutritional Neuroscience, vol. 10, no. 3-4, 2007, pp. 105-11.
Huang F, Liu Z, Liu H, et al. GM1 and NGF modulate Ca2+ homeostasis and GAP43 mRNA expression in cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate. Nutr Neurosci. 2007;10(3-4):105-11.
Huang, F., Liu, Z., Liu, H., Wang, L., Wang, H., & Li, Z. (2007). GM1 and NGF modulate Ca2+ homeostasis and GAP43 mRNA expression in cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate. Nutritional Neuroscience, 10(3-4), 105-11.
Huang F, et al. GM1 and NGF Modulate Ca2+ Homeostasis and GAP43 mRNA Expression in Cultured Dorsal Root Ganglion Neurons With Excitotoxicity Induced By Glutamate. Nutr Neurosci. 2007 Jun-Aug;10(3-4):105-11. PubMed PMID: 18019391.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GM1 and NGF modulate Ca2+ homeostasis and GAP43 mRNA expression in cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate. AU - Huang,Fei, AU - Liu,Zhen, AU - Liu,Huaxiang, AU - Wang,Lihong, AU - Wang,Huaijing, AU - Li,Zhenzhong, PY - 2007/11/21/pubmed PY - 2008/1/19/medline PY - 2007/11/21/entrez SP - 105 EP - 11 JF - Nutritional neuroscience JO - Nutr Neurosci VL - 10 IS - 3-4 N2 - Monosialoganglioside (GM1) has been considered to have a neurotrophic factor-like activity. Nerve growth factor (NGF), a member of the neurotrophin family, is essential for neuronal survival, differentiation and maturation. The aim of the present study was to investigate whether co-administration of GM1 and NGF reverses glutamate (Glu) neurotoxicity in primary cultured rat embryonic dorsal root ganglion (DRG) neurons. DRG neurons were exposed to Glu (2 mmol/1), Glu (2 mmol/1) plus GM1 (10 microg/ml), Glu (2 mmol/l) plus NGF (10 ng/ml), Glu (2 mmol/l) plus GM1 (5 microg/ml) and NGF (5 ng/ml) and then processed for detecting intracellular concentrations of Ca2+ ([Ca2+] i) by confocal laser scanning microscopy and growth-associated protein 43 (GAP43) mRNA by RT-PCR. The fluorescent intensity in Glu plus GM1 and NGF incubated neurons was the lowest as compared with that in other groups. The expression of GAP43 mRNA in Glu plus GM1 and NGF incubated neurons was the highest as compared with that in other groups. These results implicated that GM1 and NGF have synergistic neuroprotective effects on DRG neurons with excitotoxicity induced by Glu in vitro. SN - 1028-415X UR - https://www.unboundmedicine.com/medline/citation/18019391/GM1_and_NGF_modulate_Ca2+_homeostasis_and_GAP43_mRNA_expression_in_cultured_dorsal_root_ganglion_neurons_with_excitotoxicity_induced_by_glutamate_ L2 - http://www.tandfonline.com/doi/full/10.1080/10284150701406752 DB - PRIME DP - Unbound Medicine ER -