Tags

Type your tag names separated by a space and hit enter

The influence of immuosuppressive therapy on the development of CD4+CD25+ T cells after renal transplantation.
Transplant Proc. 2007 Nov; 39(9):2721-3.TP

Abstract

A growing number of studies suggest that CD4(+)CD25(+) T regulatory (Treg) cells play a significant role to downregulate the immune response to alloantigens. In this study, we investigated the possible influence of immunosuppressive therapy, including cyclosporine (CsA) or rapamycin (sirolimus), on the level of CD4(+)CD25(+), CD4(+)CD25(+)FOXP3(+), and CD4(+)CD25(+)CTLA-4(+) T cells in the peripheral blood of renal allograft recipients. The study was performed on renal allograft recipients who displayed uneventful stable courses (RAR-S; n = 15) versus biopsy-proven chronic rejection (RAR-CH; n = 12). The patients were divided based on the immunosuppressive protocol: group 1 (prednisone+CsA+Aza) and group II (prednisone+sirolimus). The control group consisted of 10 healthy blood donors. We examined the expression of CD4, CD25, CTLA-4, and Foxp3 in peripheral blood T cells. Flow cytometry was performed with a FACSCalibur (BD Biosciences) instrument with data analyzed using Cell Quest software. The percentage of CD4(+)CD25(+)Foxp3(+) T cells in rapamycin (sirolimus) treated patients did not differ from that observed in healthy individuals, but was significantly higher compared with CsA-treated patients. CsA therapy resulted in a reduction in the percentage of CD4(+)CD25(+)CTLA-4(+) and CD4(+)CD25(+)Foxp3(+) regulatory T cells after renal transplantation in both groups (RAR-S and RAR-CH) compared with patients treated with rapamycin or to healthy donors. The type of immunosuppressive therapy (with or without calcineurin inhibitors) may have an important role in tolerance induction and graft function.

Authors+Show Affiliations

Transplantation Institute, Medical University of Warsaw, Warsaw, Poland. gkorczak-k@biol.uw.edu.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18021968

Citation

Korczak-Kowalska, G, et al. "The Influence of Immuosuppressive Therapy On the Development of CD4+CD25+ T Cells After Renal Transplantation." Transplantation Proceedings, vol. 39, no. 9, 2007, pp. 2721-3.
Korczak-Kowalska G, Wierzbicki P, Bocian K, et al. The influence of immuosuppressive therapy on the development of CD4+CD25+ T cells after renal transplantation. Transplant Proc. 2007;39(9):2721-3.
Korczak-Kowalska, G., Wierzbicki, P., Bocian, K., Klosowska, D., Niemczyk, M., Wyzgal, J., Korecka, A., Durlik, M., Chmura, A., Paczek, L., & Górski, A. (2007). The influence of immuosuppressive therapy on the development of CD4+CD25+ T cells after renal transplantation. Transplantation Proceedings, 39(9), 2721-3.
Korczak-Kowalska G, et al. The Influence of Immuosuppressive Therapy On the Development of CD4+CD25+ T Cells After Renal Transplantation. Transplant Proc. 2007;39(9):2721-3. PubMed PMID: 18021968.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The influence of immuosuppressive therapy on the development of CD4+CD25+ T cells after renal transplantation. AU - Korczak-Kowalska,G, AU - Wierzbicki,P, AU - Bocian,K, AU - Klosowska,D, AU - Niemczyk,M, AU - Wyzgal,J, AU - Korecka,A, AU - Durlik,M, AU - Chmura,A, AU - Paczek,L, AU - Górski,A, PY - 2007/11/21/pubmed PY - 2008/1/9/medline PY - 2007/11/21/entrez SP - 2721 EP - 3 JF - Transplantation proceedings JO - Transplant. Proc. VL - 39 IS - 9 N2 - A growing number of studies suggest that CD4(+)CD25(+) T regulatory (Treg) cells play a significant role to downregulate the immune response to alloantigens. In this study, we investigated the possible influence of immunosuppressive therapy, including cyclosporine (CsA) or rapamycin (sirolimus), on the level of CD4(+)CD25(+), CD4(+)CD25(+)FOXP3(+), and CD4(+)CD25(+)CTLA-4(+) T cells in the peripheral blood of renal allograft recipients. The study was performed on renal allograft recipients who displayed uneventful stable courses (RAR-S; n = 15) versus biopsy-proven chronic rejection (RAR-CH; n = 12). The patients were divided based on the immunosuppressive protocol: group 1 (prednisone+CsA+Aza) and group II (prednisone+sirolimus). The control group consisted of 10 healthy blood donors. We examined the expression of CD4, CD25, CTLA-4, and Foxp3 in peripheral blood T cells. Flow cytometry was performed with a FACSCalibur (BD Biosciences) instrument with data analyzed using Cell Quest software. The percentage of CD4(+)CD25(+)Foxp3(+) T cells in rapamycin (sirolimus) treated patients did not differ from that observed in healthy individuals, but was significantly higher compared with CsA-treated patients. CsA therapy resulted in a reduction in the percentage of CD4(+)CD25(+)CTLA-4(+) and CD4(+)CD25(+)Foxp3(+) regulatory T cells after renal transplantation in both groups (RAR-S and RAR-CH) compared with patients treated with rapamycin or to healthy donors. The type of immunosuppressive therapy (with or without calcineurin inhibitors) may have an important role in tolerance induction and graft function. SN - 0041-1345 UR - https://www.unboundmedicine.com/medline/citation/18021968/The_influence_of_immuosuppressive_therapy_on_the_development_of_CD4+CD25+_T_cells_after_renal_transplantation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-1345(07)01165-7 DB - PRIME DP - Unbound Medicine ER -