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Development of "Pinceaux" formations and dendritic translocation of climbing fibers during the acquisition of the balance between glutamatergic and gamma-aminobutyric acidergic inputs in developing Purkinje cells.
J Comp Neurol 2008; 506(2):240-62JC

Abstract

The acquisition of the dynamic balance between excitation and inhibition in developing Purkinje cells, necessary for their proper function, is analyzed. Newborn (P0) mouse cerebellum contains glutamatergic (VGLUT2-IR) and gamma-aminobutyric acid (GABA)-ergic (VIAAT-IR) axons. The former prevail and belong to climbing fibers, whereas the latter neither colabel with calbindin-expressing fibers nor belong to axons of the cortical GABAergic interneurons. During the first postnatal week, VIAAT-IR axons in the Purkinje cell neighborhood remains very low, and the first synapses with basket fibers are formed at P7, when climbing fibers have already established dense pericellular nets. The descending basket fibers reach the Purkinje cell axon initial segment by P9, immediately establishing axoaxonic synapses. The pinceaux appear as primitive vortex-like arrangements by P12, and by P20 interbasket fiber septate-like junctions, typical of fully mature pinceaux, are still missing. The climbing fiber's somatodendritic translocation occurs later than expected, after the regression of the multiple innervation, and follows the ascending collaterals of the basket axons, which are apparently the optimal substrate for the proper subcellular targeting of the climbing fibers. These results emphasize that chemical transmission in the axon initial segment precedes the electrical inhibition generated by field effects. In addition, GABAergic Purkinje cells, as opposed to glutamatergic projection neurons in other cortical structures, do not begin to receive their excitation to inhibition balance until the end of the first postnatal week, despite the early presence of potentially functional GABAergic axons that possess the required vesicular transport system.

Authors+Show Affiliations

Cátedra de Neurobiología del Desarrollo "Remedios Caro Almela," Instituto de Neurociencias de Alicante, Universidad Miguel Hernández y CSIC, Sant Joan d'Alacant, 03550 Alicante, Spain. sotelo@umh.es

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18022955

Citation

Sotelo, Constantino. "Development of "Pinceaux" Formations and Dendritic Translocation of Climbing Fibers During the Acquisition of the Balance Between Glutamatergic and Gamma-aminobutyric Acidergic Inputs in Developing Purkinje Cells." The Journal of Comparative Neurology, vol. 506, no. 2, 2008, pp. 240-62.
Sotelo C. Development of "Pinceaux" formations and dendritic translocation of climbing fibers during the acquisition of the balance between glutamatergic and gamma-aminobutyric acidergic inputs in developing Purkinje cells. J Comp Neurol. 2008;506(2):240-62.
Sotelo, C. (2008). Development of "Pinceaux" formations and dendritic translocation of climbing fibers during the acquisition of the balance between glutamatergic and gamma-aminobutyric acidergic inputs in developing Purkinje cells. The Journal of Comparative Neurology, 506(2), pp. 240-62.
Sotelo C. Development of "Pinceaux" Formations and Dendritic Translocation of Climbing Fibers During the Acquisition of the Balance Between Glutamatergic and Gamma-aminobutyric Acidergic Inputs in Developing Purkinje Cells. J Comp Neurol. 2008 Jan 10;506(2):240-62. PubMed PMID: 18022955.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of "Pinceaux" formations and dendritic translocation of climbing fibers during the acquisition of the balance between glutamatergic and gamma-aminobutyric acidergic inputs in developing Purkinje cells. A1 - Sotelo,Constantino, PY - 2007/11/21/pubmed PY - 2008/3/1/medline PY - 2007/11/21/entrez SP - 240 EP - 62 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 506 IS - 2 N2 - The acquisition of the dynamic balance between excitation and inhibition in developing Purkinje cells, necessary for their proper function, is analyzed. Newborn (P0) mouse cerebellum contains glutamatergic (VGLUT2-IR) and gamma-aminobutyric acid (GABA)-ergic (VIAAT-IR) axons. The former prevail and belong to climbing fibers, whereas the latter neither colabel with calbindin-expressing fibers nor belong to axons of the cortical GABAergic interneurons. During the first postnatal week, VIAAT-IR axons in the Purkinje cell neighborhood remains very low, and the first synapses with basket fibers are formed at P7, when climbing fibers have already established dense pericellular nets. The descending basket fibers reach the Purkinje cell axon initial segment by P9, immediately establishing axoaxonic synapses. The pinceaux appear as primitive vortex-like arrangements by P12, and by P20 interbasket fiber septate-like junctions, typical of fully mature pinceaux, are still missing. The climbing fiber's somatodendritic translocation occurs later than expected, after the regression of the multiple innervation, and follows the ascending collaterals of the basket axons, which are apparently the optimal substrate for the proper subcellular targeting of the climbing fibers. These results emphasize that chemical transmission in the axon initial segment precedes the electrical inhibition generated by field effects. In addition, GABAergic Purkinje cells, as opposed to glutamatergic projection neurons in other cortical structures, do not begin to receive their excitation to inhibition balance until the end of the first postnatal week, despite the early presence of potentially functional GABAergic axons that possess the required vesicular transport system. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/18022955/Development_of_"Pinceaux"_formations_and_dendritic_translocation_of_climbing_fibers_during_the_acquisition_of_the_balance_between_glutamatergic_and_gamma_aminobutyric_acidergic_inputs_in_developing_Purkinje_cells_ L2 - https://doi.org/10.1002/cne.21501 DB - PRIME DP - Unbound Medicine ER -