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Expressions of natural cytotoxicity receptors and NKG2D on decidual natural killer cells in patients having spontaneous abortions.

Abstract

OBJECTIVE

To investigate the relation between expressions of NKp46, NKp44, NKp30, and NKG2D receptors on decidual natural killer cells (dNK) and human spontaneous abortion (SA).

DESIGN

Case control study.

SETTING

University hospital.

PATIENT(S)

Twenty-one patients with normal chromosome abortuses and 25 healthy early pregnant women.

INTERVENTION(S)

Detected the cytolytic activity of dNK to K562 target cells; measured the expressions of NCRs and NKG2D on dNK.

MAIN OUTCOME MEASURE(S)

Cytotoxicity of dNK and expressions of NKp46, p44, p30, and NKG2D receptors on CD56(bright)CD16(-)/CD56(dim)CD16(+) dNK subsets were detected by flow cytometry. The relations between dNK cytotoxicity and the expression of the receptors on two dNK subsets were investigated.

RESULT(S)

The patients with SA expressed higher proportion of NKp44 on CD56(bright)CD16(-)dNK and higher proportions of NKp46 and NKp44 on CD56(dim)CD16(+)dNK with statistical significance. The expressions of NKp44 on CD56(bright)CD16(-)dNK and NKp46 on CD56(dim)CD16(+)dNK correlated with dNK cytotoxicity positively (r(2) = 0.677 and r(2) = 0.634, respectively). The expression of NKp46 on CD56(bright)CD16(-)dNK was much higher than that on CD56(dim)CD16(+)dNK.

CONCLUSION(S)

Enhanced dNK cytotoxicity due to increased NKp46 and NKp44 expressions may undergo the susceptibility to SA. Both CD56(bright)CD16(-)dNK and CD56(dim)CD16(+) dNK may be involved in lysing of the target cells.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.

    , , , ,

    Source

    Fertility and sterility 90:5 2008 Nov pg 1931-7

    MeSH

    Abortion, Spontaneous
    Adult
    CD56 Antigen
    Case-Control Studies
    Cell Separation
    Cell Survival
    Coculture Techniques
    Cytotoxicity, Immunologic
    Decidua
    Female
    Flow Cytometry
    GPI-Linked Proteins
    Humans
    K562 Cells
    Killer Cells, Natural
    NK Cell Lectin-Like Receptor Subfamily K
    Natural Cytotoxicity Triggering Receptor 1
    Natural Cytotoxicity Triggering Receptor 2
    Natural Cytotoxicity Triggering Receptor 3
    Receptors, IgG
    Receptors, Natural Cytotoxicity Triggering
    Young Adult

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    18023431

    Citation

    Zhang, Yu, et al. "Expressions of Natural Cytotoxicity Receptors and NKG2D On Decidual Natural Killer Cells in Patients Having Spontaneous Abortions." Fertility and Sterility, vol. 90, no. 5, 2008, pp. 1931-7.
    Zhang Y, Zhao A, Wang X, et al. Expressions of natural cytotoxicity receptors and NKG2D on decidual natural killer cells in patients having spontaneous abortions. Fertil Steril. 2008;90(5):1931-7.
    Zhang, Y., Zhao, A., Wang, X., Shi, G., Jin, H., & Lin, Q. (2008). Expressions of natural cytotoxicity receptors and NKG2D on decidual natural killer cells in patients having spontaneous abortions. Fertility and Sterility, 90(5), pp. 1931-7.
    Zhang Y, et al. Expressions of Natural Cytotoxicity Receptors and NKG2D On Decidual Natural Killer Cells in Patients Having Spontaneous Abortions. Fertil Steril. 2008;90(5):1931-7. PubMed PMID: 18023431.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Expressions of natural cytotoxicity receptors and NKG2D on decidual natural killer cells in patients having spontaneous abortions. AU - Zhang,Yu, AU - Zhao,Aimin, AU - Wang,Xipeng, AU - Shi,Guiying, AU - Jin,Huifang, AU - Lin,Qide, Y1 - 2007/11/26/ PY - 2007/07/25/received PY - 2007/08/06/revised PY - 2007/08/06/accepted PY - 2007/11/21/pubmed PY - 2008/12/17/medline PY - 2007/11/21/entrez SP - 1931 EP - 7 JF - Fertility and sterility JO - Fertil. Steril. VL - 90 IS - 5 N2 - OBJECTIVE: To investigate the relation between expressions of NKp46, NKp44, NKp30, and NKG2D receptors on decidual natural killer cells (dNK) and human spontaneous abortion (SA). DESIGN: Case control study. SETTING: University hospital. PATIENT(S): Twenty-one patients with normal chromosome abortuses and 25 healthy early pregnant women. INTERVENTION(S): Detected the cytolytic activity of dNK to K562 target cells; measured the expressions of NCRs and NKG2D on dNK. MAIN OUTCOME MEASURE(S): Cytotoxicity of dNK and expressions of NKp46, p44, p30, and NKG2D receptors on CD56(bright)CD16(-)/CD56(dim)CD16(+) dNK subsets were detected by flow cytometry. The relations between dNK cytotoxicity and the expression of the receptors on two dNK subsets were investigated. RESULT(S): The patients with SA expressed higher proportion of NKp44 on CD56(bright)CD16(-)dNK and higher proportions of NKp46 and NKp44 on CD56(dim)CD16(+)dNK with statistical significance. The expressions of NKp44 on CD56(bright)CD16(-)dNK and NKp46 on CD56(dim)CD16(+)dNK correlated with dNK cytotoxicity positively (r(2) = 0.677 and r(2) = 0.634, respectively). The expression of NKp46 on CD56(bright)CD16(-)dNK was much higher than that on CD56(dim)CD16(+)dNK. CONCLUSION(S): Enhanced dNK cytotoxicity due to increased NKp46 and NKp44 expressions may undergo the susceptibility to SA. Both CD56(bright)CD16(-)dNK and CD56(dim)CD16(+) dNK may be involved in lysing of the target cells. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/18023431/Expressions_of_natural_cytotoxicity_receptors_and_NKG2D_on_decidual_natural_killer_cells_in_patients_having_spontaneous_abortions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(07)03152-4 DB - PRIME DP - Unbound Medicine ER -