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Evaluation of genotoxic and antigenotoxic effects of hydroalcoholic extracts of Zuccagnia punctata Cav.
J Ethnopharmacol. 2008 Jan 17; 115(2):330-5.JE

Abstract

Zuccagnia punctata Cav. (Fabaceae), a widely used plant species in Argentine folk medicine, has been shown to have a broad spectrum of antibacterial, antifungal, antioxidant and cytoprotective activities. In this study, the hydroalcoholic extract of Zuccagnia punctata and 2',4'-dihydroxychalcone isolated from it were investigated for genotoxicity/antigenotoxicity in the in vitro comet assay test on human hepatoma HepG2 cells. No acute toxicity of the extract could be determined. HepG2 cells were treated with three different concentrations (2.5, 5.0 and 10.0 microg/mL) or 2',4'-dihydroxychalcone (0.01, 0.10 and 1.00 microg/mL). To explore the potential mechanisms of action, two approaches were followed: co-treatment with 4-nitroquinoline-N-oxyde (4-NQO), a direct genotoxic compound, and a pre-treatment protocol with benzo[a]pyrene (B[a]P), an indirect genotoxic compound. The natural products neither affected cell viability nor induced DNA damage in the concentration range tested. Zuccagnia punctata tinctures were able to diminish the DNA damage induced in HepG2 cells by 4-NQO and B[a]P in 31% and 10%, respectively at 10 microg/mL. Pre-treatment of HepG2 cells with 2',4'-dihydroxychalcone was highly effective in decreasing B[a]P-induced DNA damage at a statistically significant level, with an almost clear dose-response relationship. The inhibition values were 28.2-43.9% for the tested concentrations of 0.01-1 microg/mL, respectively. The results clearly indicate that the phytoextract from Zuccagnia punctata, under the experimental conditions tested, is not genotoxic and that 2',4'-dihydroxychalcone contributes to a high degree to the antigenotoxic effects of Zuccagnia punctata tincture.

Authors+Show Affiliations

Cátedra de Fitoquímica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho 471, 4000 San Miguel de Tucumán, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18023546

Citation

Zampini, Iris Catiana, et al. "Evaluation of Genotoxic and Antigenotoxic Effects of Hydroalcoholic Extracts of Zuccagnia Punctata Cav." Journal of Ethnopharmacology, vol. 115, no. 2, 2008, pp. 330-5.
Zampini IC, Villarini M, Moretti M, et al. Evaluation of genotoxic and antigenotoxic effects of hydroalcoholic extracts of Zuccagnia punctata Cav. J Ethnopharmacol. 2008;115(2):330-5.
Zampini, I. C., Villarini, M., Moretti, M., Dominici, L., & Isla, M. I. (2008). Evaluation of genotoxic and antigenotoxic effects of hydroalcoholic extracts of Zuccagnia punctata Cav. Journal of Ethnopharmacology, 115(2), 330-5.
Zampini IC, et al. Evaluation of Genotoxic and Antigenotoxic Effects of Hydroalcoholic Extracts of Zuccagnia Punctata Cav. J Ethnopharmacol. 2008 Jan 17;115(2):330-5. PubMed PMID: 18023546.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of genotoxic and antigenotoxic effects of hydroalcoholic extracts of Zuccagnia punctata Cav. AU - Zampini,Iris Catiana, AU - Villarini,Milena, AU - Moretti,Massimo, AU - Dominici,Luca, AU - Isla,María Inés, Y1 - 2007/10/12/ PY - 2007/08/24/received PY - 2007/09/29/revised PY - 2007/10/06/accepted PY - 2007/11/21/pubmed PY - 2008/4/23/medline PY - 2007/11/21/entrez SP - 330 EP - 5 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 115 IS - 2 N2 - Zuccagnia punctata Cav. (Fabaceae), a widely used plant species in Argentine folk medicine, has been shown to have a broad spectrum of antibacterial, antifungal, antioxidant and cytoprotective activities. In this study, the hydroalcoholic extract of Zuccagnia punctata and 2',4'-dihydroxychalcone isolated from it were investigated for genotoxicity/antigenotoxicity in the in vitro comet assay test on human hepatoma HepG2 cells. No acute toxicity of the extract could be determined. HepG2 cells were treated with three different concentrations (2.5, 5.0 and 10.0 microg/mL) or 2',4'-dihydroxychalcone (0.01, 0.10 and 1.00 microg/mL). To explore the potential mechanisms of action, two approaches were followed: co-treatment with 4-nitroquinoline-N-oxyde (4-NQO), a direct genotoxic compound, and a pre-treatment protocol with benzo[a]pyrene (B[a]P), an indirect genotoxic compound. The natural products neither affected cell viability nor induced DNA damage in the concentration range tested. Zuccagnia punctata tinctures were able to diminish the DNA damage induced in HepG2 cells by 4-NQO and B[a]P in 31% and 10%, respectively at 10 microg/mL. Pre-treatment of HepG2 cells with 2',4'-dihydroxychalcone was highly effective in decreasing B[a]P-induced DNA damage at a statistically significant level, with an almost clear dose-response relationship. The inhibition values were 28.2-43.9% for the tested concentrations of 0.01-1 microg/mL, respectively. The results clearly indicate that the phytoextract from Zuccagnia punctata, under the experimental conditions tested, is not genotoxic and that 2',4'-dihydroxychalcone contributes to a high degree to the antigenotoxic effects of Zuccagnia punctata tincture. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/18023546/Evaluation_of_genotoxic_and_antigenotoxic_effects_of_hydroalcoholic_extracts_of_Zuccagnia_punctata_Cav_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(07)00534-X DB - PRIME DP - Unbound Medicine ER -