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PLGA, chitosan or chitosan-coated PLGA microparticles for alveolar delivery? A comparative study of particle stability during nebulization.
Colloids Surf B Biointerfaces 2008; 62(2):220-31CS

Abstract

Various types of rifampicin (RIF)-loaded microparticles were compared for their stability during nebulization. Poly(lactide-co-glycolide) (PLGA), chitosan (CHT) and PLGA/CHT microparticles (MPs) were prepared by emulsion or precipitation techniques. MPs ability to be nebulized (NE%) as well as stability during freeze-drying or/and nebulization (NEED%), were evaluated after RIF extraction from MPs and determination by light spectroscopy. MP mean diameters and zeta-potential values were measured by dynamic light scattering, morphology was assessed by SEM, cytotoxicity by MTT method and mucoadhesive properties by mucin association. In all cases, freeze-drying prior to nebulization did not affect EE%, NE or NEED%. In CHT, MPs RIF encapsulation efficiency (EE%) decreased with increasing CHT concentration (viscosity) and CHT-MP NEED% was higher when the polymer was crosslinked by glutaraldehyde. PLGA MPs, exhibited both higher RIF EE% and also higher nebulization ability and NEED%, compared to CHT ones, but also higher cytotoxicity. However, when the two polymers were combined in the PLGA/CHT MPs, EE%, NE% and NEED% increased with increasing MP CHT-content. PLGA/CHT MPs with 0.50% or 0.75% CHT exhibited highest EE% for RIF and also best nebulization ability and stability, compared to all other MP formulations studied. Additionally they had good mucoadhesive properties and comparably low cytotoxicity.

Authors+Show Affiliations

Laboratory of Pharmaceutical Technology, Department of Pharmacy, University of Patras, Rio 26510, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18023977

Citation

Manca, Maria-Letizia, et al. "PLGA, Chitosan or Chitosan-coated PLGA Microparticles for Alveolar Delivery? a Comparative Study of Particle Stability During Nebulization." Colloids and Surfaces. B, Biointerfaces, vol. 62, no. 2, 2008, pp. 220-31.
Manca ML, Mourtas S, Dracopoulos V, et al. PLGA, chitosan or chitosan-coated PLGA microparticles for alveolar delivery? A comparative study of particle stability during nebulization. Colloids Surf B Biointerfaces. 2008;62(2):220-31.
Manca, M. L., Mourtas, S., Dracopoulos, V., Fadda, A. M., & Antimisiaris, S. G. (2008). PLGA, chitosan or chitosan-coated PLGA microparticles for alveolar delivery? A comparative study of particle stability during nebulization. Colloids and Surfaces. B, Biointerfaces, 62(2), pp. 220-31.
Manca ML, et al. PLGA, Chitosan or Chitosan-coated PLGA Microparticles for Alveolar Delivery? a Comparative Study of Particle Stability During Nebulization. Colloids Surf B Biointerfaces. 2008 Apr 1;62(2):220-31. PubMed PMID: 18023977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PLGA, chitosan or chitosan-coated PLGA microparticles for alveolar delivery? A comparative study of particle stability during nebulization. AU - Manca,Maria-Letizia, AU - Mourtas,Spyridon, AU - Dracopoulos,Vassileios, AU - Fadda,Anna Maria, AU - Antimisiaris,Sophia G, Y1 - 2007/10/11/ PY - 2007/07/31/received PY - 2007/10/01/revised PY - 2007/10/06/accepted PY - 2007/11/21/pubmed PY - 2008/5/14/medline PY - 2007/11/21/entrez SP - 220 EP - 31 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 62 IS - 2 N2 - Various types of rifampicin (RIF)-loaded microparticles were compared for their stability during nebulization. Poly(lactide-co-glycolide) (PLGA), chitosan (CHT) and PLGA/CHT microparticles (MPs) were prepared by emulsion or precipitation techniques. MPs ability to be nebulized (NE%) as well as stability during freeze-drying or/and nebulization (NEED%), were evaluated after RIF extraction from MPs and determination by light spectroscopy. MP mean diameters and zeta-potential values were measured by dynamic light scattering, morphology was assessed by SEM, cytotoxicity by MTT method and mucoadhesive properties by mucin association. In all cases, freeze-drying prior to nebulization did not affect EE%, NE or NEED%. In CHT, MPs RIF encapsulation efficiency (EE%) decreased with increasing CHT concentration (viscosity) and CHT-MP NEED% was higher when the polymer was crosslinked by glutaraldehyde. PLGA MPs, exhibited both higher RIF EE% and also higher nebulization ability and NEED%, compared to CHT ones, but also higher cytotoxicity. However, when the two polymers were combined in the PLGA/CHT MPs, EE%, NE% and NEED% increased with increasing MP CHT-content. PLGA/CHT MPs with 0.50% or 0.75% CHT exhibited highest EE% for RIF and also best nebulization ability and stability, compared to all other MP formulations studied. Additionally they had good mucoadhesive properties and comparably low cytotoxicity. SN - 0927-7765 UR - https://www.unboundmedicine.com/medline/citation/18023977/PLGA_chitosan_or_chitosan_coated_PLGA_microparticles_for_alveolar_delivery_A_comparative_study_of_particle_stability_during_nebulization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(07)00397-9 DB - PRIME DP - Unbound Medicine ER -