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Dietary nitrite supplementation protects against myocardial ischemia-reperfusion injury.
Proc Natl Acad Sci U S A 2007; 104(48):19144-9PN

Abstract

Nitrite has emerged as an endogenous signaling molecule with potential therapeutic implications for cardiovascular disease. Steady-state levels of nitrite are derived in part from dietary sources; therefore, we investigated the effects of dietary nitrite and nitrate supplementation and deficiency on NO homeostasis and on the severity of myocardial ischemia-reperfusion (MI/R) injury. Mice fed a standard diet with supplementation of nitrite (50 mg/liter) in their drinking water for 7 days exhibited significantly higher plasma levels of nitrite, exhibited significantly higher myocardial levels of nitrite, nitroso, and nitrosyl-heme, and displayed a 48% reduction in infarct size (Inf) after MI/R. Supplemental nitrate (1 g/liter) in the drinking water for 7 days also increased blood and tissue NO products and significantly reduced Inf. A time course of ischemia-reperfusion revealed that nitrite was consumed during the ischemic phase, with an increase in nitroso/nitrosyl products in the heart. Mice fed a diet deficient in nitrite and nitrate for 7 days exhibited significantly diminished plasma and heart levels of nitrite and NO metabolites and a 59% increase in Inf after MI/R. Supplementation of nitrite in the drinking water for 7 days reversed the effects of nitrite deficiency. These data demonstrate the significant influence of dietary nitrite and nitrate intake on the maintenance of steady-state tissue nitrite/nitroso levels and illustrate the consequences of nitrite deficiency on the pathophysiology of MI/R injury. Therefore, nitrite and nitrate may serve as essential nutrients for optimal cardiovascular health and may provide a treatment modality for cardiovascular disease.

Authors+Show Affiliations

Institute of Molecular Medicine, University of Texas Health Science Center, Houston, TX 77030, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18025468

Citation

Bryan, Nathan S., et al. "Dietary Nitrite Supplementation Protects Against Myocardial Ischemia-reperfusion Injury." Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 48, 2007, pp. 19144-9.
Bryan NS, Calvert JW, Elrod JW, et al. Dietary nitrite supplementation protects against myocardial ischemia-reperfusion injury. Proc Natl Acad Sci USA. 2007;104(48):19144-9.
Bryan, N. S., Calvert, J. W., Elrod, J. W., Gundewar, S., Ji, S. Y., & Lefer, D. J. (2007). Dietary nitrite supplementation protects against myocardial ischemia-reperfusion injury. Proceedings of the National Academy of Sciences of the United States of America, 104(48), pp. 19144-9.
Bryan NS, et al. Dietary Nitrite Supplementation Protects Against Myocardial Ischemia-reperfusion Injury. Proc Natl Acad Sci USA. 2007 Nov 27;104(48):19144-9. PubMed PMID: 18025468.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary nitrite supplementation protects against myocardial ischemia-reperfusion injury. AU - Bryan,Nathan S, AU - Calvert,John W, AU - Elrod,John W, AU - Gundewar,Susheel, AU - Ji,Sang Yong, AU - Lefer,David J, Y1 - 2007/11/19/ PY - 2007/11/21/pubmed PY - 2008/1/16/medline PY - 2007/11/21/entrez SP - 19144 EP - 9 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 104 IS - 48 N2 - Nitrite has emerged as an endogenous signaling molecule with potential therapeutic implications for cardiovascular disease. Steady-state levels of nitrite are derived in part from dietary sources; therefore, we investigated the effects of dietary nitrite and nitrate supplementation and deficiency on NO homeostasis and on the severity of myocardial ischemia-reperfusion (MI/R) injury. Mice fed a standard diet with supplementation of nitrite (50 mg/liter) in their drinking water for 7 days exhibited significantly higher plasma levels of nitrite, exhibited significantly higher myocardial levels of nitrite, nitroso, and nitrosyl-heme, and displayed a 48% reduction in infarct size (Inf) after MI/R. Supplemental nitrate (1 g/liter) in the drinking water for 7 days also increased blood and tissue NO products and significantly reduced Inf. A time course of ischemia-reperfusion revealed that nitrite was consumed during the ischemic phase, with an increase in nitroso/nitrosyl products in the heart. Mice fed a diet deficient in nitrite and nitrate for 7 days exhibited significantly diminished plasma and heart levels of nitrite and NO metabolites and a 59% increase in Inf after MI/R. Supplementation of nitrite in the drinking water for 7 days reversed the effects of nitrite deficiency. These data demonstrate the significant influence of dietary nitrite and nitrate intake on the maintenance of steady-state tissue nitrite/nitroso levels and illustrate the consequences of nitrite deficiency on the pathophysiology of MI/R injury. Therefore, nitrite and nitrate may serve as essential nutrients for optimal cardiovascular health and may provide a treatment modality for cardiovascular disease. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/18025468/Dietary_nitrite_supplementation_protects_against_myocardial_ischemia_reperfusion_injury_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=18025468 DB - PRIME DP - Unbound Medicine ER -