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Isolation and characterization of genomic retrotransposon sequences from octoploid strawberry (Fragaria x ananassa Duch.).
Plant Cell Rep. 2008 Mar; 27(3):499-507.PC

Abstract

Strawberry (Fragaria spp.) is a kind of herbaceous perennial plant that propagates vegetatively. The conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy groups of LTR retrotransposons were amplified from the cultivated strawberry (Fragaria x ananassa Duch.). Sequence analysis of clones demonstrated that 5 of 19 Ty1-copia group unique sequences and 2 of 10 Ty3-gypsy unique sequences in F. x ananassa genome possessed either stop codon or frameshift. Ty1-copia group sequences are highly heterogeneous (divergence ranged from 1 to 69.8%), but the Ty3-gypsy group sequences are less (divergence ranged from 1 to 10%). Southern dot blot hybridization result suggested that both of the LTR retrotransposons are present in the genome of cultivated strawberry with high copy number (Ty1-copia group 2,875 Ty3-gypsy group 348). RT-PCR amplification from total RNA, which was extracted from leaves of micropropagated strawberry plants, did not yield either of the RT fragments. This is the first report on the presence of RT sequences of Ty1-copia and Ty3-gypsy group retrotransposons in F. x ananassa genome.

Authors+Show Affiliations

College of Horticulture, Shenyang Agricultural University, Shenyang, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18026732

Citation

Ma, Yue, et al. "Isolation and Characterization of Genomic Retrotransposon Sequences From Octoploid Strawberry (Fragaria X Ananassa Duch.)." Plant Cell Reports, vol. 27, no. 3, 2008, pp. 499-507.
Ma Y, Sun H, Zhao G, et al. Isolation and characterization of genomic retrotransposon sequences from octoploid strawberry (Fragaria x ananassa Duch.). Plant Cell Rep. 2008;27(3):499-507.
Ma, Y., Sun, H., Zhao, G., Dai, H., Gao, X., Li, H., & Zhang, Z. (2008). Isolation and characterization of genomic retrotransposon sequences from octoploid strawberry (Fragaria x ananassa Duch.). Plant Cell Reports, 27(3), 499-507.
Ma Y, et al. Isolation and Characterization of Genomic Retrotransposon Sequences From Octoploid Strawberry (Fragaria X Ananassa Duch.). Plant Cell Rep. 2008;27(3):499-507. PubMed PMID: 18026732.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and characterization of genomic retrotransposon sequences from octoploid strawberry (Fragaria x ananassa Duch.). AU - Ma,Yue, AU - Sun,Haiyue, AU - Zhao,Guiling, AU - Dai,Hongyan, AU - Gao,Xiuyan, AU - Li,He, AU - Zhang,Zhihong, Y1 - 2007/11/17/ PY - 2007/08/19/received PY - 2007/10/29/accepted PY - 2007/10/21/revised PY - 2007/11/21/pubmed PY - 2008/7/17/medline PY - 2007/11/21/entrez SP - 499 EP - 507 JF - Plant cell reports JO - Plant Cell Rep VL - 27 IS - 3 N2 - Strawberry (Fragaria spp.) is a kind of herbaceous perennial plant that propagates vegetatively. The conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy groups of LTR retrotransposons were amplified from the cultivated strawberry (Fragaria x ananassa Duch.). Sequence analysis of clones demonstrated that 5 of 19 Ty1-copia group unique sequences and 2 of 10 Ty3-gypsy unique sequences in F. x ananassa genome possessed either stop codon or frameshift. Ty1-copia group sequences are highly heterogeneous (divergence ranged from 1 to 69.8%), but the Ty3-gypsy group sequences are less (divergence ranged from 1 to 10%). Southern dot blot hybridization result suggested that both of the LTR retrotransposons are present in the genome of cultivated strawberry with high copy number (Ty1-copia group 2,875 Ty3-gypsy group 348). RT-PCR amplification from total RNA, which was extracted from leaves of micropropagated strawberry plants, did not yield either of the RT fragments. This is the first report on the presence of RT sequences of Ty1-copia and Ty3-gypsy group retrotransposons in F. x ananassa genome. SN - 0721-7714 UR - https://www.unboundmedicine.com/medline/citation/18026732/Isolation_and_characterization_of_genomic_retrotransposon_sequences_from_octoploid_strawberry__Fragaria_x_ananassa_Duch___ L2 - http://dx.doi.org/10.1007/s00299-007-0476-7 DB - PRIME DP - Unbound Medicine ER -