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Characterization of thyroglobulin epitopes in patients with autoimmune and non-autoimmune thyroid diseases using recombinant human monoclonal thyroglobulin autoantibodies.
J Clin Endocrinol Metab. 2008 Feb; 93(2):591-6.JC

Abstract

CONTEXT

Thyroglobulin (Tg) epitopes of serum Tg autoantibodies (TgAb) have been characterized using inhibition of Tg binding by human monoclonal TgAb in autoimmune thyroid diseases (AITD) [Hashimoto's thyroiditis (HT) and Graves' disease (GD)] but not in non-AITD [nontoxic multinodular goiter (NTMG) and papillary thyroid carcinoma (PTC)].

OBJECTIVE

Our objective was to compare Tg epitopes of serum TgAb from patients with AITD, non-AITD, and PTC associated with histological thyroiditis (PTC-T) using inhibition of Tg binding by four recombinant human TgAb-Fab (epitopic regions A-D).

DESIGN

Inhibition of Tg binding of 24 HT, 25 GD, 19 NTMG, 15 PTC, and 25 PTC-T TgAb-positive sera by each TgAb-Fab was evaluated in ELISA. Inhibition by the pool of the four TgAb-Fab was evaluated using labeled Tg.

RESULTS

Levels of inhibition were different for TgAb-Fab regions A (P = 0.001), B (0.007), and D (0.011). Inhibition by region A TgAb-Fab was significantly higher in HT, GD, and PTC-T than in NTMG and PTC patients. Inhibition levels by region B TgAb-Fab were significantly higher in HT compared with NTMG and PTC patients and in GD compared with NTMG patients. Inhibition by D region TgAb-Fab was significantly lower in NTMG than in the other groups. Inhibition by the pool ranged from 44% (NTMG) to 72% (GD).

CONCLUSIONS

The pattern of Tg recognition is similar when HT patients are compared to GD and NTMG to PTC patients and differs when AITD are compared with non-AITD patients. In PTC-T patients, it is similar to that of AITD patients.

Authors+Show Affiliations

Department of Endocrinology, University Hospital of Pisa, Via Cisanello 2, 56124 Pisa, Italy. latrofaf@libero.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18029466

Citation

Latrofa, Francesco, et al. "Characterization of Thyroglobulin Epitopes in Patients With Autoimmune and Non-autoimmune Thyroid Diseases Using Recombinant Human Monoclonal Thyroglobulin Autoantibodies." The Journal of Clinical Endocrinology and Metabolism, vol. 93, no. 2, 2008, pp. 591-6.
Latrofa F, Ricci D, Grasso L, et al. Characterization of thyroglobulin epitopes in patients with autoimmune and non-autoimmune thyroid diseases using recombinant human monoclonal thyroglobulin autoantibodies. J Clin Endocrinol Metab. 2008;93(2):591-6.
Latrofa, F., Ricci, D., Grasso, L., Vitti, P., Masserini, L., Basolo, F., Ugolini, C., Mascia, G., Lucacchini, A., & Pinchera, A. (2008). Characterization of thyroglobulin epitopes in patients with autoimmune and non-autoimmune thyroid diseases using recombinant human monoclonal thyroglobulin autoantibodies. The Journal of Clinical Endocrinology and Metabolism, 93(2), 591-6.
Latrofa F, et al. Characterization of Thyroglobulin Epitopes in Patients With Autoimmune and Non-autoimmune Thyroid Diseases Using Recombinant Human Monoclonal Thyroglobulin Autoantibodies. J Clin Endocrinol Metab. 2008;93(2):591-6. PubMed PMID: 18029466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of thyroglobulin epitopes in patients with autoimmune and non-autoimmune thyroid diseases using recombinant human monoclonal thyroglobulin autoantibodies. AU - Latrofa,Francesco, AU - Ricci,Debora, AU - Grasso,Lucia, AU - Vitti,Paolo, AU - Masserini,Lucio, AU - Basolo,Fulvio, AU - Ugolini,Clara, AU - Mascia,Giovanni, AU - Lucacchini,Antonio, AU - Pinchera,Aldo, Y1 - 2007/11/20/ PY - 2007/11/22/pubmed PY - 2008/4/11/medline PY - 2007/11/22/entrez SP - 591 EP - 6 JF - The Journal of clinical endocrinology and metabolism JO - J. Clin. Endocrinol. Metab. VL - 93 IS - 2 N2 - CONTEXT: Thyroglobulin (Tg) epitopes of serum Tg autoantibodies (TgAb) have been characterized using inhibition of Tg binding by human monoclonal TgAb in autoimmune thyroid diseases (AITD) [Hashimoto's thyroiditis (HT) and Graves' disease (GD)] but not in non-AITD [nontoxic multinodular goiter (NTMG) and papillary thyroid carcinoma (PTC)]. OBJECTIVE: Our objective was to compare Tg epitopes of serum TgAb from patients with AITD, non-AITD, and PTC associated with histological thyroiditis (PTC-T) using inhibition of Tg binding by four recombinant human TgAb-Fab (epitopic regions A-D). DESIGN: Inhibition of Tg binding of 24 HT, 25 GD, 19 NTMG, 15 PTC, and 25 PTC-T TgAb-positive sera by each TgAb-Fab was evaluated in ELISA. Inhibition by the pool of the four TgAb-Fab was evaluated using labeled Tg. RESULTS: Levels of inhibition were different for TgAb-Fab regions A (P = 0.001), B (0.007), and D (0.011). Inhibition by region A TgAb-Fab was significantly higher in HT, GD, and PTC-T than in NTMG and PTC patients. Inhibition levels by region B TgAb-Fab were significantly higher in HT compared with NTMG and PTC patients and in GD compared with NTMG patients. Inhibition by D region TgAb-Fab was significantly lower in NTMG than in the other groups. Inhibition by the pool ranged from 44% (NTMG) to 72% (GD). CONCLUSIONS: The pattern of Tg recognition is similar when HT patients are compared to GD and NTMG to PTC patients and differs when AITD are compared with non-AITD patients. In PTC-T patients, it is similar to that of AITD patients. SN - 0021-972X UR - https://www.unboundmedicine.com/medline/citation/18029466/Characterization_of_thyroglobulin_epitopes_in_patients_with_autoimmune_and_non_autoimmune_thyroid_diseases_using_recombinant_human_monoclonal_thyroglobulin_autoantibodies_ L2 - https://academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2007-1199 DB - PRIME DP - Unbound Medicine ER -