Tags

Type your tag names separated by a space and hit enter

Determining IgA and IgG antigliadin, IgA antitransglutaminase, and antiendomysial antibodies in monkey esophagus and in umbilical cord for diagnosis of celiac disease in developing countries.
J Pediatr Gastroenterol Nutr. 2007 Nov; 45(5):551-8.JP

Abstract

OBJECTIVES

To assess the efficiency of determining IgA and IgG antigliadin antibodies (IgA- and IgG-AGA, respectively), antitransglutaminase (TgA), and anti-endomysial antibodies (AEA) in human umbilical cord (CO) and monkey esophagus for diagnosis of celiac disease; to determine the correlation between serological markers and celiac disease.

PATIENTS AND METHODS

A total of 400 patients were divided in 3 groups: group 1 with 37 patients with celiac disease, group 2 with 208 patients with no enteropathies, and group 3 with 155 patients with other enteropathies. IgA-AGA, IgG-AGA, and TgA were assessed using enzyme-linked immunosorbent assay, whereas AEA was evaluated by indirect immunofluorescence.

RESULTS

Sensitivity and specificity of IgA-AGA were 81.1% and 95.2%, of IgG-AGA 89.2% and 95.2%, of TgA 83.9% and 96.8%, of AEA-CO 87.9% and 100%, and of AEA of monkey esophagus 88.6% and 100%, respectively. Positive predictive values were 75.0%, 76.7%, 83.9%, and 100%. Negative predictive values were 96.6%, 98.0%, 96.8%, and 97.7% for IgA-AGA, IgG-AGA, TgA, and AEA, respectively. Multivariate analysis showed a strong association between AEA-CO and celiac disease and a good correlation with other markers (TgA, IgA-AGA, and IgG-AGA).

CONCLUSIONS

TgA has been recommended for screening patients with celiac disease. Considering the similar sensitivity and specificity of IgA-AGA and TgA and their correlations in the multivariate analysis, both are applicable for this purpose. However, because TgA tests are highly costly and celiac disease is associated with IgA deficiency, the determination of IgA-AGA and IgG-AGA, followed by AEA-CO, is suitable for screening in developing countries, provided a cutoff point for these examinations is established. The results of antiendomysial antibodies in umbilical cord overlapped those in monkey esophagus. Therefore, umbilical cord should be used as a substrate instead of specimens from endangered species.

Authors+Show Affiliations

Departments of Complementary Medicine, FM/UFMG, Avenida Alfredo Balena 190, s/6000, 30130-100, Belo Horizonte, Minas Gerais, Brazil. magbahia@medicina.ufmg.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18030232

Citation

Bahia, Magda, et al. "Determining IgA and IgG Antigliadin, IgA Antitransglutaminase, and Antiendomysial Antibodies in Monkey Esophagus and in Umbilical Cord for Diagnosis of Celiac Disease in Developing Countries." Journal of Pediatric Gastroenterology and Nutrition, vol. 45, no. 5, 2007, pp. 551-8.
Bahia M, Penna FJ, Sampaio IB, et al. Determining IgA and IgG antigliadin, IgA antitransglutaminase, and antiendomysial antibodies in monkey esophagus and in umbilical cord for diagnosis of celiac disease in developing countries. J Pediatr Gastroenterol Nutr. 2007;45(5):551-8.
Bahia, M., Penna, F. J., Sampaio, I. B., Silva, G. M., & Andrade, E. M. (2007). Determining IgA and IgG antigliadin, IgA antitransglutaminase, and antiendomysial antibodies in monkey esophagus and in umbilical cord for diagnosis of celiac disease in developing countries. Journal of Pediatric Gastroenterology and Nutrition, 45(5), 551-8.
Bahia M, et al. Determining IgA and IgG Antigliadin, IgA Antitransglutaminase, and Antiendomysial Antibodies in Monkey Esophagus and in Umbilical Cord for Diagnosis of Celiac Disease in Developing Countries. J Pediatr Gastroenterol Nutr. 2007;45(5):551-8. PubMed PMID: 18030232.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determining IgA and IgG antigliadin, IgA antitransglutaminase, and antiendomysial antibodies in monkey esophagus and in umbilical cord for diagnosis of celiac disease in developing countries. AU - Bahia,Magda, AU - Penna,Francisco José, AU - Sampaio,Ivan Barbosa Machado, AU - Silva,Geraldo Magela Fritz, AU - Andrade,Eugênio Marcos Goulart, PY - 2007/11/22/pubmed PY - 2008/1/16/medline PY - 2007/11/22/entrez SP - 551 EP - 8 JF - Journal of pediatric gastroenterology and nutrition JO - J. Pediatr. Gastroenterol. Nutr. VL - 45 IS - 5 N2 - OBJECTIVES: To assess the efficiency of determining IgA and IgG antigliadin antibodies (IgA- and IgG-AGA, respectively), antitransglutaminase (TgA), and anti-endomysial antibodies (AEA) in human umbilical cord (CO) and monkey esophagus for diagnosis of celiac disease; to determine the correlation between serological markers and celiac disease. PATIENTS AND METHODS: A total of 400 patients were divided in 3 groups: group 1 with 37 patients with celiac disease, group 2 with 208 patients with no enteropathies, and group 3 with 155 patients with other enteropathies. IgA-AGA, IgG-AGA, and TgA were assessed using enzyme-linked immunosorbent assay, whereas AEA was evaluated by indirect immunofluorescence. RESULTS: Sensitivity and specificity of IgA-AGA were 81.1% and 95.2%, of IgG-AGA 89.2% and 95.2%, of TgA 83.9% and 96.8%, of AEA-CO 87.9% and 100%, and of AEA of monkey esophagus 88.6% and 100%, respectively. Positive predictive values were 75.0%, 76.7%, 83.9%, and 100%. Negative predictive values were 96.6%, 98.0%, 96.8%, and 97.7% for IgA-AGA, IgG-AGA, TgA, and AEA, respectively. Multivariate analysis showed a strong association between AEA-CO and celiac disease and a good correlation with other markers (TgA, IgA-AGA, and IgG-AGA). CONCLUSIONS: TgA has been recommended for screening patients with celiac disease. Considering the similar sensitivity and specificity of IgA-AGA and TgA and their correlations in the multivariate analysis, both are applicable for this purpose. However, because TgA tests are highly costly and celiac disease is associated with IgA deficiency, the determination of IgA-AGA and IgG-AGA, followed by AEA-CO, is suitable for screening in developing countries, provided a cutoff point for these examinations is established. The results of antiendomysial antibodies in umbilical cord overlapped those in monkey esophagus. Therefore, umbilical cord should be used as a substrate instead of specimens from endangered species. SN - 1536-4801 UR - https://www.unboundmedicine.com/medline/citation/18030232/Determining_IgA_and_IgG_antigliadin_IgA_antitransglutaminase_and_antiendomysial_antibodies_in_monkey_esophagus_and_in_umbilical_cord_for_diagnosis_of_celiac_disease_in_developing_countries_ DB - PRIME DP - Unbound Medicine ER -