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Assessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria.
Trans R Soc Trop Med Hyg. 2008 Jan; 102(1):25-31.TR

Abstract

A study to assess the diagnostic capabilities of three parasite lactate dehydrogenase (pan-pLDH) tests, Vistapan), Carestart and Parabank), was conducted in Uganda. An HRP2 test, Paracheck-Pf), and a Giemsa-stained blood film were performed with the pLDH tests for outpatients with suspected malaria. In total, 460 subjects were recruited: 248 with positive blood films and 212 with negative blood films. Plasmodium falciparum was present in 95% of infections. Sensitivity above 90% was shown by two pLDH tests, Carestart (95.6%) and Vistapan (91.9%), and specificity above 90% by Parabank (94.3%) and Carestart (91.5%). Sensitivity decreased with low parasitaemia (chi(2) trend, P<0.001); however, all tests achieved sensitivity >90% with parasitaemia > or =100/microl. All tests had good inter-reader reliability (kappa>0.95). Two weeks after diagnosis, 4-10% of pLDH tests were still positive compared with 69.7% of the HRP2 tests. All tests had similar ease of use. In conclusion, two pLDH tests performed well in diagnosing P. falciparum malaria, and all pLDH tests became negative after treatment more quickly than the HRP2. Therefore the rapid test of choice for use with artemisinin-combination therapies in this area would be one of these new pLDH tests.

Authors+Show Affiliations

Fogg C
Epicentre, 8 rue Saint Sabin, 75011, Paris, France. carolefogg@yahoo.co.uk
Twesigye R
No affiliation info available
Batwala V
No affiliation info available
Piola P
No affiliation info available
Nabasumba C
No affiliation info available
Kiguli J
No affiliation info available
Mutebi F
No affiliation info available
Hook C
No affiliation info available
Guillerm M
No affiliation info available
Moody A
No affiliation info available
Guthmann JP
No affiliation info available

MeSH

AdolescentAdultAnimalsChildChild, PreschoolClinical Enzyme TestsFemaleHumansInfantL-Lactate DehydrogenaseMalariaMaleParasitemiaPlasmodium falciparumReagent Kits, DiagnosticSensitivity and SpecificityUganda

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18031779

Citation

Fogg, Carole, et al. "Assessment of Three New Parasite Lactate Dehydrogenase (pan-pLDH) Tests for Diagnosis of Uncomplicated Malaria." Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 102, no. 1, 2008, pp. 25-31.
Fogg C, Twesigye R, Batwala V, et al. Assessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria. Trans R Soc Trop Med Hyg. 2008;102(1):25-31.
Fogg, C., Twesigye, R., Batwala, V., Piola, P., Nabasumba, C., Kiguli, J., Mutebi, F., Hook, C., Guillerm, M., Moody, A., & Guthmann, J. P. (2008). Assessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria. Transactions of the Royal Society of Tropical Medicine and Hygiene, 102(1), 25-31.
Fogg C, et al. Assessment of Three New Parasite Lactate Dehydrogenase (pan-pLDH) Tests for Diagnosis of Uncomplicated Malaria. Trans R Soc Trop Med Hyg. 2008;102(1):25-31. PubMed PMID: 18031779.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria. AU - Fogg,Carole, AU - Twesigye,Rogers, AU - Batwala,Vincent, AU - Piola,Patrice, AU - Nabasumba,Carolyn, AU - Kiguli,James, AU - Mutebi,Frederick, AU - Hook,Christa, AU - Guillerm,Martine, AU - Moody,Anthony, AU - Guthmann,Jean-Paul, Y1 - 2007/11/26/ PY - 2006/10/23/received PY - 2007/09/24/revised PY - 2007/09/25/accepted PY - 2007/11/23/pubmed PY - 2008/3/5/medline PY - 2007/11/23/entrez SP - 25 EP - 31 JF - Transactions of the Royal Society of Tropical Medicine and Hygiene JO - Trans R Soc Trop Med Hyg VL - 102 IS - 1 N2 - A study to assess the diagnostic capabilities of three parasite lactate dehydrogenase (pan-pLDH) tests, Vistapan), Carestart and Parabank), was conducted in Uganda. An HRP2 test, Paracheck-Pf), and a Giemsa-stained blood film were performed with the pLDH tests for outpatients with suspected malaria. In total, 460 subjects were recruited: 248 with positive blood films and 212 with negative blood films. Plasmodium falciparum was present in 95% of infections. Sensitivity above 90% was shown by two pLDH tests, Carestart (95.6%) and Vistapan (91.9%), and specificity above 90% by Parabank (94.3%) and Carestart (91.5%). Sensitivity decreased with low parasitaemia (chi(2) trend, P<0.001); however, all tests achieved sensitivity >90% with parasitaemia > or =100/microl. All tests had good inter-reader reliability (kappa>0.95). Two weeks after diagnosis, 4-10% of pLDH tests were still positive compared with 69.7% of the HRP2 tests. All tests had similar ease of use. In conclusion, two pLDH tests performed well in diagnosing P. falciparum malaria, and all pLDH tests became negative after treatment more quickly than the HRP2. Therefore the rapid test of choice for use with artemisinin-combination therapies in this area would be one of these new pLDH tests. SN - 0035-9203 UR - https://www.unboundmedicine.com/medline/citation/18031779/Assessment_of_three_new_parasite_lactate_dehydrogenase__pan_pLDH__tests_for_diagnosis_of_uncomplicated_malaria_ DB - PRIME DP - Unbound Medicine ER -