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Genomic response programs of Saccharomyces cerevisiae following protoplasting and regeneration.
Fungal Genet Biol. 2008 Mar; 45(3):253-65.FG

Abstract

Global transcription profiling during regeneration of Saccharomyces cerevisiae protoplasts was explored. DNA microarrays measured the expression of 6388 genes and wall removal resulted initially in over-expression of 861 genes that decayed later on, a behaviour expected from a transient stress response. Kinetics of expression divided the genes into 25 clusters. Transcription of the genes from clusters 14-25 was initially up-regulated, suggesting that the grouped genes permitted cell adaptation to the removal of the wall. Clustering of genes involved in "wall structure and biosynthesis" showed that most of them had initially low levels of expression that increased along the process. Analysis by use of the T-profiler showed that the group of "structural components of the wall" was up-regulated after two hours and remained as such during the process. These results evoke the likeness and difference with the process occurring in Candida albicans.

Authors+Show Affiliations

Castillo L
Departament de Microbiología i Ecología, Facultat de Farmacia, Universitat de València, Avgda. Vicent Andrés Estellés s/n, 46100 Burjassot, València, Spain.
Martínez AI
No affiliation info available
Gelis S
No affiliation info available
Ruiz-Herrera J
No affiliation info available
Valentín E
No affiliation info available
Sentandreu R
No affiliation info available

MeSH

Cell WallGene Expression ProfilingGene Expression Regulation, FungalGenomicsOligonucleotide Array Sequence AnalysisProtoplastsReverse Transcriptase Polymerase Chain ReactionSaccharomyces cerevisiaeSaccharomyces cerevisiae Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18032075

Citation

Castillo, Luis, et al. "Genomic Response Programs of Saccharomyces Cerevisiae Following Protoplasting and Regeneration." Fungal Genetics and Biology : FG & B, vol. 45, no. 3, 2008, pp. 253-65.
Castillo L, Martínez AI, Gelis S, et al. Genomic response programs of Saccharomyces cerevisiae following protoplasting and regeneration. Fungal Genet Biol. 2008;45(3):253-65.
Castillo, L., Martínez, A. I., Gelis, S., Ruiz-Herrera, J., Valentín, E., & Sentandreu, R. (2008). Genomic response programs of Saccharomyces cerevisiae following protoplasting and regeneration. Fungal Genetics and Biology : FG & B, 45(3), 253-65.
Castillo L, et al. Genomic Response Programs of Saccharomyces Cerevisiae Following Protoplasting and Regeneration. Fungal Genet Biol. 2008;45(3):253-65. PubMed PMID: 18032075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic response programs of Saccharomyces cerevisiae following protoplasting and regeneration. AU - Castillo,Luis, AU - Martínez,Ana Isabel, AU - Gelis,Samuel, AU - Ruiz-Herrera,José, AU - Valentín,Eulogio, AU - Sentandreu,Rafael, Y1 - 2007/10/12/ PY - 2007/04/23/received PY - 2007/10/04/revised PY - 2007/10/05/accepted PY - 2007/11/23/pubmed PY - 2008/3/7/medline PY - 2007/11/23/entrez SP - 253 EP - 65 JF - Fungal genetics and biology : FG & B JO - Fungal Genet Biol VL - 45 IS - 3 N2 - Global transcription profiling during regeneration of Saccharomyces cerevisiae protoplasts was explored. DNA microarrays measured the expression of 6388 genes and wall removal resulted initially in over-expression of 861 genes that decayed later on, a behaviour expected from a transient stress response. Kinetics of expression divided the genes into 25 clusters. Transcription of the genes from clusters 14-25 was initially up-regulated, suggesting that the grouped genes permitted cell adaptation to the removal of the wall. Clustering of genes involved in "wall structure and biosynthesis" showed that most of them had initially low levels of expression that increased along the process. Analysis by use of the T-profiler showed that the group of "structural components of the wall" was up-regulated after two hours and remained as such during the process. These results evoke the likeness and difference with the process occurring in Candida albicans. SN - 1096-0937 UR - https://www.unboundmedicine.com/medline/citation/18032075/Genomic_response_programs_of_Saccharomyces_cerevisiae_following_protoplasting_and_regeneration_ DB - PRIME DP - Unbound Medicine ER -