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Transient receptor potential vanilloid type 1 channels act as mechanoreceptors and cause substance P release and sensory activation in rat kidneys.
Am J Physiol Renal Physiol. 2008 Feb; 294(2):F316-25.AJ

Abstract

Stimulation of capsaicin receptors results in an increase in afferent renal nerve activity (ARNA), but it is unclear how capsaicin contributes to sensory activation intrarenally. Here, we studied the relationships between capsaicin receptor activation, substance P (SP) release, and the sensory response in the rat renal pelvis. Immunoblots showed that one of the capsaicin receptors, transient receptor potential vanilloid type 1 channel (TRPV1), was found in various renal tissues and was especially abundant in the renal pelvis, where most sensory nerve fibers originate. Interestingly, immunolabeling showed colocalization of TRPV1, SP, and the panneuronal marker PGP9.5 in the renal pelvis. Electrophysiological recordings showed that SP and capsaicin activated the same mechanosensitive ARNA in a single-unit preparation. Intrapelvic administration of capsaicin or a specific TRPV1 agonist, resiniferatoxin, resulted in a dose-dependent increase in multi-unit ARNA and SP release, and these effects were blocked by the TRVP1 blocker capsazepine. Inhibition of the SP receptor by L-703,606 largely prevented capsaicin- or resiniferatoxin-induced ARNA. Capsazepine also prevented intrapelvic pressure (IPP)-dependent ARNA activation and contralateral diuresis/natriuresis in the renorenal reflex at an IPP of 20 mmHg, but had no effect at an IPP of 50 mmHg. These data indicate that TRPV1, a low-pressure baroreceptor, is present in the renal pelvis and exclusively regulates neuropeptide release from primary renal afferent C-fibers in response to mechanostimulation.

Authors+Show Affiliations

Division of Chest Medicine, Department of Internal Medicine, Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18032552

Citation

Feng, Nan-Hsiung, et al. "Transient Receptor Potential Vanilloid Type 1 Channels Act as Mechanoreceptors and Cause Substance P Release and Sensory Activation in Rat Kidneys." American Journal of Physiology. Renal Physiology, vol. 294, no. 2, 2008, pp. F316-25.
Feng NH, Lee HH, Shiang JC, et al. Transient receptor potential vanilloid type 1 channels act as mechanoreceptors and cause substance P release and sensory activation in rat kidneys. Am J Physiol Renal Physiol. 2008;294(2):F316-25.
Feng, N. H., Lee, H. H., Shiang, J. C., & Ma, M. C. (2008). Transient receptor potential vanilloid type 1 channels act as mechanoreceptors and cause substance P release and sensory activation in rat kidneys. American Journal of Physiology. Renal Physiology, 294(2), F316-25.
Feng NH, et al. Transient Receptor Potential Vanilloid Type 1 Channels Act as Mechanoreceptors and Cause Substance P Release and Sensory Activation in Rat Kidneys. Am J Physiol Renal Physiol. 2008;294(2):F316-25. PubMed PMID: 18032552.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transient receptor potential vanilloid type 1 channels act as mechanoreceptors and cause substance P release and sensory activation in rat kidneys. AU - Feng,Nan-Hsiung, AU - Lee,Hsang-Hsing, AU - Shiang,Jeng-Chaun, AU - Ma,Ming-Chieh, Y1 - 2007/11/21/ PY - 2007/11/23/pubmed PY - 2008/4/25/medline PY - 2007/11/23/entrez SP - F316 EP - 25 JF - American journal of physiology. Renal physiology JO - Am J Physiol Renal Physiol VL - 294 IS - 2 N2 - Stimulation of capsaicin receptors results in an increase in afferent renal nerve activity (ARNA), but it is unclear how capsaicin contributes to sensory activation intrarenally. Here, we studied the relationships between capsaicin receptor activation, substance P (SP) release, and the sensory response in the rat renal pelvis. Immunoblots showed that one of the capsaicin receptors, transient receptor potential vanilloid type 1 channel (TRPV1), was found in various renal tissues and was especially abundant in the renal pelvis, where most sensory nerve fibers originate. Interestingly, immunolabeling showed colocalization of TRPV1, SP, and the panneuronal marker PGP9.5 in the renal pelvis. Electrophysiological recordings showed that SP and capsaicin activated the same mechanosensitive ARNA in a single-unit preparation. Intrapelvic administration of capsaicin or a specific TRPV1 agonist, resiniferatoxin, resulted in a dose-dependent increase in multi-unit ARNA and SP release, and these effects were blocked by the TRVP1 blocker capsazepine. Inhibition of the SP receptor by L-703,606 largely prevented capsaicin- or resiniferatoxin-induced ARNA. Capsazepine also prevented intrapelvic pressure (IPP)-dependent ARNA activation and contralateral diuresis/natriuresis in the renorenal reflex at an IPP of 20 mmHg, but had no effect at an IPP of 50 mmHg. These data indicate that TRPV1, a low-pressure baroreceptor, is present in the renal pelvis and exclusively regulates neuropeptide release from primary renal afferent C-fibers in response to mechanostimulation. SN - 1931-857X UR - https://www.unboundmedicine.com/medline/citation/18032552/Transient_receptor_potential_vanilloid_type_1_channels_act_as_mechanoreceptors_and_cause_substance_P_release_and_sensory_activation_in_rat_kidneys_ L2 - https://journals.physiology.org/doi/10.1152/ajprenal.00308.2007?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -