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Immunoassay with cytomegalovirus early antigens from gene products p52 and CM2 (UL44 and UL57) detects active infection in patients with chronic fatigue syndrome.
J Clin Pathol. 2008 May; 61(5):623-6.JC

Abstract

AIMS

To investigate whether the use of recombinant early antigens for detection of antibodies to human cytomegalovirus (HCMV) gene products CM(2) (UL44, UL57) and p52 (UL44) is specific in the diagnosis and differentiation of active HCMV infection in a subset of patients with chronic fatigue syndrome (CFS), a diagnosis which is often missed by the current ELISA assay that uses crude viral lysate antigen.

METHODS

At a single clinic from 1999 to 2001, a total of 4774 serological tests were performed in 1135 patients with patients using two immunoassays, Copalis and ELISA. The Copalis immunoassay utilised HCMV early gene products of UL44 and UL57 recombinant antigens for detection of HCMV IgM antibody, and viral capsid antigen for detection of HCMV IgG antibody. The ELISA immunoassay utilised viral crude lysate as antigen for detection of both HCMV IgG and IgM.

RESULTS

517 patients (45.6%) were positive for HCMV IgG by both assays. Of these, 12 (2.2%) were positive for HCMV(V) IgM serum antibody by HCMV ELISA assay, and 61 (11.8%) were positive for IgM HCMV serum antibody by Copalis assay. The Copalis assay that uses HCMV early recombinant gene products CM(2) (UL44, UL57) and p52 (UL44) in comparison with ELISA was 98% specific.

CONCLUSIONS

Immunoassays that use early antigen recombinant HCMV CM(2) and p52 are five times more sensitive than HCMV ELISA assay using viral lysate, and are specific in the detection and differentiation of active HCMV infection in a subset of patients with CFS.

Authors+Show Affiliations

Pathgroup Labs, Nashville, TN, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18037660

Citation

Beqaj, S H., et al. "Immunoassay With Cytomegalovirus Early Antigens From Gene Products P52 and CM2 (UL44 and UL57) Detects Active Infection in Patients With Chronic Fatigue Syndrome." Journal of Clinical Pathology, vol. 61, no. 5, 2008, pp. 623-6.
Beqaj SH, Lerner AM, Fitzgerald JT. Immunoassay with cytomegalovirus early antigens from gene products p52 and CM2 (UL44 and UL57) detects active infection in patients with chronic fatigue syndrome. J Clin Pathol. 2008;61(5):623-6.
Beqaj, S. H., Lerner, A. M., & Fitzgerald, J. T. (2008). Immunoassay with cytomegalovirus early antigens from gene products p52 and CM2 (UL44 and UL57) detects active infection in patients with chronic fatigue syndrome. Journal of Clinical Pathology, 61(5), 623-6.
Beqaj SH, Lerner AM, Fitzgerald JT. Immunoassay With Cytomegalovirus Early Antigens From Gene Products P52 and CM2 (UL44 and UL57) Detects Active Infection in Patients With Chronic Fatigue Syndrome. J Clin Pathol. 2008;61(5):623-6. PubMed PMID: 18037660.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunoassay with cytomegalovirus early antigens from gene products p52 and CM2 (UL44 and UL57) detects active infection in patients with chronic fatigue syndrome. AU - Beqaj,S H, AU - Lerner,A M, AU - Fitzgerald,J T, Y1 - 2007/11/23/ PY - 2007/11/27/pubmed PY - 2008/5/30/medline PY - 2007/11/27/entrez SP - 623 EP - 6 JF - Journal of clinical pathology JO - J Clin Pathol VL - 61 IS - 5 N2 - AIMS: To investigate whether the use of recombinant early antigens for detection of antibodies to human cytomegalovirus (HCMV) gene products CM(2) (UL44, UL57) and p52 (UL44) is specific in the diagnosis and differentiation of active HCMV infection in a subset of patients with chronic fatigue syndrome (CFS), a diagnosis which is often missed by the current ELISA assay that uses crude viral lysate antigen. METHODS: At a single clinic from 1999 to 2001, a total of 4774 serological tests were performed in 1135 patients with patients using two immunoassays, Copalis and ELISA. The Copalis immunoassay utilised HCMV early gene products of UL44 and UL57 recombinant antigens for detection of HCMV IgM antibody, and viral capsid antigen for detection of HCMV IgG antibody. The ELISA immunoassay utilised viral crude lysate as antigen for detection of both HCMV IgG and IgM. RESULTS: 517 patients (45.6%) were positive for HCMV IgG by both assays. Of these, 12 (2.2%) were positive for HCMV(V) IgM serum antibody by HCMV ELISA assay, and 61 (11.8%) were positive for IgM HCMV serum antibody by Copalis assay. The Copalis assay that uses HCMV early recombinant gene products CM(2) (UL44, UL57) and p52 (UL44) in comparison with ELISA was 98% specific. CONCLUSIONS: Immunoassays that use early antigen recombinant HCMV CM(2) and p52 are five times more sensitive than HCMV ELISA assay using viral lysate, and are specific in the detection and differentiation of active HCMV infection in a subset of patients with CFS. SN - 1472-4146 UR - https://www.unboundmedicine.com/medline/citation/18037660/Immunoassay_with_cytomegalovirus_early_antigens_from_gene_products_p52_and_CM2__UL44_and_UL57__detects_active_infection_in_patients_with_chronic_fatigue_syndrome_ L2 - https://jcp.bmj.com/lookup/pmidlookup?view=long&pmid=18037660 DB - PRIME DP - Unbound Medicine ER -