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Oxidative DNA damage preventive activity and antioxidant potential of Stevia rebaudiana (Bertoni) Bertoni, a natural sweetener.
J Agric Food Chem. 2007 Dec 26; 55(26):10962-7.JA

Abstract

At 0.1 mg/mL, the ethyl acetate extract (EAE) of the crude 85% methanolic extract (CAE) of Stevia rebaudiana leaves exhibited preventive activity against DNA strand scission by *OH generated in Fenton's reaction on pBluescript II SK (-) DNA. Its efficacy is better than that of quercetin. The radical scavenging capacity of CAE was evaluated by the DPPH test (IC50=47.66+/-1.04 microg/mL). EAE was derived from CAE scavenged DPPH (IC50=9.26+/-0.04 microg/mL), ABTS+ (IC50=3.04+/-0.22 microg/mL) and *OH (IC50=3.08+/-0.19 microg/mL). Additionally, inhibition of lipid peroxidation induced with 25 mM FeSO 4 on rat liver homogenate as a lipid source was noted with CAE (IC50=2.1+/-1.07 mg/mL). The total polyphenols and total flavonoids of EAE were 0.86 mg gallic acid equivalents/mg and 0.83 mg of quercetin equivalents/mg, respectively. Flavonoids, isolated from EAE, were characterized as quercetin-3-O-arabinoside, quercitrin, apigenin, apigenin-4-O-glucoside, luteolin, and kaempferol-3-O-rhamnoside by LC-MS and NMR analysis. These results indicate that Stevia rebaudiana may be useful as a potential source of natural antioxidants.

Authors+Show Affiliations

Drug Development/Diagnositcs and Biotechnology Division, Indian Institute of Chemical Biology, Kolkata, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18038982

Citation

Ghanta, Srijani, et al. "Oxidative DNA Damage Preventive Activity and Antioxidant Potential of Stevia Rebaudiana (Bertoni) Bertoni, a Natural Sweetener." Journal of Agricultural and Food Chemistry, vol. 55, no. 26, 2007, pp. 10962-7.
Ghanta S, Banerjee A, Poddar A, et al. Oxidative DNA damage preventive activity and antioxidant potential of Stevia rebaudiana (Bertoni) Bertoni, a natural sweetener. J Agric Food Chem. 2007;55(26):10962-7.
Ghanta, S., Banerjee, A., Poddar, A., & Chattopadhyay, S. (2007). Oxidative DNA damage preventive activity and antioxidant potential of Stevia rebaudiana (Bertoni) Bertoni, a natural sweetener. Journal of Agricultural and Food Chemistry, 55(26), 10962-7.
Ghanta S, et al. Oxidative DNA Damage Preventive Activity and Antioxidant Potential of Stevia Rebaudiana (Bertoni) Bertoni, a Natural Sweetener. J Agric Food Chem. 2007 Dec 26;55(26):10962-7. PubMed PMID: 18038982.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative DNA damage preventive activity and antioxidant potential of Stevia rebaudiana (Bertoni) Bertoni, a natural sweetener. AU - Ghanta,Srijani, AU - Banerjee,Anindita, AU - Poddar,Avijit, AU - Chattopadhyay,Sharmila, Y1 - 2007/11/27/ PY - 2007/11/28/pubmed PY - 2008/2/29/medline PY - 2007/11/28/entrez SP - 10962 EP - 7 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 55 IS - 26 N2 - At 0.1 mg/mL, the ethyl acetate extract (EAE) of the crude 85% methanolic extract (CAE) of Stevia rebaudiana leaves exhibited preventive activity against DNA strand scission by *OH generated in Fenton's reaction on pBluescript II SK (-) DNA. Its efficacy is better than that of quercetin. The radical scavenging capacity of CAE was evaluated by the DPPH test (IC50=47.66+/-1.04 microg/mL). EAE was derived from CAE scavenged DPPH (IC50=9.26+/-0.04 microg/mL), ABTS+ (IC50=3.04+/-0.22 microg/mL) and *OH (IC50=3.08+/-0.19 microg/mL). Additionally, inhibition of lipid peroxidation induced with 25 mM FeSO 4 on rat liver homogenate as a lipid source was noted with CAE (IC50=2.1+/-1.07 mg/mL). The total polyphenols and total flavonoids of EAE were 0.86 mg gallic acid equivalents/mg and 0.83 mg of quercetin equivalents/mg, respectively. Flavonoids, isolated from EAE, were characterized as quercetin-3-O-arabinoside, quercitrin, apigenin, apigenin-4-O-glucoside, luteolin, and kaempferol-3-O-rhamnoside by LC-MS and NMR analysis. These results indicate that Stevia rebaudiana may be useful as a potential source of natural antioxidants. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/18038982/Oxidative_DNA_damage_preventive_activity_and_antioxidant_potential_of_Stevia_rebaudiana__Bertoni__Bertoni_a_natural_sweetener_ L2 - https://doi.org/10.1021/jf071892q DB - PRIME DP - Unbound Medicine ER -