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[Effects of acupoint-embedding of medicinal thread and electroacupuncture on contents of TNF-alpha and IL-6 of hippocampus tissue in rats with focal cerebral ischemia-reperfusion injury].
Zhen Ci Yan Jiu. 2007 Oct; 32(5):301-5.ZC

Abstract

OBJECTIVE

To observe the effects of acupoint-embedding (AE) of collagen plus Tetramethylpyrazine and electroacupuncture (EA) on the contents of TNF-alpha and IL-6 in hippocampus tissue in rats with focal cerebral ischemia-reperfusion (CI-R) injury.

METHODS

A total of 120 SD rats were randomly divided into normal control (n=8), sham operation (n=24), model (CI-R, n=24), EA (n=24) and AE (n=24) groups, and the later 4 groups were further divided into 24 h, 72 h and 120 h subgroups (time-points) separately, with 8 cases in each. CI-R model was established by middle cerebral artery occlusion (MCAO) and reperfusion. For animals of AE group, the medicinal thread consisting of collagen and Tetramethylpyrazine was implanted into "Dazhui" (GV14) and bilateral "Neiguan" (PC6), once daily. For rats of EA group, EA (120 cycles/min, 1 mA) was applied to GV14 and PC6 for 30 min, once daily. The contents of TNF-alpha and IL-6 in the hippocampus were detected by using radio-immunoassay after processing the tissue samples (homogenate, centrifuge).

RESULTS

The contents of TNF-alpha and IL-6 in hippocampus tissue of model group at 24 h, 72 h and 120 h time-points were significantly higher than those of normal control and sham operation groups (P<0.05), while after EA and AE, the contents of TNF-alpha at the 3 time-points decreased significantly (P<0.05), and IL-6 contents at the same 3 time-points increased further and significantly (P<0.05). Comparison between EA and AE groups showed that the contents of TNF-alpha of the later group at 72 h and 120 h were significantly lower than those of EA group (P<0.05), while IL-6 contents of AE group were significantly higher than those of EA group at the same time-points (P<0.05).

CONCLUSION

Both EA and AE of collagen and Tetramethylpyrazine can evidently lower TNF-alpha content and significantly raise IL-6 level in hippocampus in rats with cerebral ischemia/reperfusion injury and the effect of AE is significantly superior to that of EA, which may contribute to their protective effects on ischemic cerebral neurons.

Authors+Show Affiliations

Hubei College of Chinese Medicine, Wuhan 430061, China. xiyu1618@sina.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18050620

Citation

Kong, Li-Hong, et al. "[Effects of Acupoint-embedding of Medicinal Thread and Electroacupuncture On Contents of TNF-alpha and IL-6 of Hippocampus Tissue in Rats With Focal Cerebral Ischemia-reperfusion Injury]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 32, no. 5, 2007, pp. 301-5.
Kong LH, Sun GJ, Mao JJ, et al. [Effects of acupoint-embedding of medicinal thread and electroacupuncture on contents of TNF-alpha and IL-6 of hippocampus tissue in rats with focal cerebral ischemia-reperfusion injury]. Zhen Ci Yan Jiu. 2007;32(5):301-5.
Kong, L. H., Sun, G. J., Mao, J. J., Su, F. R., & Gao, S. (2007). [Effects of acupoint-embedding of medicinal thread and electroacupuncture on contents of TNF-alpha and IL-6 of hippocampus tissue in rats with focal cerebral ischemia-reperfusion injury]. Zhen Ci Yan Jiu = Acupuncture Research, 32(5), 301-5.
Kong LH, et al. [Effects of Acupoint-embedding of Medicinal Thread and Electroacupuncture On Contents of TNF-alpha and IL-6 of Hippocampus Tissue in Rats With Focal Cerebral Ischemia-reperfusion Injury]. Zhen Ci Yan Jiu. 2007;32(5):301-5. PubMed PMID: 18050620.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of acupoint-embedding of medicinal thread and electroacupuncture on contents of TNF-alpha and IL-6 of hippocampus tissue in rats with focal cerebral ischemia-reperfusion injury]. AU - Kong,Li-Hong, AU - Sun,Guo-Jie, AU - Mao,Juan-Juan, AU - Su,Fu-Rong, AU - Gao,Shan, PY - 2007/12/7/pubmed PY - 2007/12/21/medline PY - 2007/12/7/entrez SP - 301 EP - 5 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 32 IS - 5 N2 - OBJECTIVE: To observe the effects of acupoint-embedding (AE) of collagen plus Tetramethylpyrazine and electroacupuncture (EA) on the contents of TNF-alpha and IL-6 in hippocampus tissue in rats with focal cerebral ischemia-reperfusion (CI-R) injury. METHODS: A total of 120 SD rats were randomly divided into normal control (n=8), sham operation (n=24), model (CI-R, n=24), EA (n=24) and AE (n=24) groups, and the later 4 groups were further divided into 24 h, 72 h and 120 h subgroups (time-points) separately, with 8 cases in each. CI-R model was established by middle cerebral artery occlusion (MCAO) and reperfusion. For animals of AE group, the medicinal thread consisting of collagen and Tetramethylpyrazine was implanted into "Dazhui" (GV14) and bilateral "Neiguan" (PC6), once daily. For rats of EA group, EA (120 cycles/min, 1 mA) was applied to GV14 and PC6 for 30 min, once daily. The contents of TNF-alpha and IL-6 in the hippocampus were detected by using radio-immunoassay after processing the tissue samples (homogenate, centrifuge). RESULTS: The contents of TNF-alpha and IL-6 in hippocampus tissue of model group at 24 h, 72 h and 120 h time-points were significantly higher than those of normal control and sham operation groups (P<0.05), while after EA and AE, the contents of TNF-alpha at the 3 time-points decreased significantly (P<0.05), and IL-6 contents at the same 3 time-points increased further and significantly (P<0.05). Comparison between EA and AE groups showed that the contents of TNF-alpha of the later group at 72 h and 120 h were significantly lower than those of EA group (P<0.05), while IL-6 contents of AE group were significantly higher than those of EA group at the same time-points (P<0.05). CONCLUSION: Both EA and AE of collagen and Tetramethylpyrazine can evidently lower TNF-alpha content and significantly raise IL-6 level in hippocampus in rats with cerebral ischemia/reperfusion injury and the effect of AE is significantly superior to that of EA, which may contribute to their protective effects on ischemic cerebral neurons. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/18050620/[Effects_of_acupoint_embedding_of_medicinal_thread_and_electroacupuncture_on_contents_of_TNF_alpha_and_IL_6_of_hippocampus_tissue_in_rats_with_focal_cerebral_ischemia_reperfusion_injury]_ DB - PRIME DP - Unbound Medicine ER -