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[Interferon gamma (IFN-gamma) level in broncholaveolar lavage (BAL) fluid is positively correlated with CD4/CD8 ratio in selected interstitial lung diseases].
Pol Merkur Lekarski. 2007 Jul; 23(133):15-21.PM

Abstract

IFN-gamma a potent antifibrotic activity in interstitial lung diseases (ILD). T cells, both Th1 and Tc1, are considered to be the main local source of IFN-gamma.

MATERIAL AND METHODS

BAL fluids of 98 patients with ILD, incl. idiopathic pulmonary fibrosis (IPF/UIP), sarcoidosis, extrinsic allergic alveolitis (EAA), asbestosis and silicosis (n=16, 49, 7, 10, 16 resp.) were tested with ELISA for IFN-gamma levels. Results were compared with BAL cytoimmunology and patients' clinical data.

RESULTS

Significantly increased IFN-gamma levels were found in non-treated patients with EAA (7.8 +/- 2.1), IPF (6.1 +/- 1.8), Loefgren's syndrome, LS (11.9 +/- 2.6) and progressive sarcoidosis, PS (6.4 +/- 1.2, p < 0.05 for all), whereas the results in pneumoconioses were comparable to those obtained in controls (2.0 +/- 1.1 pg/ml, median +/- SEM). IFN-gamma results were positively correlated with total number of CD4+ cells (r(s) = +0.38, p < 0.05), CD4+ cells percentage (r(s) = +0.32, p < 0.005) and CD4+/CD8+ ratio (r(s) = +0.38, p = 0.0007), but negatively correlated with CD8+ cell percentage (r(s) = -0.39, p < 0.0005). In IPF patients with CD4/CD8 < or =1 (n=9) IFN-gamma level was lower as compared with the group with CD4/CD8 >1 (n=7), 2.8 +/- 1.3 vs. 7.3 +/- 1.0 pg/ml. In sarcoidosis, IFN-gamma level did not seem to have a prognostic role, since values obtained in PS did not differ remarkably from those in stable sarcoidosis and LS. Moreover, subsequent steroid treatment in 7 patients with progressive sarcoidosis did not change significantly IFN-gamma levels in BAL fluid.

CONCLUSIONS

Increased IFN-gamma level was found in non-treated patients with IPF, Loefgren's syndrome and progressive sarcoidosis. CD4+ (Th1), but neither CD8+ (Tc1) nor NK cells seem to be the main local source of IFN-gamma in ILD. Relatively low CD4/CD8 ratio in ILD may indicate the patients with increased risk of lung fibrosis.

Authors+Show Affiliations

Nicolaus Copernicus University in Toruń, Collegium Medicum of Bydgoszcz, Poland. mpkopins@consoft.com.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

pol

PubMed ID

18051824

Citation

Kopiński, Piotr, et al. "[Interferon Gamma (IFN-gamma) Level in Broncholaveolar Lavage (BAL) Fluid Is Positively Correlated With CD4/CD8 Ratio in Selected Interstitial Lung Diseases]." Polski Merkuriusz Lekarski : Organ Polskiego Towarzystwa Lekarskiego, vol. 23, no. 133, 2007, pp. 15-21.
Kopiński P, Przybylski G, Jarzemska A, et al. [Interferon gamma (IFN-gamma) level in broncholaveolar lavage (BAL) fluid is positively correlated with CD4/CD8 ratio in selected interstitial lung diseases]. Pol Merkur Lekarski. 2007;23(133):15-21.
Kopiński, P., Przybylski, G., Jarzemska, A., Sładek, K., Soja, J., Iwaniec, T., Balicka-Slusarczyk, B., Pinis, G., Dyczek, A., Szabłowska, K., Golińska, J., Jankowski, M., & Szczeklik, J. (2007). [Interferon gamma (IFN-gamma) level in broncholaveolar lavage (BAL) fluid is positively correlated with CD4/CD8 ratio in selected interstitial lung diseases]. Polski Merkuriusz Lekarski : Organ Polskiego Towarzystwa Lekarskiego, 23(133), 15-21.
Kopiński P, et al. [Interferon Gamma (IFN-gamma) Level in Broncholaveolar Lavage (BAL) Fluid Is Positively Correlated With CD4/CD8 Ratio in Selected Interstitial Lung Diseases]. Pol Merkur Lekarski. 2007;23(133):15-21. PubMed PMID: 18051824.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Interferon gamma (IFN-gamma) level in broncholaveolar lavage (BAL) fluid is positively correlated with CD4/CD8 ratio in selected interstitial lung diseases]. AU - Kopiński,Piotr, AU - Przybylski,Grzegorz, AU - Jarzemska,Agnieszka, AU - Sładek,Krzysztof, AU - Soja,Jerzy, AU - Iwaniec,Teresa, AU - Balicka-Slusarczyk,Barbara, AU - Pinis,Grazyna, AU - Dyczek,Andrzej, AU - Szabłowska,Karina, AU - Golińska,Joanna, AU - Jankowski,Marek, AU - Szczeklik,Jerzy, PY - 2007/12/7/pubmed PY - 2008/1/26/medline PY - 2007/12/7/entrez SP - 15 EP - 21 JF - Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego JO - Pol Merkur Lekarski VL - 23 IS - 133 N2 - UNLABELLED: IFN-gamma a potent antifibrotic activity in interstitial lung diseases (ILD). T cells, both Th1 and Tc1, are considered to be the main local source of IFN-gamma. MATERIAL AND METHODS: BAL fluids of 98 patients with ILD, incl. idiopathic pulmonary fibrosis (IPF/UIP), sarcoidosis, extrinsic allergic alveolitis (EAA), asbestosis and silicosis (n=16, 49, 7, 10, 16 resp.) were tested with ELISA for IFN-gamma levels. Results were compared with BAL cytoimmunology and patients' clinical data. RESULTS: Significantly increased IFN-gamma levels were found in non-treated patients with EAA (7.8 +/- 2.1), IPF (6.1 +/- 1.8), Loefgren's syndrome, LS (11.9 +/- 2.6) and progressive sarcoidosis, PS (6.4 +/- 1.2, p < 0.05 for all), whereas the results in pneumoconioses were comparable to those obtained in controls (2.0 +/- 1.1 pg/ml, median +/- SEM). IFN-gamma results were positively correlated with total number of CD4+ cells (r(s) = +0.38, p < 0.05), CD4+ cells percentage (r(s) = +0.32, p < 0.005) and CD4+/CD8+ ratio (r(s) = +0.38, p = 0.0007), but negatively correlated with CD8+ cell percentage (r(s) = -0.39, p < 0.0005). In IPF patients with CD4/CD8 < or =1 (n=9) IFN-gamma level was lower as compared with the group with CD4/CD8 >1 (n=7), 2.8 +/- 1.3 vs. 7.3 +/- 1.0 pg/ml. In sarcoidosis, IFN-gamma level did not seem to have a prognostic role, since values obtained in PS did not differ remarkably from those in stable sarcoidosis and LS. Moreover, subsequent steroid treatment in 7 patients with progressive sarcoidosis did not change significantly IFN-gamma levels in BAL fluid. CONCLUSIONS: Increased IFN-gamma level was found in non-treated patients with IPF, Loefgren's syndrome and progressive sarcoidosis. CD4+ (Th1), but neither CD8+ (Tc1) nor NK cells seem to be the main local source of IFN-gamma in ILD. Relatively low CD4/CD8 ratio in ILD may indicate the patients with increased risk of lung fibrosis. SN - 1426-9686 UR - https://www.unboundmedicine.com/medline/citation/18051824/[Interferon_gamma__IFN_gamma__level_in_broncholaveolar_lavage__BAL__fluid_is_positively_correlated_with_CD4/CD8_ratio_in_selected_interstitial_lung_diseases]_ L2 - https://medlineplus.gov/interstitiallungdiseases.html DB - PRIME DP - Unbound Medicine ER -