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Store-operated Ca2+ entry in astrocytes: different spatial arrangement of endoplasmic reticulum explains functional diversity in vitro and in situ.
Cell Calcium 2008; 43(6):591-601CC

Abstract

Ca(2+) signaling is the astrocyte form of excitability and the endoplasmic reticulum (ER) plays an important role as an intracellular Ca(2+) store. Since the subcellular distribution of the ER influences Ca(2+) signaling, we compared the arrangement of ER in astrocytes of hippocampus tissue and astrocytes in cell culture by electron microscopy. While the ER was usually located in close apposition to the plasma membrane in astrocytes in situ, the ER in cultured astrocytes was close to the nuclear membrane. Activation of metabotropic receptors linked to release of Ca(2+) from ER stores triggered distinct responses in cultured and in situ astrocytes. In culture, Ca(2+) signals were commonly first recorded close to the nucleus and with a delay at peripheral regions of the cells. Store-operated Ca(2+) entry (SOC) as a route to refill the Ca(2+) stores could be easily identified in cultured astrocytes as the Zn(2+)-sensitive component of the Ca(2+) signal. In contrast, such a Zn(2+)-sensitive component was not recorded in astrocytes from hippocampal slices despite of evidence for SOC. Our data indicate that both, astrocytes in situ and in vitro express SOC necessary to refill stores, but that a SOC-related signal is not recorded in the cytoplasm of astrocytes in situ since the stores are close to the plasma membrane and the refill does not affect cytoplasmic Ca(2+) levels.

Authors+Show Affiliations

Cytology Department, Bogomoletz Institute of Physiology, Bogomoletz Str. 4, 01024 Kiev, Ukraine.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18054077

Citation

Pivneva, Tatjyana, et al. "Store-operated Ca2+ Entry in Astrocytes: Different Spatial Arrangement of Endoplasmic Reticulum Explains Functional Diversity in Vitro and in Situ." Cell Calcium, vol. 43, no. 6, 2008, pp. 591-601.
Pivneva T, Haas B, Reyes-Haro D, et al. Store-operated Ca2+ entry in astrocytes: different spatial arrangement of endoplasmic reticulum explains functional diversity in vitro and in situ. Cell Calcium. 2008;43(6):591-601.
Pivneva, T., Haas, B., Reyes-Haro, D., Laube, G., Veh, R. W., Nolte, C., ... Kettenmann, H. (2008). Store-operated Ca2+ entry in astrocytes: different spatial arrangement of endoplasmic reticulum explains functional diversity in vitro and in situ. Cell Calcium, 43(6), pp. 591-601.
Pivneva T, et al. Store-operated Ca2+ Entry in Astrocytes: Different Spatial Arrangement of Endoplasmic Reticulum Explains Functional Diversity in Vitro and in Situ. Cell Calcium. 2008;43(6):591-601. PubMed PMID: 18054077.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Store-operated Ca2+ entry in astrocytes: different spatial arrangement of endoplasmic reticulum explains functional diversity in vitro and in situ. AU - Pivneva,Tatjyana, AU - Haas,Brigitte, AU - Reyes-Haro,Daniel, AU - Laube,Gregor, AU - Veh,Ruediger W, AU - Nolte,Christiane, AU - Skibo,Galina, AU - Kettenmann,Helmut, Y1 - 2007/12/03/ PY - 2007/07/06/received PY - 2007/09/19/revised PY - 2007/10/05/accepted PY - 2007/12/7/pubmed PY - 2008/9/6/medline PY - 2007/12/7/entrez SP - 591 EP - 601 JF - Cell calcium JO - Cell Calcium VL - 43 IS - 6 N2 - Ca(2+) signaling is the astrocyte form of excitability and the endoplasmic reticulum (ER) plays an important role as an intracellular Ca(2+) store. Since the subcellular distribution of the ER influences Ca(2+) signaling, we compared the arrangement of ER in astrocytes of hippocampus tissue and astrocytes in cell culture by electron microscopy. While the ER was usually located in close apposition to the plasma membrane in astrocytes in situ, the ER in cultured astrocytes was close to the nuclear membrane. Activation of metabotropic receptors linked to release of Ca(2+) from ER stores triggered distinct responses in cultured and in situ astrocytes. In culture, Ca(2+) signals were commonly first recorded close to the nucleus and with a delay at peripheral regions of the cells. Store-operated Ca(2+) entry (SOC) as a route to refill the Ca(2+) stores could be easily identified in cultured astrocytes as the Zn(2+)-sensitive component of the Ca(2+) signal. In contrast, such a Zn(2+)-sensitive component was not recorded in astrocytes from hippocampal slices despite of evidence for SOC. Our data indicate that both, astrocytes in situ and in vitro express SOC necessary to refill stores, but that a SOC-related signal is not recorded in the cytoplasm of astrocytes in situ since the stores are close to the plasma membrane and the refill does not affect cytoplasmic Ca(2+) levels. SN - 0143-4160 UR - https://www.unboundmedicine.com/medline/citation/18054077/Store_operated_Ca2+_entry_in_astrocytes:_different_spatial_arrangement_of_endoplasmic_reticulum_explains_functional_diversity_in_vitro_and_in_situ_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4160(07)00180-7 DB - PRIME DP - Unbound Medicine ER -