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Genotype analysis of enterotoxin H-positive Staphylococcus aureus strains isolated from food samples in the Czech Republic.
Int J Food Microbiol. 2008 Jan 15; 121(1):60-5.IJ

Abstract

Twenty-eight enterotoxin H-positive Staphylococcus aureus strains isolated from food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were genotypically characterized by pulsed-field gel electrophoresis (PFGE) profiling, spa gene polymorphism analysis, enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) fingerprinting and prophage carriage detection. These strains accounted for about 21% of the food-derived, staphylococcal enterotoxin (SE)-positive isolates. One strain, detected in feta cheese, was implicated in a case of enterotoxinosis. Sixteen of the twenty-eight isolates carried the seh gene alone. The remaining twelve strains harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Comparison of various genomic profiles resulted in the determination of twenty genotypes designated G-1 to G-20. Two new, to date not defined, spa types (t2000 and t2002) were identified in one strain isolated from raw meat and two strains obtained from prepacked pizza. Evidence has been given that the seh-positive S. aureus isolates from foodstuffs did not originate from a single source or a common ancestor.

Authors+Show Affiliations

Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Brno, Czech Republic. vladkar@sci.muni.czNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18054105

Citation

Růzicková, Vladislava, et al. "Genotype Analysis of Enterotoxin H-positive Staphylococcus Aureus Strains Isolated From Food Samples in the Czech Republic." International Journal of Food Microbiology, vol. 121, no. 1, 2008, pp. 60-5.
Růzicková V, Karpísková R, Pantůcek R, et al. Genotype analysis of enterotoxin H-positive Staphylococcus aureus strains isolated from food samples in the Czech Republic. Int J Food Microbiol. 2008;121(1):60-5.
Růzicková, V., Karpísková, R., Pantůcek, R., Pospísilová, M., Cerníková, P., & Doskar, J. (2008). Genotype analysis of enterotoxin H-positive Staphylococcus aureus strains isolated from food samples in the Czech Republic. International Journal of Food Microbiology, 121(1), 60-5.
Růzicková V, et al. Genotype Analysis of Enterotoxin H-positive Staphylococcus Aureus Strains Isolated From Food Samples in the Czech Republic. Int J Food Microbiol. 2008 Jan 15;121(1):60-5. PubMed PMID: 18054105.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotype analysis of enterotoxin H-positive Staphylococcus aureus strains isolated from food samples in the Czech Republic. AU - Růzicková,Vladislava, AU - Karpísková,Renata, AU - Pantůcek,Roman, AU - Pospísilová,Markéta, AU - Cerníková,Pavla, AU - Doskar,Jirí, Y1 - 2007/11/01/ PY - 2007/03/14/received PY - 2007/08/07/revised PY - 2007/10/24/accepted PY - 2007/12/7/pubmed PY - 2008/4/11/medline PY - 2007/12/7/entrez SP - 60 EP - 5 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 121 IS - 1 N2 - Twenty-eight enterotoxin H-positive Staphylococcus aureus strains isolated from food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were genotypically characterized by pulsed-field gel electrophoresis (PFGE) profiling, spa gene polymorphism analysis, enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) fingerprinting and prophage carriage detection. These strains accounted for about 21% of the food-derived, staphylococcal enterotoxin (SE)-positive isolates. One strain, detected in feta cheese, was implicated in a case of enterotoxinosis. Sixteen of the twenty-eight isolates carried the seh gene alone. The remaining twelve strains harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Comparison of various genomic profiles resulted in the determination of twenty genotypes designated G-1 to G-20. Two new, to date not defined, spa types (t2000 and t2002) were identified in one strain isolated from raw meat and two strains obtained from prepacked pizza. Evidence has been given that the seh-positive S. aureus isolates from foodstuffs did not originate from a single source or a common ancestor. SN - 0168-1605 UR - https://www.unboundmedicine.com/medline/citation/18054105/Genotype_analysis_of_enterotoxin_H_positive_Staphylococcus_aureus_strains_isolated_from_food_samples_in_the_Czech_Republic_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1605(07)00560-0 DB - PRIME DP - Unbound Medicine ER -