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Aptamer biosensor for protein detection using gold nanoparticles.
Anal Biochem. 2008 Feb 15; 373(2):213-9.AB

Abstract

Combining gold nanoparticles (GNPs) as fluorescence quencher and aptamer as probe, we have developed protein biosensors by using DNA-modified GNPs. We examined how the experimental design, such as the type of interaction between DNA strands and GNPs, temperature, and microenvironment of aptamer, influences the recognition ability of the biosensor. Under our experimental conditions, the recognition of protein by the complex of dye-labeled DNA hybridized with aptamer that is immobilized on GNPs (Ap-Im-GNPs) shows the best character in protein detection.

Authors+Show Affiliations

Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18054771

Citation

Wang, Wenjuan, et al. "Aptamer Biosensor for Protein Detection Using Gold Nanoparticles." Analytical Biochemistry, vol. 373, no. 2, 2008, pp. 213-9.
Wang W, Chen C, Qian M, et al. Aptamer biosensor for protein detection using gold nanoparticles. Anal Biochem. 2008;373(2):213-9.
Wang, W., Chen, C., Qian, M., & Zhao, X. S. (2008). Aptamer biosensor for protein detection using gold nanoparticles. Analytical Biochemistry, 373(2), 213-9.
Wang W, et al. Aptamer Biosensor for Protein Detection Using Gold Nanoparticles. Anal Biochem. 2008 Feb 15;373(2):213-9. PubMed PMID: 18054771.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aptamer biosensor for protein detection using gold nanoparticles. AU - Wang,Wenjuan, AU - Chen,Chunlai, AU - Qian,Minxie, AU - Zhao,Xin Sheng, Y1 - 2007/11/17/ PY - 2007/07/21/received PY - 2007/10/29/revised PY - 2007/11/03/accepted PY - 2007/12/7/pubmed PY - 2008/3/7/medline PY - 2007/12/7/entrez SP - 213 EP - 9 JF - Analytical biochemistry JO - Anal Biochem VL - 373 IS - 2 N2 - Combining gold nanoparticles (GNPs) as fluorescence quencher and aptamer as probe, we have developed protein biosensors by using DNA-modified GNPs. We examined how the experimental design, such as the type of interaction between DNA strands and GNPs, temperature, and microenvironment of aptamer, influences the recognition ability of the biosensor. Under our experimental conditions, the recognition of protein by the complex of dye-labeled DNA hybridized with aptamer that is immobilized on GNPs (Ap-Im-GNPs) shows the best character in protein detection. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/18054771/Aptamer_biosensor_for_protein_detection_using_gold_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(07)00724-5 DB - PRIME DP - Unbound Medicine ER -