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Genetic and functional properties of the self-transmissible Yersinia enterocolitica plasmid pYE854, which mobilizes the virulence plasmid pYV.
J Bacteriol 2008; 190(3):991-1010JB

Abstract

Yersinia strains frequently harbor plasmids, of which the virulence plasmid pYV, indigenous in pathogenic strains, has been thoroughly characterized during the last decades. Yet, it has been unknown whether the nonconjugative pYV can be transferred by helper plasmids naturally occurring in this genus. We have isolated the conjugative plasmids pYE854 (95.5 kb) and pYE966 (70 kb) from a nonpathogenic and a pathogenic Yersinia enterocolitica strain, respectively, and demonstrate that both plasmids are able to mobilize pYV. The complete sequence of pYE854 has been determined. The transfer proteins and oriT of the plasmid reveal similarities to the F factor. However, the pYE854 replicon does not belong to the IncF group and is more closely related to a plasmid of gram-positive bacteria. Plasmid pYE966 is very similar to pYE854 but lacks two DNA regions of the larger plasmid that are dispensable for conjugation.

Authors+Show Affiliations

Bundesinstitut für Risikobewertung, D-12277 Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18055592

Citation

Hammerl, Jens A., et al. "Genetic and Functional Properties of the Self-transmissible Yersinia Enterocolitica Plasmid pYE854, Which Mobilizes the Virulence Plasmid PYV." Journal of Bacteriology, vol. 190, no. 3, 2008, pp. 991-1010.
Hammerl JA, Klein I, Lanka E, et al. Genetic and functional properties of the self-transmissible Yersinia enterocolitica plasmid pYE854, which mobilizes the virulence plasmid pYV. J Bacteriol. 2008;190(3):991-1010.
Hammerl, J. A., Klein, I., Lanka, E., Appel, B., & Hertwig, S. (2008). Genetic and functional properties of the self-transmissible Yersinia enterocolitica plasmid pYE854, which mobilizes the virulence plasmid pYV. Journal of Bacteriology, 190(3), pp. 991-1010.
Hammerl JA, et al. Genetic and Functional Properties of the Self-transmissible Yersinia Enterocolitica Plasmid pYE854, Which Mobilizes the Virulence Plasmid PYV. J Bacteriol. 2008;190(3):991-1010. PubMed PMID: 18055592.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic and functional properties of the self-transmissible Yersinia enterocolitica plasmid pYE854, which mobilizes the virulence plasmid pYV. AU - Hammerl,Jens A, AU - Klein,Iris, AU - Lanka,Erich, AU - Appel,Bernd, AU - Hertwig,Stefan, Y1 - 2007/11/30/ PY - 2007/12/7/pubmed PY - 2008/2/13/medline PY - 2007/12/7/entrez SP - 991 EP - 1010 JF - Journal of bacteriology JO - J. Bacteriol. VL - 190 IS - 3 N2 - Yersinia strains frequently harbor plasmids, of which the virulence plasmid pYV, indigenous in pathogenic strains, has been thoroughly characterized during the last decades. Yet, it has been unknown whether the nonconjugative pYV can be transferred by helper plasmids naturally occurring in this genus. We have isolated the conjugative plasmids pYE854 (95.5 kb) and pYE966 (70 kb) from a nonpathogenic and a pathogenic Yersinia enterocolitica strain, respectively, and demonstrate that both plasmids are able to mobilize pYV. The complete sequence of pYE854 has been determined. The transfer proteins and oriT of the plasmid reveal similarities to the F factor. However, the pYE854 replicon does not belong to the IncF group and is more closely related to a plasmid of gram-positive bacteria. Plasmid pYE966 is very similar to pYE854 but lacks two DNA regions of the larger plasmid that are dispensable for conjugation. SN - 1098-5530 UR - https://www.unboundmedicine.com/medline/citation/18055592/Genetic_and_functional_properties_of_the_self_transmissible_Yersinia_enterocolitica_plasmid_pYE854_which_mobilizes_the_virulence_plasmid_pYV_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=18055592 DB - PRIME DP - Unbound Medicine ER -