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Advances in fluorescent protein technology.
J Cell Sci. 2007 Dec 15; 120(Pt 24):4247-60.JC

Abstract

Current fluorescent protein (FP) development strategies are focused on fine-tuning the photophysical properties of blue to yellow variants derived from the Aequorea victoria jellyfish green fluorescent protein (GFP) and on the development of monomeric FPs from other organisms that emit in the yellow-orange to far-red regions of the visible light spectrum. Progress toward these goals has been substantial, and near-infrared emitting FPs may loom over the horizon. The latest efforts in jellyfish variants have resulted in new and improved monomeric BFP, CFP, GFP and YFP variants, and the relentless search for a bright, monomeric and fast-maturing red FP has yielded a host of excellent candidates, although none is yet optimal for all applications. Meanwhile, photoactivatable FPs are emerging as a powerful class of probes for intracellular dynamics and, unexpectedly, as useful tools for the development of superresolution microscopy applications.

Authors+Show Affiliations

The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA. shaner@salk.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

18057027

Citation

Shaner, Nathan C., et al. "Advances in Fluorescent Protein Technology." Journal of Cell Science, vol. 120, no. Pt 24, 2007, pp. 4247-60.
Shaner NC, Patterson GH, Davidson MW. Advances in fluorescent protein technology. J Cell Sci. 2007;120(Pt 24):4247-60.
Shaner, N. C., Patterson, G. H., & Davidson, M. W. (2007). Advances in fluorescent protein technology. Journal of Cell Science, 120(Pt 24), 4247-60.
Shaner NC, Patterson GH, Davidson MW. Advances in Fluorescent Protein Technology. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60. PubMed PMID: 18057027.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Advances in fluorescent protein technology. AU - Shaner,Nathan C, AU - Patterson,George H, AU - Davidson,Michael W, PY - 2007/12/7/pubmed PY - 2008/3/18/medline PY - 2007/12/7/entrez SP - 4247 EP - 60 JF - Journal of cell science JO - J. Cell. Sci. VL - 120 IS - Pt 24 N2 - Current fluorescent protein (FP) development strategies are focused on fine-tuning the photophysical properties of blue to yellow variants derived from the Aequorea victoria jellyfish green fluorescent protein (GFP) and on the development of monomeric FPs from other organisms that emit in the yellow-orange to far-red regions of the visible light spectrum. Progress toward these goals has been substantial, and near-infrared emitting FPs may loom over the horizon. The latest efforts in jellyfish variants have resulted in new and improved monomeric BFP, CFP, GFP and YFP variants, and the relentless search for a bright, monomeric and fast-maturing red FP has yielded a host of excellent candidates, although none is yet optimal for all applications. Meanwhile, photoactivatable FPs are emerging as a powerful class of probes for intracellular dynamics and, unexpectedly, as useful tools for the development of superresolution microscopy applications. SN - 0021-9533 UR - https://www.unboundmedicine.com/medline/citation/18057027/Advances_in_fluorescent_protein_technology_ L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=18057027 DB - PRIME DP - Unbound Medicine ER -