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Rates of chilling to 0 degrees C: implications for the survival of microorganisms and relationship with membrane fluidity modifications.
Appl Microbiol Biotechnol. 2008 Jan; 77(6):1379-87.AM

Abstract

The effects of slow chilling (2 degrees C min(-1)) and rapid chilling (2,000 degrees C min(-1)) were investigated on the survival and membrane fluidity of Escherichia coli, of Bacillus subtilis, and of Saccharomyces cerevisiae. Cell death was found to be dependent on the physiological state of cell cultures and on the rate of temperature downshift. Slow temperature decrease allowed cell stabilization, whereas the rapid chilling induced an immediate loss of viability of up to more than 90 and 70% for the exponentially growing cells of E. coli and B. subtilis, respectively. To relate the results of viability with changes in membrane physical state, membrane anisotropy variation was monitored during thermal stress using the fluorescence probe 1,6-diphenyl-1,3,5-hexatriene. No variation in the membrane fluidity of all the three microorganisms was found after the slow chilling. It is interesting to note that fluorescence measurements showed an irreversible rigidification of the membrane of exponentially growing cells of E. coli and B. subtilis after the instantaneous cold shock, which was not observed with S. cerevisiae. This irreversible effect of the rapid cold shock on the membrane correlated well with high rates of cell inactivation. Thus, membrane alteration seems to be the principal cause of the cold shock injury.

Authors+Show Affiliations

Cao-Hoang L
Université de Bourgogne, 21000, Dijon, France.
Dumont F
No affiliation info available
Marechal PA
No affiliation info available
Le-Thanh M
No affiliation info available
Gervais P
No affiliation info available

MeSH

Bacillus subtilisCell MembraneCold TemperatureEscherichia coliMembrane FluiditySaccharomyces cerevisiaeTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18060401

Citation

Cao-Hoang, L, et al. "Rates of Chilling to 0 Degrees C: Implications for the Survival of Microorganisms and Relationship With Membrane Fluidity Modifications." Applied Microbiology and Biotechnology, vol. 77, no. 6, 2008, pp. 1379-87.
Cao-Hoang L, Dumont F, Marechal PA, et al. Rates of chilling to 0 degrees C: implications for the survival of microorganisms and relationship with membrane fluidity modifications. Appl Microbiol Biotechnol. 2008;77(6):1379-87.
Cao-Hoang, L., Dumont, F., Marechal, P. A., Le-Thanh, M., & Gervais, P. (2008). Rates of chilling to 0 degrees C: implications for the survival of microorganisms and relationship with membrane fluidity modifications. Applied Microbiology and Biotechnology, 77(6), 1379-87.
Cao-Hoang L, et al. Rates of Chilling to 0 Degrees C: Implications for the Survival of Microorganisms and Relationship With Membrane Fluidity Modifications. Appl Microbiol Biotechnol. 2008;77(6):1379-87. PubMed PMID: 18060401.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rates of chilling to 0 degrees C: implications for the survival of microorganisms and relationship with membrane fluidity modifications. AU - Cao-Hoang,L, AU - Dumont,F, AU - Marechal,P A, AU - Le-Thanh,M, AU - Gervais,P, Y1 - 2007/12/01/ PY - 2006/12/21/received PY - 2007/11/06/accepted PY - 2007/12/7/pubmed PY - 2008/5/23/medline PY - 2007/12/7/entrez SP - 1379 EP - 87 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 77 IS - 6 N2 - The effects of slow chilling (2 degrees C min(-1)) and rapid chilling (2,000 degrees C min(-1)) were investigated on the survival and membrane fluidity of Escherichia coli, of Bacillus subtilis, and of Saccharomyces cerevisiae. Cell death was found to be dependent on the physiological state of cell cultures and on the rate of temperature downshift. Slow temperature decrease allowed cell stabilization, whereas the rapid chilling induced an immediate loss of viability of up to more than 90 and 70% for the exponentially growing cells of E. coli and B. subtilis, respectively. To relate the results of viability with changes in membrane physical state, membrane anisotropy variation was monitored during thermal stress using the fluorescence probe 1,6-diphenyl-1,3,5-hexatriene. No variation in the membrane fluidity of all the three microorganisms was found after the slow chilling. It is interesting to note that fluorescence measurements showed an irreversible rigidification of the membrane of exponentially growing cells of E. coli and B. subtilis after the instantaneous cold shock, which was not observed with S. cerevisiae. This irreversible effect of the rapid cold shock on the membrane correlated well with high rates of cell inactivation. Thus, membrane alteration seems to be the principal cause of the cold shock injury. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/18060401/Rates_of_chilling_to_0_degrees_C:_implications_for_the_survival_of_microorganisms_and_relationship_with_membrane_fluidity_modifications_ DB - PRIME DP - Unbound Medicine ER -