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Features of ceruloplasmin in the cerebrospinal fluid of Alzheimer's disease patients.

Abstract

The level of the apo-form of the copper enzyme ceruloplasmin (CP) is an established peripheral marker in diseases associated with copper imbalance. In view of the proposal that disturbances of copper homeostasis may contribute to neurodegeneration associated with Alzheimer's disease (AD), the present work investigates, by Western blot and non-reducing SDS-PAGE followed by activity staining, the features of CP protein, and the copper/CP relationship in cerebrospinal fluid (CSF) and serum of AD patients. Results show that only a fraction of total copper is associated with CP in the CSF, at variance with serum, both in affected and in healthy individuals. Furthermore, a conspicuous amount of apo-ceruloplasmin and a decrease of CP oxidase activity characterize the CSF of the affected individuals, and confirm that an impairment of copper metabolism occurs in their central nervous system. In the CSF of AD patients the decrease of active CP, associated with the increase in the pool of copper not sequestered by this protein, may play a role in the neurodegenerative process.

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  • Authors+Show Affiliations

    ,

    Department of Biology, "Tor Vergata" University of Rome, Via della Ricerca Scientifica, 00133 Rome, Italy.

    , , , , ,

    Source

    MeSH

    Aged
    Alzheimer Disease
    Ceruloplasmin
    Female
    Holoenzymes
    Humans
    Male

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    18060472

    Citation

    Capo, Concetta R., et al. "Features of Ceruloplasmin in the Cerebrospinal Fluid of Alzheimer's Disease Patients." Biometals : an International Journal On the Role of Metal Ions in Biology, Biochemistry, and Medicine, vol. 21, no. 3, 2008, pp. 367-72.
    Capo CR, Arciello M, Squitti R, et al. Features of ceruloplasmin in the cerebrospinal fluid of Alzheimer's disease patients. Biometals. 2008;21(3):367-72.
    Capo, C. R., Arciello, M., Squitti, R., Cassetta, E., Rossini, P. M., Calabrese, L., & Rossi, L. (2008). Features of ceruloplasmin in the cerebrospinal fluid of Alzheimer's disease patients. Biometals : an International Journal On the Role of Metal Ions in Biology, Biochemistry, and Medicine, 21(3), pp. 367-72.
    Capo CR, et al. Features of Ceruloplasmin in the Cerebrospinal Fluid of Alzheimer's Disease Patients. Biometals. 2008;21(3):367-72. PubMed PMID: 18060472.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Features of ceruloplasmin in the cerebrospinal fluid of Alzheimer's disease patients. AU - Capo,Concetta R, AU - Arciello,Mario, AU - Squitti,Rosanna, AU - Cassetta,Emanuele, AU - Rossini,Paolo Maria, AU - Calabrese,Lilia, AU - Rossi,Luisa, Y1 - 2007/11/30/ PY - 2007/06/19/received PY - 2007/11/16/accepted PY - 2007/12/7/pubmed PY - 2008/8/7/medline PY - 2007/12/7/entrez SP - 367 EP - 72 JF - Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine JO - Biometals VL - 21 IS - 3 N2 - The level of the apo-form of the copper enzyme ceruloplasmin (CP) is an established peripheral marker in diseases associated with copper imbalance. In view of the proposal that disturbances of copper homeostasis may contribute to neurodegeneration associated with Alzheimer's disease (AD), the present work investigates, by Western blot and non-reducing SDS-PAGE followed by activity staining, the features of CP protein, and the copper/CP relationship in cerebrospinal fluid (CSF) and serum of AD patients. Results show that only a fraction of total copper is associated with CP in the CSF, at variance with serum, both in affected and in healthy individuals. Furthermore, a conspicuous amount of apo-ceruloplasmin and a decrease of CP oxidase activity characterize the CSF of the affected individuals, and confirm that an impairment of copper metabolism occurs in their central nervous system. In the CSF of AD patients the decrease of active CP, associated with the increase in the pool of copper not sequestered by this protein, may play a role in the neurodegenerative process. SN - 0966-0844 UR - https://www.unboundmedicine.com/medline/citation/18060472/Features_of_ceruloplasmin_in_the_cerebrospinal_fluid_of_Alzheimer's_disease_patients_ L2 - https://doi.org/10.1007/s10534-007-9125-4 DB - PRIME DP - Unbound Medicine ER -