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Effect of recombinant human TNF-binding protein-1 and GnRH antagonist on mRNA expression of inflammatory cytokines and adhesion and growth factors in endometrium and endometriosis tissues in baboons.
Fertil Steril. 2008 May; 89(5 Suppl):1306-13.FS

Abstract

OBJECTIVE

To evaluate the mechanism of action of recombinant human tumor necrosis factor (TNF)-binding protein-1 by assessing differential expression of messenger RNA (mRNA) for cytokines, matrix metalloproteinases, and growth and adhesion factors in baboons.

DESIGN

Analysis of gene expression in a prospective randomized study.

SETTING

University Fertility Center.

ANIMAL(S)

In the in vivo study, 14 baboons were randomly and subcutaneously (SC) treated with either phosphate-buffered saline (PBS), GnRH antagonist, or recombinant human TNF-binding protein-1 at the time of induction. In the ex vivo study, 4 baboons were treated by menstrual endometrium that had been incubated randomly with either PBS or recombinant human TNF-binding protein-1 before intrapelvic injection.

INTERVENTION(S)

In the in vivo study, analysis of 11 endometrial and 10 endometriosis biopsies included either PBS (n = 5), GnRH antagonist (n = 8), or recombinant human TNF-binding protein-1 (n = 8). In the ex vivo study, 2 endometrial and 4 endometriosis biopsies were analyzed from 4 baboons.

MAIN OUTCOME MEASURE(S)

The mRNA expression of TNF-alpha, IL-8, IL-6, transforming growth factor-beta (TGF-beta), vascular endothelial growth factor, intercellular adhesion molecule-1, matrix metalloproteinase-1, and regulated on activation, normal T-cell expressed and secreted were investigated using real-time reverse transcriptase-polymer chain reaction (PCR).

RESULT(S)

TGF-beta mRNA expression was decreased in endometriotic lesions from baboons treated with recombinant human TNF-binding protein-1 when compared with the placebo group.

CONCLUSION(S)

Except TGF-beta, mRNA expression of inflammatory cytokines and adhesion/growth factors is not affected in endometrial and endometriosis biopsies from baboons after induction of endometriosis combined with either systemic injection of recombinant human TNF-binding or GnRH antagonist or ex vivo treatment with recombinant human TNF-binding protein-1. Further studies are needed to elucidate the mode of action on how inhibition of TNF-alpha activity prevents the development of endometriosis.

Authors+Show Affiliations

Leuven University Fertility Centre, Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Leuven, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18061171

Citation

Kyama, Cleophas M., et al. "Effect of Recombinant Human TNF-binding Protein-1 and GnRH Antagonist On mRNA Expression of Inflammatory Cytokines and Adhesion and Growth Factors in Endometrium and Endometriosis Tissues in Baboons." Fertility and Sterility, vol. 89, no. 5 Suppl, 2008, pp. 1306-13.
Kyama CM, Overbergh L, Mihalyi A, et al. Effect of recombinant human TNF-binding protein-1 and GnRH antagonist on mRNA expression of inflammatory cytokines and adhesion and growth factors in endometrium and endometriosis tissues in baboons. Fertil Steril. 2008;89(5 Suppl):1306-13.
Kyama, C. M., Overbergh, L., Mihalyi, A., Cuneo, S., Chai, D., Debrock, S., Mwenda, J. M., Mathieu, C., Nugent, N. P., & D'Hooghe, T. M. (2008). Effect of recombinant human TNF-binding protein-1 and GnRH antagonist on mRNA expression of inflammatory cytokines and adhesion and growth factors in endometrium and endometriosis tissues in baboons. Fertility and Sterility, 89(5 Suppl), 1306-13.
Kyama CM, et al. Effect of Recombinant Human TNF-binding Protein-1 and GnRH Antagonist On mRNA Expression of Inflammatory Cytokines and Adhesion and Growth Factors in Endometrium and Endometriosis Tissues in Baboons. Fertil Steril. 2008;89(5 Suppl):1306-13. PubMed PMID: 18061171.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of recombinant human TNF-binding protein-1 and GnRH antagonist on mRNA expression of inflammatory cytokines and adhesion and growth factors in endometrium and endometriosis tissues in baboons. AU - Kyama,Cleophas M, AU - Overbergh,Lutgart, AU - Mihalyi,Attila, AU - Cuneo,Silvio, AU - Chai,Daniel, AU - Debrock,Sophie, AU - Mwenda,Jason M, AU - Mathieu,Chantal, AU - Nugent,Noreen P, AU - D'Hooghe,Thomas M, Y1 - 2007/12/03/ PY - 2006/07/04/received PY - 2006/11/21/revised PY - 2006/11/27/accepted PY - 2007/12/7/pubmed PY - 2008/7/17/medline PY - 2007/12/7/entrez SP - 1306 EP - 13 JF - Fertility and sterility JO - Fertil. Steril. VL - 89 IS - 5 Suppl N2 - OBJECTIVE: To evaluate the mechanism of action of recombinant human tumor necrosis factor (TNF)-binding protein-1 by assessing differential expression of messenger RNA (mRNA) for cytokines, matrix metalloproteinases, and growth and adhesion factors in baboons. DESIGN: Analysis of gene expression in a prospective randomized study. SETTING: University Fertility Center. ANIMAL(S): In the in vivo study, 14 baboons were randomly and subcutaneously (SC) treated with either phosphate-buffered saline (PBS), GnRH antagonist, or recombinant human TNF-binding protein-1 at the time of induction. In the ex vivo study, 4 baboons were treated by menstrual endometrium that had been incubated randomly with either PBS or recombinant human TNF-binding protein-1 before intrapelvic injection. INTERVENTION(S): In the in vivo study, analysis of 11 endometrial and 10 endometriosis biopsies included either PBS (n = 5), GnRH antagonist (n = 8), or recombinant human TNF-binding protein-1 (n = 8). In the ex vivo study, 2 endometrial and 4 endometriosis biopsies were analyzed from 4 baboons. MAIN OUTCOME MEASURE(S): The mRNA expression of TNF-alpha, IL-8, IL-6, transforming growth factor-beta (TGF-beta), vascular endothelial growth factor, intercellular adhesion molecule-1, matrix metalloproteinase-1, and regulated on activation, normal T-cell expressed and secreted were investigated using real-time reverse transcriptase-polymer chain reaction (PCR). RESULT(S): TGF-beta mRNA expression was decreased in endometriotic lesions from baboons treated with recombinant human TNF-binding protein-1 when compared with the placebo group. CONCLUSION(S): Except TGF-beta, mRNA expression of inflammatory cytokines and adhesion/growth factors is not affected in endometrial and endometriosis biopsies from baboons after induction of endometriosis combined with either systemic injection of recombinant human TNF-binding or GnRH antagonist or ex vivo treatment with recombinant human TNF-binding protein-1. Further studies are needed to elucidate the mode of action on how inhibition of TNF-alpha activity prevents the development of endometriosis. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/18061171/Effect_of_recombinant_human_TNF_binding_protein_1_and_GnRH_antagonist_on_mRNA_expression_of_inflammatory_cytokines_and_adhesion_and_growth_factors_in_endometrium_and_endometriosis_tissues_in_baboons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(07)00830-8 DB - PRIME DP - Unbound Medicine ER -