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Multiparametric analysis of normal and postchemotherapy bone marrow: Implication for the detection of leukemia-associated immunophenotypes.
Cytometry B Clin Cytom. 2008 Jan; 74(1):17-24.CB

Abstract

BACKGROUND

The knowledge of normal marrow is mandatory to assess the malignant counterpart of normal cells and define leukemia-associated immunophenotypes (LAIPs). In this study, the expression of a variety of antigens expressed in normal and postchemotherapy bone marrow (BM) was analyzed to provide a frame of reference for the identification of myeloid LAIPs.

METHODS

Multiparameter four- and six-color flow cytometry was used to define antigen combinations totally absent or present at very minimal levels in marrow cells of normal individuals (n = 20) and patients receiving chemotherapy for acute lymphoblastic leukemia (n = 20). Immature (blast) cells were gated according to CD45/SSC properties. Fifty-three acute myeloid leukemia (AML) samples were studied in six-color combinations.

RESULTS

In six-color flow cytometry, 47 phenotypes were totally absent from blast gate in all normal samples. Forty-one other phenotypes were identified in less than 0.05% of blast cells. There was no difference between normal and postchemotherapy BMs. The four-color panel allowed to identify only 30 phenotypes present at a frequency <0.05%. Using the six-color panel, 58% of the absent or infrequent phenotypes in normal BM were found in at least one of 53 AML samples. All AML cases exhibited at least one LAIP.

CONCLUSION

Our results show that the ability to distinguish leukemic from healthy cells is considerably increased by a six-color approach. Furthermore, these absent or infrequent phenotypes in normal BM are identified in AML and can be utilized for minimal residual disease study.

Authors+Show Affiliations

Laboratoire d'Hématologie Hôpital Nord, CHU de Saint-Etienne, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18061947

Citation

Olaru, D, et al. "Multiparametric Analysis of Normal and Postchemotherapy Bone Marrow: Implication for the Detection of Leukemia-associated Immunophenotypes." Cytometry. Part B, Clinical Cytometry, vol. 74, no. 1, 2008, pp. 17-24.
Olaru D, Campos L, Flandrin P, et al. Multiparametric analysis of normal and postchemotherapy bone marrow: Implication for the detection of leukemia-associated immunophenotypes. Cytometry B Clin Cytom. 2008;74(1):17-24.
Olaru, D., Campos, L., Flandrin, P., Nadal, N., Duval, A., Chautard, S., & Guyotat, D. (2008). Multiparametric analysis of normal and postchemotherapy bone marrow: Implication for the detection of leukemia-associated immunophenotypes. Cytometry. Part B, Clinical Cytometry, 74(1), 17-24.
Olaru D, et al. Multiparametric Analysis of Normal and Postchemotherapy Bone Marrow: Implication for the Detection of Leukemia-associated Immunophenotypes. Cytometry B Clin Cytom. 2008;74(1):17-24. PubMed PMID: 18061947.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiparametric analysis of normal and postchemotherapy bone marrow: Implication for the detection of leukemia-associated immunophenotypes. AU - Olaru,D, AU - Campos,L, AU - Flandrin,P, AU - Nadal,N, AU - Duval,A, AU - Chautard,S, AU - Guyotat,D, PY - 2007/12/7/pubmed PY - 2008/2/13/medline PY - 2007/12/7/entrez SP - 17 EP - 24 JF - Cytometry. Part B, Clinical cytometry JO - Cytometry B Clin Cytom VL - 74 IS - 1 N2 - BACKGROUND: The knowledge of normal marrow is mandatory to assess the malignant counterpart of normal cells and define leukemia-associated immunophenotypes (LAIPs). In this study, the expression of a variety of antigens expressed in normal and postchemotherapy bone marrow (BM) was analyzed to provide a frame of reference for the identification of myeloid LAIPs. METHODS: Multiparameter four- and six-color flow cytometry was used to define antigen combinations totally absent or present at very minimal levels in marrow cells of normal individuals (n = 20) and patients receiving chemotherapy for acute lymphoblastic leukemia (n = 20). Immature (blast) cells were gated according to CD45/SSC properties. Fifty-three acute myeloid leukemia (AML) samples were studied in six-color combinations. RESULTS: In six-color flow cytometry, 47 phenotypes were totally absent from blast gate in all normal samples. Forty-one other phenotypes were identified in less than 0.05% of blast cells. There was no difference between normal and postchemotherapy BMs. The four-color panel allowed to identify only 30 phenotypes present at a frequency <0.05%. Using the six-color panel, 58% of the absent or infrequent phenotypes in normal BM were found in at least one of 53 AML samples. All AML cases exhibited at least one LAIP. CONCLUSION: Our results show that the ability to distinguish leukemic from healthy cells is considerably increased by a six-color approach. Furthermore, these absent or infrequent phenotypes in normal BM are identified in AML and can be utilized for minimal residual disease study. SN - 1552-4957 UR - https://www.unboundmedicine.com/medline/citation/18061947/Multiparametric_analysis_of_normal_and_postchemotherapy_bone_marrow:_Implication_for_the_detection_of_leukemia_associated_immunophenotypes_ L2 - https://doi.org/10.1002/cyto.b.20371 DB - PRIME DP - Unbound Medicine ER -