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Effect of long-term selenium yeast intervention on activity and gene expression of antioxidant and xenobiotic metabolising enzymes in healthy elderly volunteers from the Danish Prevention of Cancer by Intervention by Selenium (PRECISE) pilot study.
Br J Nutr 2008; 99(6):1190-8BJ

Abstract

Numerous mechanisms have been proposed to explain the anti-carcinogenic effects of Se, among them altered carcinogen metabolism. We investigated the effect of Se supplementation on activities of glutathione peroxidase (GPX), glutathione reductase (GR) and glutathione S-transferase (GST) in different blood compartments, and expression of selected phase 1 and phase 2 genes in leucocytes (GPX1, gamma-glutamylcysteine ligase catalytic subunit (GCLC), AP-1 transcription factor Fos-related antigen 1 (Fra1), NAD(P)H:quinone oxidoreductase (NQO1), and aryl hydrocarbon receptor repressor (AhRR)). Healthy elderly Danes (n 105; age 71.3 (SD 4.26) years; 36% reporting use of multivitamin/mineral supplements) participated and were supplemented daily for 5 years with placebo, 100 microg, 200 microg or 300 microg Se as Se-enriched yeast (SelenoPrecise). Blood samples were collected after 5 years of intervention. When all four groups were compared we found no effect of Se supplementation on plasma GPX or GR, on erythrocyte GPX, GR or GST, or on thrombocyte GR or GST. We found increased thrombocyte GPX activity at the two highest dosage levels in women only, but not in men. No effects on GPX1, NQO1 or AhRR gene expression were found. When all Se-supplemented groups were pooled we found significant down regulation of the expression of some phase 2 genes (GCLC, Fra1). A significant increase in AhRR gene expression with smoking was found but was independent of Se supplementation. Down regulation of phase 2 genes could increase the risk of cancer. However, further studies are needed to establish whether the observed effect in leucocytes reflects a similar expression pattern in target tissues.

Authors+Show Affiliations

Department of Toxicology and Risk Assessment, National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, Søborg 2860, Denmark. grh@food.dtu.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18062829

Citation

Ravn-Haren, Gitte, et al. "Effect of Long-term Selenium Yeast Intervention On Activity and Gene Expression of Antioxidant and Xenobiotic Metabolising Enzymes in Healthy Elderly Volunteers From the Danish Prevention of Cancer By Intervention By Selenium (PRECISE) Pilot Study." The British Journal of Nutrition, vol. 99, no. 6, 2008, pp. 1190-8.
Ravn-Haren G, Krath BN, Overvad K, et al. Effect of long-term selenium yeast intervention on activity and gene expression of antioxidant and xenobiotic metabolising enzymes in healthy elderly volunteers from the Danish Prevention of Cancer by Intervention by Selenium (PRECISE) pilot study. Br J Nutr. 2008;99(6):1190-8.
Ravn-Haren, G., Krath, B. N., Overvad, K., Cold, S., Moesgaard, S., Larsen, E. H., & Dragsted, L. O. (2008). Effect of long-term selenium yeast intervention on activity and gene expression of antioxidant and xenobiotic metabolising enzymes in healthy elderly volunteers from the Danish Prevention of Cancer by Intervention by Selenium (PRECISE) pilot study. The British Journal of Nutrition, 99(6), pp. 1190-8.
Ravn-Haren G, et al. Effect of Long-term Selenium Yeast Intervention On Activity and Gene Expression of Antioxidant and Xenobiotic Metabolising Enzymes in Healthy Elderly Volunteers From the Danish Prevention of Cancer By Intervention By Selenium (PRECISE) Pilot Study. Br J Nutr. 2008;99(6):1190-8. PubMed PMID: 18062829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of long-term selenium yeast intervention on activity and gene expression of antioxidant and xenobiotic metabolising enzymes in healthy elderly volunteers from the Danish Prevention of Cancer by Intervention by Selenium (PRECISE) pilot study. AU - Ravn-Haren,Gitte, AU - Krath,Britta N, AU - Overvad,Kim, AU - Cold,Søren, AU - Moesgaard,Sven, AU - Larsen,Erik H, AU - Dragsted,Lars O, Y1 - 2007/12/06/ PY - 2007/12/8/pubmed PY - 2008/6/24/medline PY - 2007/12/8/entrez SP - 1190 EP - 8 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 99 IS - 6 N2 - Numerous mechanisms have been proposed to explain the anti-carcinogenic effects of Se, among them altered carcinogen metabolism. We investigated the effect of Se supplementation on activities of glutathione peroxidase (GPX), glutathione reductase (GR) and glutathione S-transferase (GST) in different blood compartments, and expression of selected phase 1 and phase 2 genes in leucocytes (GPX1, gamma-glutamylcysteine ligase catalytic subunit (GCLC), AP-1 transcription factor Fos-related antigen 1 (Fra1), NAD(P)H:quinone oxidoreductase (NQO1), and aryl hydrocarbon receptor repressor (AhRR)). Healthy elderly Danes (n 105; age 71.3 (SD 4.26) years; 36% reporting use of multivitamin/mineral supplements) participated and were supplemented daily for 5 years with placebo, 100 microg, 200 microg or 300 microg Se as Se-enriched yeast (SelenoPrecise). Blood samples were collected after 5 years of intervention. When all four groups were compared we found no effect of Se supplementation on plasma GPX or GR, on erythrocyte GPX, GR or GST, or on thrombocyte GR or GST. We found increased thrombocyte GPX activity at the two highest dosage levels in women only, but not in men. No effects on GPX1, NQO1 or AhRR gene expression were found. When all Se-supplemented groups were pooled we found significant down regulation of the expression of some phase 2 genes (GCLC, Fra1). A significant increase in AhRR gene expression with smoking was found but was independent of Se supplementation. Down regulation of phase 2 genes could increase the risk of cancer. However, further studies are needed to establish whether the observed effect in leucocytes reflects a similar expression pattern in target tissues. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/18062829/Effect_of_long_term_selenium_yeast_intervention_on_activity_and_gene_expression_of_antioxidant_and_xenobiotic_metabolising_enzymes_in_healthy_elderly_volunteers_from_the_Danish_Prevention_of_Cancer_by_Intervention_by_Selenium__PRECISE__pilot_study_ L2 - https://www.cambridge.org/core/product/identifier/S0007114507882948/type/journal_article DB - PRIME DP - Unbound Medicine ER -