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Distinct role of clathrin-mediated endocytosis in the functional uptake of cholera toxin.
Acta Biochim Pol. 2007; 54(4):757-67.AB

Abstract

The involvement of the clathrin-mediated endocytic internalization route in the uptake of cholera toxin (CT) was investigated using different cell lines, including the human intestinal Caco-2 and T84 cell lines, green monkey Vero cells, SH-SY5Y neuroblastoma cells and Madin-Darby canine kidney cells. Suppression of the clathrin-mediated endocytic pathway by classical biochemical procedures, like intracellular acidification and potassium depletion, inhibited cholera toxin uptake by up to about 50% as well as its ability to raise intracellular levels of cAMP. Also prior exposure of these cell types to the cationic amphiphilic drug chlorpromazine reduced the functional uptake of cholera toxin, even to a greater extent. These effects were dose- and cell type-dependent, suggesting an involvement of clathrin-mediated endocytosis in the functional uptake of cholera toxin. For a more straightforward approach to study the role of the clathrin-mediated uptake in the internalization of cholera toxin, a Caco-2(eps-) cell line was exploited. These Caco-2(eps-) cells constitutively suppress the expression of epsin, an essential accessory protein of clathrin-mediated endocytosis, thereby selectively blocking this internalization route. CT uptake was found to be reduced by over 60% in Caco-2(eps-) paralleled by a diminished ability of CT to raise the level of cAMP. The data presented suggest that the clathrin-mediated uptake route fulfils an important role in the functional internalization of cholera toxin in several cell types.

Authors+Show Affiliations

UA-Laboratory of Human Biochemistry, University of Antwerp, Antwerp, Belgium. davy.vandenbroeck@ua.ac.beNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18066406

Citation

Broeck, Davy Vanden, et al. "Distinct Role of Clathrin-mediated Endocytosis in the Functional Uptake of Cholera Toxin." Acta Biochimica Polonica, vol. 54, no. 4, 2007, pp. 757-67.
Broeck DV, Lagrou AR, De Wolf MJ. Distinct role of clathrin-mediated endocytosis in the functional uptake of cholera toxin. Acta Biochim Pol. 2007;54(4):757-67.
Broeck, D. V., Lagrou, A. R., & De Wolf, M. J. (2007). Distinct role of clathrin-mediated endocytosis in the functional uptake of cholera toxin. Acta Biochimica Polonica, 54(4), 757-67.
Broeck DV, Lagrou AR, De Wolf MJ. Distinct Role of Clathrin-mediated Endocytosis in the Functional Uptake of Cholera Toxin. Acta Biochim Pol. 2007;54(4):757-67. PubMed PMID: 18066406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distinct role of clathrin-mediated endocytosis in the functional uptake of cholera toxin. AU - Broeck,Davy Vanden, AU - Lagrou,Albert R, AU - De Wolf,Marc Js, Y1 - 2007/12/10/ PY - 2007/08/23/received PY - 2007/11/20/revised PY - 2007/12/03/accepted PY - 2007/12/11/pubmed PY - 2008/8/22/medline PY - 2007/12/11/entrez SP - 757 EP - 67 JF - Acta biochimica Polonica JO - Acta Biochim. Pol. VL - 54 IS - 4 N2 - The involvement of the clathrin-mediated endocytic internalization route in the uptake of cholera toxin (CT) was investigated using different cell lines, including the human intestinal Caco-2 and T84 cell lines, green monkey Vero cells, SH-SY5Y neuroblastoma cells and Madin-Darby canine kidney cells. Suppression of the clathrin-mediated endocytic pathway by classical biochemical procedures, like intracellular acidification and potassium depletion, inhibited cholera toxin uptake by up to about 50% as well as its ability to raise intracellular levels of cAMP. Also prior exposure of these cell types to the cationic amphiphilic drug chlorpromazine reduced the functional uptake of cholera toxin, even to a greater extent. These effects were dose- and cell type-dependent, suggesting an involvement of clathrin-mediated endocytosis in the functional uptake of cholera toxin. For a more straightforward approach to study the role of the clathrin-mediated uptake in the internalization of cholera toxin, a Caco-2(eps-) cell line was exploited. These Caco-2(eps-) cells constitutively suppress the expression of epsin, an essential accessory protein of clathrin-mediated endocytosis, thereby selectively blocking this internalization route. CT uptake was found to be reduced by over 60% in Caco-2(eps-) paralleled by a diminished ability of CT to raise the level of cAMP. The data presented suggest that the clathrin-mediated uptake route fulfils an important role in the functional internalization of cholera toxin in several cell types. SN - 0001-527X UR - https://www.unboundmedicine.com/medline/citation/18066406/Distinct_role_of_clathrin_mediated_endocytosis_in_the_functional_uptake_of_cholera_toxin_ L2 - http://www.actabp.pl/pdf/4_2007/757.pdf DB - PRIME DP - Unbound Medicine ER -