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Holotranscobalamin in laboratory diagnosis of cobalamin deficiency compared to total cobalamin and methylmalonic acid.
Clin Chem Lab Med. 2007; 45(12):1746-50.CC

Abstract

BACKGROUND

Cobalamin-saturated transcobalamin, also called holotranscobalamin (holoTC), constitutes only between 6% and 20% of total plasma B(12). Serum concentration of holoTC is a new marker in laboratory diagnosis of cobalamin deficiency. We tested the utility of holoTC in assessing vitamin B(12) status.

METHODS

We measured concentrations of holoTC and methylmalonic acid (MMA) in 1018 serum samples that were referred to our laboratory for total cobalamin testing.

RESULTS

Concentrations of MMA were lower in females compared to males and this difference was no more significant after adjusting for serum creatinine. Moreover, age was associated with higher concentrations of serum MMA, higher holoTC and slightly higher concentrations of total cobalamin. Higher concentrations of serum creatinine were associated with higher concentrations of MMA and holoTC. However, no association between serum creatinine and total cobalamin was observed. Only subjects with normal serum creatinine showed a negative correlation between serum holoTC and MMA (r= -0.36, p<0.001). In subjects with MMA > or =300 nmol/L and holoTC < or =35 pmol/L, concentrations of total cobalamin were well within the normal range (median; 25th/75th percentiles=212; 171/272 pmol/L). Receiver operating characteristic (ROC) curve analysis displayed a higher sensitivity and specificity for holoTC compared with vitamin B(12) for detecting concentrations of MMA > or =300 nmol/L in individuals with normal renal function.

CONCLUSIONS

Compared to total cobalamin, we observed a better performance of holoTC assay in detecting elevated concentrations of MMA in subjects with normal renal function. The majority of subjects with combined low holoTC and elevated MMA had normal concentrations of total cobalamin. HoloTC can be used as a first line parameter in detecting cobalamin deficiency.

Authors+Show Affiliations

Obeid R
Department of Clinical Chemistry and Laboratory Medicine, Faculty of Medicine, University Hospital of Saarland, Homburg/Saar, Germany.
Herrmann W
No affiliation info available

MeSH

AdultAgedAged, 80 and overFemaleHumansMaleMethylmalonic AcidMiddle AgedTranscobalaminsVitamin B 12Vitamin B 12 Deficiency

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

18067452

Citation

Obeid, Rima, and Wolfgang Herrmann. "Holotranscobalamin in Laboratory Diagnosis of Cobalamin Deficiency Compared to Total Cobalamin and Methylmalonic Acid." Clinical Chemistry and Laboratory Medicine, vol. 45, no. 12, 2007, pp. 1746-50.
Obeid R, Herrmann W. Holotranscobalamin in laboratory diagnosis of cobalamin deficiency compared to total cobalamin and methylmalonic acid. Clin Chem Lab Med. 2007;45(12):1746-50.
Obeid, R., & Herrmann, W. (2007). Holotranscobalamin in laboratory diagnosis of cobalamin deficiency compared to total cobalamin and methylmalonic acid. Clinical Chemistry and Laboratory Medicine, 45(12), 1746-50.
Obeid R, Herrmann W. Holotranscobalamin in Laboratory Diagnosis of Cobalamin Deficiency Compared to Total Cobalamin and Methylmalonic Acid. Clin Chem Lab Med. 2007;45(12):1746-50. PubMed PMID: 18067452.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Holotranscobalamin in laboratory diagnosis of cobalamin deficiency compared to total cobalamin and methylmalonic acid. AU - Obeid,Rima, AU - Herrmann,Wolfgang, PY - 2007/12/11/pubmed PY - 2008/4/2/medline PY - 2007/12/11/entrez SP - 1746 EP - 50 JF - Clinical chemistry and laboratory medicine JO - Clin Chem Lab Med VL - 45 IS - 12 N2 - BACKGROUND: Cobalamin-saturated transcobalamin, also called holotranscobalamin (holoTC), constitutes only between 6% and 20% of total plasma B(12). Serum concentration of holoTC is a new marker in laboratory diagnosis of cobalamin deficiency. We tested the utility of holoTC in assessing vitamin B(12) status. METHODS: We measured concentrations of holoTC and methylmalonic acid (MMA) in 1018 serum samples that were referred to our laboratory for total cobalamin testing. RESULTS: Concentrations of MMA were lower in females compared to males and this difference was no more significant after adjusting for serum creatinine. Moreover, age was associated with higher concentrations of serum MMA, higher holoTC and slightly higher concentrations of total cobalamin. Higher concentrations of serum creatinine were associated with higher concentrations of MMA and holoTC. However, no association between serum creatinine and total cobalamin was observed. Only subjects with normal serum creatinine showed a negative correlation between serum holoTC and MMA (r= -0.36, p<0.001). In subjects with MMA > or =300 nmol/L and holoTC < or =35 pmol/L, concentrations of total cobalamin were well within the normal range (median; 25th/75th percentiles=212; 171/272 pmol/L). Receiver operating characteristic (ROC) curve analysis displayed a higher sensitivity and specificity for holoTC compared with vitamin B(12) for detecting concentrations of MMA > or =300 nmol/L in individuals with normal renal function. CONCLUSIONS: Compared to total cobalamin, we observed a better performance of holoTC assay in detecting elevated concentrations of MMA in subjects with normal renal function. The majority of subjects with combined low holoTC and elevated MMA had normal concentrations of total cobalamin. HoloTC can be used as a first line parameter in detecting cobalamin deficiency. SN - 1434-6621 UR - https://www.unboundmedicine.com/medline/citation/18067452/Holotranscobalamin_in_laboratory_diagnosis_of_cobalamin_deficiency_compared_to_total_cobalamin_and_methylmalonic_acid_ DB - PRIME DP - Unbound Medicine ER -