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[Protection of liver against ischemia/reperfusion injury by Kupffer cell mediated emulsified isoflurane preconditioning: experiment with rats].
Zhonghua Yi Xue Za Zhi. 2007 Sep 18; 87(35):2468-71.ZY

Abstract

OBJECTIVE

To investigate the protective effect of pretreatment with Kupffer cell mediated emulsified isoflurane on ischemia/reperfusion injury in liver.

METHODS

Thirty-two SD rats underwent partial obstruction of liver blood flow for 30 m and were randomly divided into 4 equal groups during the operation: lipid emulsion control group (Group C, pretreated with intravenous injection of lipid emulsion), inhibition of Kupffer cell and preconditioning with lipid emulsion control group (Group KC, intraperitoneally injected with gadolinium chloride, inhibitor of Kupffer cells, and then pretreated with intravenous injection of lipid emulsion), preconditioning with emulsified isoflurane group (Group IP, intravenously injected with emulsified isoflurane), and Inhibition of Kupffer cells and preconditioning with isoflurane group (Group IK, intraperitoneally injected with gadolinium chloride and then intravenously injected with emulsified isoflurane), Then liver perfusion was recovered for 2 h and the rats were killed. Blood samples were collected from the vena cava to detect the serum alanine transaminase (ALT) and aspartate transaminase (AST). Specimens of left liver tissue were collected to undergo light microscopy. The contents of malonyldialdehyde (MDA) and superoxide dismutase (SOD) in the liver homogenate were examined with relevant kits.

RESULTS

Compared with Group C, the serum ALT and AST of Group IP were significantly lower (both P < 0.05). The MDA level of Group IP was significantly lower than that of Group C, and the SOD level of Group IP was significantly higher than that of Group C (both P < 0.05). However, there were no significant differences in the serum levels of ALT and AST and levels of MDA and SOD in lever homogenate among Group KC, IK, and C. The pathological changes in liver were remarkably milder in Group IP then in Group C, however, there were no significant differences in the pathological changes among Groups KC, IK, and C.

CONCLUSION

Preconditioning with emulsified isoflurane protects the liver from ischemia/reperfusion injury and this effect me be mediated by Kupffer cells.

Authors+Show Affiliations

Lü H
Department of Anesthesiology, Eastern Hepatobiliary surgery Hospital, Second Military medical University, Shanghai, China.
Yang LQ
No affiliation info available
Yu WF
No affiliation info available
Li Q
No affiliation info available
Ren HM
No affiliation info available
Zhu M
No affiliation info available
Xu LY
No affiliation info available

MeSH

Alanine TransaminaseAnimalsAspartate AminotransferasesDisease Models, AnimalEmulsionsInjections, IntravenousIschemic PreconditioningIsofluraneKupffer CellsLiverLiver DiseasesMaleMalondialdehydeRandom AllocationRatsRats, Sprague-DawleyReperfusion InjurySuperoxide Dismutase

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

18067806

Citation

Lü, Hao, et al. "[Protection of Liver Against Ischemia/reperfusion Injury By Kupffer Cell Mediated Emulsified Isoflurane Preconditioning: Experiment With Rats]." Zhonghua Yi Xue Za Zhi, vol. 87, no. 35, 2007, pp. 2468-71.
Lü H, Yang LQ, Yu WF, et al. [Protection of liver against ischemia/reperfusion injury by Kupffer cell mediated emulsified isoflurane preconditioning: experiment with rats]. Zhonghua Yi Xue Za Zhi. 2007;87(35):2468-71.
Lü, H., Yang, L. Q., Yu, W. F., Li, Q., Ren, H. M., Zhu, M., & Xu, L. Y. (2007). [Protection of liver against ischemia/reperfusion injury by Kupffer cell mediated emulsified isoflurane preconditioning: experiment with rats]. Zhonghua Yi Xue Za Zhi, 87(35), 2468-71.
Lü H, et al. [Protection of Liver Against Ischemia/reperfusion Injury By Kupffer Cell Mediated Emulsified Isoflurane Preconditioning: Experiment With Rats]. Zhonghua Yi Xue Za Zhi. 2007 Sep 18;87(35):2468-71. PubMed PMID: 18067806.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Protection of liver against ischemia/reperfusion injury by Kupffer cell mediated emulsified isoflurane preconditioning: experiment with rats]. AU - Lü,Hao, AU - Yang,Li-Qun, AU - Yu,Wei-Feng, AU - Li,Quan, AU - Ren,Hong-Mei, AU - Zhu,Min, AU - Xu,Li-Ya, PY - 2007/12/11/pubmed PY - 2008/7/1/medline PY - 2007/12/11/entrez SP - 2468 EP - 71 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 87 IS - 35 N2 - OBJECTIVE: To investigate the protective effect of pretreatment with Kupffer cell mediated emulsified isoflurane on ischemia/reperfusion injury in liver. METHODS: Thirty-two SD rats underwent partial obstruction of liver blood flow for 30 m and were randomly divided into 4 equal groups during the operation: lipid emulsion control group (Group C, pretreated with intravenous injection of lipid emulsion), inhibition of Kupffer cell and preconditioning with lipid emulsion control group (Group KC, intraperitoneally injected with gadolinium chloride, inhibitor of Kupffer cells, and then pretreated with intravenous injection of lipid emulsion), preconditioning with emulsified isoflurane group (Group IP, intravenously injected with emulsified isoflurane), and Inhibition of Kupffer cells and preconditioning with isoflurane group (Group IK, intraperitoneally injected with gadolinium chloride and then intravenously injected with emulsified isoflurane), Then liver perfusion was recovered for 2 h and the rats were killed. Blood samples were collected from the vena cava to detect the serum alanine transaminase (ALT) and aspartate transaminase (AST). Specimens of left liver tissue were collected to undergo light microscopy. The contents of malonyldialdehyde (MDA) and superoxide dismutase (SOD) in the liver homogenate were examined with relevant kits. RESULTS: Compared with Group C, the serum ALT and AST of Group IP were significantly lower (both P < 0.05). The MDA level of Group IP was significantly lower than that of Group C, and the SOD level of Group IP was significantly higher than that of Group C (both P < 0.05). However, there were no significant differences in the serum levels of ALT and AST and levels of MDA and SOD in lever homogenate among Group KC, IK, and C. The pathological changes in liver were remarkably milder in Group IP then in Group C, however, there were no significant differences in the pathological changes among Groups KC, IK, and C. CONCLUSION: Preconditioning with emulsified isoflurane protects the liver from ischemia/reperfusion injury and this effect me be mediated by Kupffer cells. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/18067806/[Protection_of_liver_against_ischemia/reperfusion_injury_by_Kupffer_cell_mediated_emulsified_isoflurane_preconditioning:_experiment_with_rats]_ DB - PRIME DP - Unbound Medicine ER -