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SERS detection of thrombin by protein recognition using functionalized gold nanoparticles.
Nanomedicine. 2007 Dec; 3(4):306-10.N

Abstract

We present a method based on surface-enhanced Raman spectroscopy and exploiting a protein-protein recognition process able to detect thrombin at subpicomolar concentrations. Gold nanoparticles (NPs) were capped with a bifunctional molecule capable of forming a covalent link with the aromatic residues of the protein moiety. The typical vibrations of the diazo bond established between the bifunctional molecule and the target protein are found to be selectively enhanced by the conjugated gold NPs, and therefore constitutes the Raman marker. After the interaction of functionalized NPs with antithrombin as a sensitive recognition element, immobilized on a capture substrate, we have detected thrombin at a concentration of about 10(-13) M.

Authors+Show Affiliations

Bizzarri AR
Biophysics and Nanoscience Centre, CNISM, Facolta' di Scienze, Università della Tuscia, Viterbo, Italy. bizzarri@unitus.it
Cannistraro S
No affiliation info available

MeSH

Biosensing TechniquesGoldNanoparticlesParticle SizeProtein Interaction MappingSpectrum Analysis, RamanSurface PropertiesThrombin

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18068092

Citation

Bizzarri, Anna Rita, and Salvatore Cannistraro. "SERS Detection of Thrombin By Protein Recognition Using Functionalized Gold Nanoparticles." Nanomedicine : Nanotechnology, Biology, and Medicine, vol. 3, no. 4, 2007, pp. 306-10.
Bizzarri AR, Cannistraro S. SERS detection of thrombin by protein recognition using functionalized gold nanoparticles. Nanomedicine. 2007;3(4):306-10.
Bizzarri, A. R., & Cannistraro, S. (2007). SERS detection of thrombin by protein recognition using functionalized gold nanoparticles. Nanomedicine : Nanotechnology, Biology, and Medicine, 3(4), 306-10.
Bizzarri AR, Cannistraro S. SERS Detection of Thrombin By Protein Recognition Using Functionalized Gold Nanoparticles. Nanomedicine. 2007;3(4):306-10. PubMed PMID: 18068092.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SERS detection of thrombin by protein recognition using functionalized gold nanoparticles. AU - Bizzarri,Anna Rita, AU - Cannistraro,Salvatore, PY - 2007/06/08/received PY - 2007/09/03/revised PY - 2007/09/24/accepted PY - 2007/12/11/pubmed PY - 2008/1/5/medline PY - 2007/12/11/entrez SP - 306 EP - 10 JF - Nanomedicine : nanotechnology, biology, and medicine JO - Nanomedicine VL - 3 IS - 4 N2 - We present a method based on surface-enhanced Raman spectroscopy and exploiting a protein-protein recognition process able to detect thrombin at subpicomolar concentrations. Gold nanoparticles (NPs) were capped with a bifunctional molecule capable of forming a covalent link with the aromatic residues of the protein moiety. The typical vibrations of the diazo bond established between the bifunctional molecule and the target protein are found to be selectively enhanced by the conjugated gold NPs, and therefore constitutes the Raman marker. After the interaction of functionalized NPs with antithrombin as a sensitive recognition element, immobilized on a capture substrate, we have detected thrombin at a concentration of about 10(-13) M. SN - 1549-9642 UR - https://www.unboundmedicine.com/medline/citation/18068092/SERS_detection_of_thrombin_by_protein_recognition_using_functionalized_gold_nanoparticles_ DB - PRIME DP - Unbound Medicine ER -