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Anti-tumor metastatic activity of beta-glucan purified from mutated Saccharomyces cerevisiae.
Int Immunopharmacol. 2008 Jan; 8(1):36-42.II

Abstract

The beta-glucans isolated from Saccharomyces cerevisiae (S. cerevisiae) enhance the innate immune system, but there is little evidence for its antitumor activity. To examine the antitumor and immunostimulating activities of beta-glucan (IS-2) purified from mutated S. cerevisiae, we made an experiment on innate immune response against metastasis of cancer cells by comparing with the beta-glucan from wild-type S. cerevisiae. In experimental lung metastasis of colon 26-M3.1 carcinoma or B16-BL6 melanoma cells, prophylactic administration of beta-glucan purified from mutated S. cerevisiae significantly inhibited lung metastasis in a dose-dependent manner. Furthermore, therapeutic administration of IS-2 also significantly inhibited the colon 26-M3.1 cell growth in mice. In an assay of liver and spleen metastasis produced by i.v. inoculation of L5178Y-ML25 lymphoma cells, IS-2 also significantly inhibited metastasis in CDF1 mice. Furthermore, pretreatment with IS-2 two days before tumor inoculation significantly prolonged the survival time of tumor-bearing mice. In an in vitro cytotoxicity analysis, IS-2 (up to 100 microg/ml) did not affect the growth of colon 26-M3.1 cells. In contrast, IS-2 enhanced splenocyte proliferating activity in a dose-dependent manner. Peritoneal macrophages stimulated with IS-2 produced various cytokines, such as IL-1beta, IFN-gamma, and IL-12. In addition, treatment with IS-2 (20 microg/mouse) induced tumoricidal activity of peritoneal macrophages against colon 26-M3.1 cells. In an assay for natural killer (NK) cell activity, IS-2 (20 microg/mouse, i.v.) significantly augmented NK cytotoxicity against Yac-1 tumor cells at 2 days after IS-2 treatment. The depletion of NK cells by injection of rabbit anti-asialo GM1 serum abolished the inhibitory effect of IS-2 on lung metastasis of colon 26-M3.1 cells. These data suggest that IS-2 inhibits tumor metastasis via activation of macrophages and NK cells.

Authors+Show Affiliations

Yoon TJ
Bio-Food and Drug Research Center and Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Republic of Korea.
Kim TJ
No affiliation info available
Lee H
No affiliation info available
Shin KS
No affiliation info available
Yun YP
No affiliation info available
Moon WK
No affiliation info available
Kim DW
No affiliation info available
Lee KH
No affiliation info available

MeSH

AnimalsCell Line, TumorCoculture TechniquesFemaleLeukemia L5178Lung NeoplasmsMelanoma, ExperimentalMiceMice, Inbred BALB CMice, Inbred C57BLMutagenesisRabbitsSaccharomyces cerevisiaebeta-Glucans

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18068098

Citation

Yoon, Taek Joon, et al. "Anti-tumor Metastatic Activity of Beta-glucan Purified From Mutated Saccharomyces Cerevisiae." International Immunopharmacology, vol. 8, no. 1, 2008, pp. 36-42.
Yoon TJ, Kim TJ, Lee H, et al. Anti-tumor metastatic activity of beta-glucan purified from mutated Saccharomyces cerevisiae. Int Immunopharmacol. 2008;8(1):36-42.
Yoon, T. J., Kim, T. J., Lee, H., Shin, K. S., Yun, Y. P., Moon, W. K., Kim, D. W., & Lee, K. H. (2008). Anti-tumor metastatic activity of beta-glucan purified from mutated Saccharomyces cerevisiae. International Immunopharmacology, 8(1), 36-42.
Yoon TJ, et al. Anti-tumor Metastatic Activity of Beta-glucan Purified From Mutated Saccharomyces Cerevisiae. Int Immunopharmacol. 2008;8(1):36-42. PubMed PMID: 18068098.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-tumor metastatic activity of beta-glucan purified from mutated Saccharomyces cerevisiae. AU - Yoon,Taek Joon, AU - Kim,Tack Joong, AU - Lee,Hwa, AU - Shin,Kwang Soon, AU - Yun,Yeo Pyo, AU - Moon,Won Kook, AU - Kim,Dong Woo, AU - Lee,Kwang Ho, Y1 - 2007/10/30/ PY - 2007/08/08/received PY - 2007/09/17/revised PY - 2007/10/04/accepted PY - 2007/12/11/pubmed PY - 2008/2/15/medline PY - 2007/12/11/entrez SP - 36 EP - 42 JF - International immunopharmacology JO - Int Immunopharmacol VL - 8 IS - 1 N2 - The beta-glucans isolated from Saccharomyces cerevisiae (S. cerevisiae) enhance the innate immune system, but there is little evidence for its antitumor activity. To examine the antitumor and immunostimulating activities of beta-glucan (IS-2) purified from mutated S. cerevisiae, we made an experiment on innate immune response against metastasis of cancer cells by comparing with the beta-glucan from wild-type S. cerevisiae. In experimental lung metastasis of colon 26-M3.1 carcinoma or B16-BL6 melanoma cells, prophylactic administration of beta-glucan purified from mutated S. cerevisiae significantly inhibited lung metastasis in a dose-dependent manner. Furthermore, therapeutic administration of IS-2 also significantly inhibited the colon 26-M3.1 cell growth in mice. In an assay of liver and spleen metastasis produced by i.v. inoculation of L5178Y-ML25 lymphoma cells, IS-2 also significantly inhibited metastasis in CDF1 mice. Furthermore, pretreatment with IS-2 two days before tumor inoculation significantly prolonged the survival time of tumor-bearing mice. In an in vitro cytotoxicity analysis, IS-2 (up to 100 microg/ml) did not affect the growth of colon 26-M3.1 cells. In contrast, IS-2 enhanced splenocyte proliferating activity in a dose-dependent manner. Peritoneal macrophages stimulated with IS-2 produced various cytokines, such as IL-1beta, IFN-gamma, and IL-12. In addition, treatment with IS-2 (20 microg/mouse) induced tumoricidal activity of peritoneal macrophages against colon 26-M3.1 cells. In an assay for natural killer (NK) cell activity, IS-2 (20 microg/mouse, i.v.) significantly augmented NK cytotoxicity against Yac-1 tumor cells at 2 days after IS-2 treatment. The depletion of NK cells by injection of rabbit anti-asialo GM1 serum abolished the inhibitory effect of IS-2 on lung metastasis of colon 26-M3.1 cells. These data suggest that IS-2 inhibits tumor metastasis via activation of macrophages and NK cells. SN - 1567-5769 UR - https://www.unboundmedicine.com/medline/citation/18068098/Anti_tumor_metastatic_activity_of_beta_glucan_purified_from_mutated_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -