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Identification of tet(M) in two Lactococcus lactis strains isolated from a Spanish traditional starter-free cheese made of raw milk and conjugative transfer of tetracycline resistance to lactococci and enterococci.
Int J Food Microbiol 2008; 121(2):189-94IJ

Abstract

Specific PCR and sequencing showed that a tet(M) gene was present in two tetracycline-resistant Lactococcus lactis strains isolated from a raw milk, starter-free cheese. Hybridisation experiments using as a probe an internal segment of the gene obtained by PCR associated tet(M) with plasmids of around the same size (30 kbp) in both strains. Molecular analysis of the tetracycline resistance loci, including the upstream and downstream regions of the genes, showed them to be identical to one other and to the tet(M) encoded by the conjugative transposon Tn916. Amplification of Tn916-derived segments suggested the transposon was complete in the two L. lactis strains. Further, curing of the tetracycline resistance was accompanied by a reduction in size of the plasmids comparable to that expected for Tn916. Tetracycline resistance could be transferred by conjugation to plasmid-free Lactococcus and Enterococcus strains. However, no plasmid DNA was detected among the transconjugants while both tet(M) and transposon-related sequences were amplified by PCR. This suggested that only the transposon was mobilized.

Authors+Show Affiliations

Instituto de Productos Lácteos de Asturias (CSIC), Carretera de Infiesto s/n, 33300-Villaviciosa, Asturias, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18068255

Citation

Flórez, Ana Belén, et al. "Identification of tet(M) in Two Lactococcus Lactis Strains Isolated From a Spanish Traditional Starter-free Cheese Made of Raw Milk and Conjugative Transfer of Tetracycline Resistance to Lactococci and Enterococci." International Journal of Food Microbiology, vol. 121, no. 2, 2008, pp. 189-94.
Flórez AB, Ammor MS, Mayo B. Identification of tet(M) in two Lactococcus lactis strains isolated from a Spanish traditional starter-free cheese made of raw milk and conjugative transfer of tetracycline resistance to lactococci and enterococci. Int J Food Microbiol. 2008;121(2):189-94.
Flórez, A. B., Ammor, M. S., & Mayo, B. (2008). Identification of tet(M) in two Lactococcus lactis strains isolated from a Spanish traditional starter-free cheese made of raw milk and conjugative transfer of tetracycline resistance to lactococci and enterococci. International Journal of Food Microbiology, 121(2), pp. 189-94.
Flórez AB, Ammor MS, Mayo B. Identification of tet(M) in Two Lactococcus Lactis Strains Isolated From a Spanish Traditional Starter-free Cheese Made of Raw Milk and Conjugative Transfer of Tetracycline Resistance to Lactococci and Enterococci. Int J Food Microbiol. 2008 Jan 31;121(2):189-94. PubMed PMID: 18068255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of tet(M) in two Lactococcus lactis strains isolated from a Spanish traditional starter-free cheese made of raw milk and conjugative transfer of tetracycline resistance to lactococci and enterococci. AU - Flórez,Ana Belén, AU - Ammor,Mohammed Salim, AU - Mayo,Baltasar, Y1 - 2007/11/17/ PY - 2007/02/08/received PY - 2007/08/27/revised PY - 2007/11/06/accepted PY - 2007/12/11/pubmed PY - 2008/4/9/medline PY - 2007/12/11/entrez SP - 189 EP - 94 JF - International journal of food microbiology JO - Int. J. Food Microbiol. VL - 121 IS - 2 N2 - Specific PCR and sequencing showed that a tet(M) gene was present in two tetracycline-resistant Lactococcus lactis strains isolated from a raw milk, starter-free cheese. Hybridisation experiments using as a probe an internal segment of the gene obtained by PCR associated tet(M) with plasmids of around the same size (30 kbp) in both strains. Molecular analysis of the tetracycline resistance loci, including the upstream and downstream regions of the genes, showed them to be identical to one other and to the tet(M) encoded by the conjugative transposon Tn916. Amplification of Tn916-derived segments suggested the transposon was complete in the two L. lactis strains. Further, curing of the tetracycline resistance was accompanied by a reduction in size of the plasmids comparable to that expected for Tn916. Tetracycline resistance could be transferred by conjugation to plasmid-free Lactococcus and Enterococcus strains. However, no plasmid DNA was detected among the transconjugants while both tet(M) and transposon-related sequences were amplified by PCR. This suggested that only the transposon was mobilized. SN - 0168-1605 UR - https://www.unboundmedicine.com/medline/citation/18068255/Identification_of_tet_M__in_two_Lactococcus_lactis_strains_isolated_from_a_Spanish_traditional_starter_free_cheese_made_of_raw_milk_and_conjugative_transfer_of_tetracycline_resistance_to_lactococci_and_enterococci_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1605(07)00602-2 DB - PRIME DP - Unbound Medicine ER -