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Identification of abundant proteins and potential allergens in Culicoides nubeculosus salivary glands.
Vet Immunol Immunopathol. 2008 Mar 15; 122(1-2):94-103.VI

Abstract

IgE-mediated type 1 hypersensitivity reactions to the bites of insects are a common cause of skin disease in horses. Insect bite hypersensitivity (IBH) is most frequently associated with bites of Culicoides spp. and occurs in all parts of the world where horses and Culicoides coexist. The main allergens that cause IBH are probably some of the abundant proteins in the saliva of Culicoides associated with blood feeding. Western blots of Culicoides proteins separated by 1D gel-electrophoresis detected strong IgE responses in all horses with IBH to antigens in protein extracts from wild caught Culicoides, but only weak responses to salivary antigens from captive bred C. nubeculosus which may reflect important differences among allergens from different species of Culicoides or differences between thorax and salivary gland antigens. 2D electrophoresis and mass spectrometry were used to identify several of the abundant proteins in the saliva of C. nubeculosus. These included maltase, members of the D7 family, and several small, basic proteins associated with blood feeding. The most frequently detected IgE-binding proteins were in a group of proteins with pI>8.5 and mass 40-50kDa. Mass spectrometry identified two of these allergenic proteins as similar to hyaluronidase and a heavily glycosylated protein of unknown function that have previously been identified in salivary glands of C. sonorensis.

Authors+Show Affiliations

University of Bristol, School of Clinical Veterinary Science, Langford House, Langford, Bristol BS40 5DU, UK. doug.wilson@bris.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18068808

Citation

Wilson, A D., et al. "Identification of Abundant Proteins and Potential Allergens in Culicoides Nubeculosus Salivary Glands." Veterinary Immunology and Immunopathology, vol. 122, no. 1-2, 2008, pp. 94-103.
Wilson AD, Heesom KJ, Mawby WJ, et al. Identification of abundant proteins and potential allergens in Culicoides nubeculosus salivary glands. Vet Immunol Immunopathol. 2008;122(1-2):94-103.
Wilson, A. D., Heesom, K. J., Mawby, W. J., Mellor, P. S., & Russell, C. L. (2008). Identification of abundant proteins and potential allergens in Culicoides nubeculosus salivary glands. Veterinary Immunology and Immunopathology, 122(1-2), 94-103.
Wilson AD, et al. Identification of Abundant Proteins and Potential Allergens in Culicoides Nubeculosus Salivary Glands. Vet Immunol Immunopathol. 2008 Mar 15;122(1-2):94-103. PubMed PMID: 18068808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of abundant proteins and potential allergens in Culicoides nubeculosus salivary glands. AU - Wilson,A D, AU - Heesom,K J, AU - Mawby,W J, AU - Mellor,P S, AU - Russell,C L, Y1 - 2007/10/25/ PY - 2007/08/09/received PY - 2007/10/11/revised PY - 2007/10/18/accepted PY - 2007/12/11/pubmed PY - 2008/4/23/medline PY - 2007/12/11/entrez SP - 94 EP - 103 JF - Veterinary immunology and immunopathology JO - Vet Immunol Immunopathol VL - 122 IS - 1-2 N2 - IgE-mediated type 1 hypersensitivity reactions to the bites of insects are a common cause of skin disease in horses. Insect bite hypersensitivity (IBH) is most frequently associated with bites of Culicoides spp. and occurs in all parts of the world where horses and Culicoides coexist. The main allergens that cause IBH are probably some of the abundant proteins in the saliva of Culicoides associated with blood feeding. Western blots of Culicoides proteins separated by 1D gel-electrophoresis detected strong IgE responses in all horses with IBH to antigens in protein extracts from wild caught Culicoides, but only weak responses to salivary antigens from captive bred C. nubeculosus which may reflect important differences among allergens from different species of Culicoides or differences between thorax and salivary gland antigens. 2D electrophoresis and mass spectrometry were used to identify several of the abundant proteins in the saliva of C. nubeculosus. These included maltase, members of the D7 family, and several small, basic proteins associated with blood feeding. The most frequently detected IgE-binding proteins were in a group of proteins with pI>8.5 and mass 40-50kDa. Mass spectrometry identified two of these allergenic proteins as similar to hyaluronidase and a heavily glycosylated protein of unknown function that have previously been identified in salivary glands of C. sonorensis. SN - 0165-2427 UR - https://www.unboundmedicine.com/medline/citation/18068808/Identification_of_abundant_proteins_and_potential_allergens_in_Culicoides_nubeculosus_salivary_glands_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2427(07)00384-4 DB - PRIME DP - Unbound Medicine ER -