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PCR detection of Staphylococcal enterotoxins (SEs) N, O, P, Q, R, U, and survey of SE types in Staphylococcus aureus isolates from food-poisoning cases in Taiwan.
Int J Food Microbiol. 2008 Jan 15; 121(1):66-73.IJ

Abstract

Staphylococcal enterotoxins (SEs) are superantigenic toxins. They are five major classical types, i.e., SEA, SEB, SEC, SED, SEE, and new SEs or SE-like superantigens, such as SEG to SEU. Only the staphylococcal superantigens (SAgs) that induce emesis following oral administration in a monkey model are designated as SEs while other related toxins are called SE-like (SEl) superantigens. To survey the enterotoxin genotypes for S. aureus strains isolated from food-poisoning cases in Taiwan, we developed PCR primers specific for SEN, SEO, SEP, SEQ, SER, and SEU genes. The complete SE sequences and their expression potential for strains positive to sen, seo, sep, seq, ser, and seu specific primers were also determined. These strains were used as reference strains. With the PCR primers specific for all SEs or SAgs, including toxic shock syndrome toxin I (TSST-1), we assayed the genotypes of 147 S. aureus strains isolated from patients associated with staphylococcal food-poisoning outbreaks occurred during 2001-2003. For these 147 strains, 135 (91.8%) were found positive for one or more SE or SAg genes. For classical enterotoxin and TSST-1 types, the major one was tsst-1 (59.1%) following by sea (29.2%), seb (19.7%), sec (6.8%), and sed (2.0%). For new SE and SAg types, the major one was sei (29.9%) and sep (27.9%) followed by, sek (16.3%), seo (14.3%), seu (14.2%), sem (11.6%), sen (10.9%), seq (10.9%), seh (8.2%), sel (6.8%), and ser (5.4%) etc. This report reveals the whole SE and SAg genotypes for S. aureus strains isolated from staphylococcal food-poisoning cases in Taiwan.

Authors+Show Affiliations

Institute of Industrial Biotechnology, Hung Kuang University, 34 Chung-Chi Road, Shalu, Taichung County, 433, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18068843

Citation

Chiang, Yu-Cheng, et al. "PCR Detection of Staphylococcal Enterotoxins (SEs) N, O, P, Q, R, U, and Survey of SE Types in Staphylococcus Aureus Isolates From Food-poisoning Cases in Taiwan." International Journal of Food Microbiology, vol. 121, no. 1, 2008, pp. 66-73.
Chiang YC, Liao WW, Fan CM, et al. PCR detection of Staphylococcal enterotoxins (SEs) N, O, P, Q, R, U, and survey of SE types in Staphylococcus aureus isolates from food-poisoning cases in Taiwan. Int J Food Microbiol. 2008;121(1):66-73.
Chiang, Y. C., Liao, W. W., Fan, C. M., Pai, W. Y., Chiou, C. S., & Tsen, H. Y. (2008). PCR detection of Staphylococcal enterotoxins (SEs) N, O, P, Q, R, U, and survey of SE types in Staphylococcus aureus isolates from food-poisoning cases in Taiwan. International Journal of Food Microbiology, 121(1), 66-73.
Chiang YC, et al. PCR Detection of Staphylococcal Enterotoxins (SEs) N, O, P, Q, R, U, and Survey of SE Types in Staphylococcus Aureus Isolates From Food-poisoning Cases in Taiwan. Int J Food Microbiol. 2008 Jan 15;121(1):66-73. PubMed PMID: 18068843.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PCR detection of Staphylococcal enterotoxins (SEs) N, O, P, Q, R, U, and survey of SE types in Staphylococcus aureus isolates from food-poisoning cases in Taiwan. AU - Chiang,Yu-Cheng, AU - Liao,Wan-Wen, AU - Fan,Chin-Ming, AU - Pai,Wan-Yu, AU - Chiou,Chien-Shun, AU - Tsen,Hau-Yang, Y1 - 2007/11/01/ PY - 2007/03/08/received PY - 2007/09/15/revised PY - 2007/10/24/accepted PY - 2007/12/11/pubmed PY - 2008/4/11/medline PY - 2007/12/11/entrez SP - 66 EP - 73 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 121 IS - 1 N2 - Staphylococcal enterotoxins (SEs) are superantigenic toxins. They are five major classical types, i.e., SEA, SEB, SEC, SED, SEE, and new SEs or SE-like superantigens, such as SEG to SEU. Only the staphylococcal superantigens (SAgs) that induce emesis following oral administration in a monkey model are designated as SEs while other related toxins are called SE-like (SEl) superantigens. To survey the enterotoxin genotypes for S. aureus strains isolated from food-poisoning cases in Taiwan, we developed PCR primers specific for SEN, SEO, SEP, SEQ, SER, and SEU genes. The complete SE sequences and their expression potential for strains positive to sen, seo, sep, seq, ser, and seu specific primers were also determined. These strains were used as reference strains. With the PCR primers specific for all SEs or SAgs, including toxic shock syndrome toxin I (TSST-1), we assayed the genotypes of 147 S. aureus strains isolated from patients associated with staphylococcal food-poisoning outbreaks occurred during 2001-2003. For these 147 strains, 135 (91.8%) were found positive for one or more SE or SAg genes. For classical enterotoxin and TSST-1 types, the major one was tsst-1 (59.1%) following by sea (29.2%), seb (19.7%), sec (6.8%), and sed (2.0%). For new SE and SAg types, the major one was sei (29.9%) and sep (27.9%) followed by, sek (16.3%), seo (14.3%), seu (14.2%), sem (11.6%), sen (10.9%), seq (10.9%), seh (8.2%), sel (6.8%), and ser (5.4%) etc. This report reveals the whole SE and SAg genotypes for S. aureus strains isolated from staphylococcal food-poisoning cases in Taiwan. SN - 0168-1605 UR - https://www.unboundmedicine.com/medline/citation/18068843/PCR_detection_of_Staphylococcal_enterotoxins__SEs__N_O_P_Q_R_U_and_survey_of_SE_types_in_Staphylococcus_aureus_isolates_from_food_poisoning_cases_in_Taiwan_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1605(07)00559-4 DB - PRIME DP - Unbound Medicine ER -