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Chitotriosidase analysis in bronchoalveolar lavage of patients with sarcoidosis.
Sarcoidosis Vasc Diffuse Lung Dis. 2007 Mar; 24(1):59-64.SV

Abstract

BACKGROUND

Sarcoidosis is a systemic granulomatous disease characterised by T-helper cell/macrophage alveolitis. Activated macrophages release mediators, such as cytokines, chemokines, oxygen radicals, and enzymes. In a previous paper we found higher levels of chitotriosidase, a macrophage derived enzyme, in serum of patients with sarcoidosis than in controls. Serum chitotriosidase levels were correlated with sarcoidosis radiological stages. Human chitotriosidase is involved in the pathogenesis of many lysosomal storage disorders and is selectively expressed in chronically activated tissue macrophages.

METHODS

In the present study we determined chitotriosidase concentrations in bronchoalveolar lavage of patients with newly diagnosed pulmonary sarcoidosis (divided into two groups according to clinical parameters) and of controls with an ELISA test.

RESULTS

Significantly different chitotriosidase concentrations were found in BAL of patients than controls, especially in patients with progressing disease.

CONCLUSION

Chitotriosidase but not angiotensin converting enzyme concentrations correlated with sarcoidosis radiological stages, and also with the degree of lung infiltrate seen by CT-scan, suggesting that the former enzyme (detected locally and sistemically) may play a role in the pathogenesis of the disease. Further studies with a greater number of patients are needed to confirm this hypothesis and to determine whether chitotriosidase may be a marker of the severity of sarcoidosis.

Authors+Show Affiliations

Respiratory Diseases Section, Department of Clinical Medicine and Immunology, Le Scotte Hospital, Viale Bracci 53100-Siena, Italy. bargagli2@unisi.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18069420

Citation

Bargagli, Elena, et al. "Chitotriosidase Analysis in Bronchoalveolar Lavage of Patients With Sarcoidosis." Sarcoidosis, Vasculitis, and Diffuse Lung Diseases : Official Journal of WASOG, vol. 24, no. 1, 2007, pp. 59-64.
Bargagli E, Margollicci M, Perrone A, et al. Chitotriosidase analysis in bronchoalveolar lavage of patients with sarcoidosis. Sarcoidosis Vasc Diffuse Lung Dis. 2007;24(1):59-64.
Bargagli, E., Margollicci, M., Perrone, A., Luddi, A., Perari, M. G., Bianchi, N., Refini, R. M., Grosso, S., Volterrani, L., & Rottoli, P. (2007). Chitotriosidase analysis in bronchoalveolar lavage of patients with sarcoidosis. Sarcoidosis, Vasculitis, and Diffuse Lung Diseases : Official Journal of WASOG, 24(1), 59-64.
Bargagli E, et al. Chitotriosidase Analysis in Bronchoalveolar Lavage of Patients With Sarcoidosis. Sarcoidosis Vasc Diffuse Lung Dis. 2007;24(1):59-64. PubMed PMID: 18069420.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chitotriosidase analysis in bronchoalveolar lavage of patients with sarcoidosis. AU - Bargagli,Elena, AU - Margollicci,Maria, AU - Perrone,Anna, AU - Luddi,Alice, AU - Perari,Maria Grazia, AU - Bianchi,Nicola, AU - Refini,Rosa Metella, AU - Grosso,Salvatore, AU - Volterrani,Luca, AU - Rottoli,Paola, PY - 2007/12/12/pubmed PY - 2008/2/12/medline PY - 2007/12/12/entrez SP - 59 EP - 64 JF - Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG JO - Sarcoidosis Vasc Diffuse Lung Dis VL - 24 IS - 1 N2 - BACKGROUND: Sarcoidosis is a systemic granulomatous disease characterised by T-helper cell/macrophage alveolitis. Activated macrophages release mediators, such as cytokines, chemokines, oxygen radicals, and enzymes. In a previous paper we found higher levels of chitotriosidase, a macrophage derived enzyme, in serum of patients with sarcoidosis than in controls. Serum chitotriosidase levels were correlated with sarcoidosis radiological stages. Human chitotriosidase is involved in the pathogenesis of many lysosomal storage disorders and is selectively expressed in chronically activated tissue macrophages. METHODS: In the present study we determined chitotriosidase concentrations in bronchoalveolar lavage of patients with newly diagnosed pulmonary sarcoidosis (divided into two groups according to clinical parameters) and of controls with an ELISA test. RESULTS: Significantly different chitotriosidase concentrations were found in BAL of patients than controls, especially in patients with progressing disease. CONCLUSION: Chitotriosidase but not angiotensin converting enzyme concentrations correlated with sarcoidosis radiological stages, and also with the degree of lung infiltrate seen by CT-scan, suggesting that the former enzyme (detected locally and sistemically) may play a role in the pathogenesis of the disease. Further studies with a greater number of patients are needed to confirm this hypothesis and to determine whether chitotriosidase may be a marker of the severity of sarcoidosis. SN - 1124-0490 UR - https://www.unboundmedicine.com/medline/citation/18069420/Chitotriosidase_analysis_in_bronchoalveolar_lavage_of_patients_with_sarcoidosis_ L2 - http://www.diseaseinfosearch.org/result/6412 DB - PRIME DP - Unbound Medicine ER -