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Mapping quantitative trait loci associated with barley net blotch resistance.
Theor Appl Genet. 2008 Feb; 116(4):529-39.TA

Abstract

Net blotch of barley, caused by Pyrenophora teres Drechs., is an important foliar disease worldwide. Deployment of resistant cultivars is the most economic and eco-friendly control method. This report describes mapping of quantitative trait loci (QTL) associated with net blotch resistance in a doubled-haploid (DH) barley population using diversity arrays technology (DArT) markers. One hundred and fifty DH lines from the cross CDC Dolly (susceptible)/TR251 (resistant) were screened as seedlings in controlled environments with net-form net blotch (NFNB) isolates WRS858 and WRS1607 and spot-form net blotch (SFNB) isolate WRS857. The population was also screened at the adult-plant stage for NFNB resistance in the field in 2005 and 2006. A high-density genetic linkage map of 90 DH lines was constructed using 457 DArT and 11 SSR markers. A major NFNB seedling resistance QTL, designated QRpt6, was mapped to chromosome 6H for isolates WRS858 and WRS1607. QRpt6 was associated with adult-plant resistance in the 2005 and 2006 field trials. Additional QTL for NFNB seedling resistance to the more virulent isolate WRS858 were identified on chromosomes 2H, 4H, and 5H. A seedling resistance QTL (QRpts4) for the SFNB isolate WRS857 was detected on chromosome 4H as was a significant QTL (QRpt7) on chromosome 7H. Three QTL (QRpt6, QRpts4, QRpt7) were associated with resistance to both net blotch forms and lines with one or more of these demonstrated improved resistance. Simple sequence repeat (SSR) markers tightly linked to QRpt6 and QRpts4 were identified and validated in an unrelated barley population. The major 6H QTL, QRpt6, may provide adequate NFNB field resistance in western Canada and could be routinely selected for using molecular markers in a practical breeding program.

Authors+Show Affiliations

Department of Plant Sciences, Crop Development Centre, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada. tajinder.grewal@usask.caNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18071668

Citation

Grewal, T S., et al. "Mapping Quantitative Trait Loci Associated With Barley Net Blotch Resistance." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 116, no. 4, 2008, pp. 529-39.
Grewal TS, Rossnagel BG, Pozniak CJ, et al. Mapping quantitative trait loci associated with barley net blotch resistance. Theor Appl Genet. 2008;116(4):529-39.
Grewal, T. S., Rossnagel, B. G., Pozniak, C. J., & Scoles, G. J. (2008). Mapping quantitative trait loci associated with barley net blotch resistance. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 116(4), 529-39.
Grewal TS, et al. Mapping Quantitative Trait Loci Associated With Barley Net Blotch Resistance. Theor Appl Genet. 2008;116(4):529-39. PubMed PMID: 18071668.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mapping quantitative trait loci associated with barley net blotch resistance. AU - Grewal,T S, AU - Rossnagel,B G, AU - Pozniak,C J, AU - Scoles,G J, Y1 - 2007/12/11/ PY - 2007/03/12/received PY - 2007/11/28/accepted PY - 2007/12/12/pubmed PY - 2008/4/30/medline PY - 2007/12/12/entrez SP - 529 EP - 39 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 116 IS - 4 N2 - Net blotch of barley, caused by Pyrenophora teres Drechs., is an important foliar disease worldwide. Deployment of resistant cultivars is the most economic and eco-friendly control method. This report describes mapping of quantitative trait loci (QTL) associated with net blotch resistance in a doubled-haploid (DH) barley population using diversity arrays technology (DArT) markers. One hundred and fifty DH lines from the cross CDC Dolly (susceptible)/TR251 (resistant) were screened as seedlings in controlled environments with net-form net blotch (NFNB) isolates WRS858 and WRS1607 and spot-form net blotch (SFNB) isolate WRS857. The population was also screened at the adult-plant stage for NFNB resistance in the field in 2005 and 2006. A high-density genetic linkage map of 90 DH lines was constructed using 457 DArT and 11 SSR markers. A major NFNB seedling resistance QTL, designated QRpt6, was mapped to chromosome 6H for isolates WRS858 and WRS1607. QRpt6 was associated with adult-plant resistance in the 2005 and 2006 field trials. Additional QTL for NFNB seedling resistance to the more virulent isolate WRS858 were identified on chromosomes 2H, 4H, and 5H. A seedling resistance QTL (QRpts4) for the SFNB isolate WRS857 was detected on chromosome 4H as was a significant QTL (QRpt7) on chromosome 7H. Three QTL (QRpt6, QRpts4, QRpt7) were associated with resistance to both net blotch forms and lines with one or more of these demonstrated improved resistance. Simple sequence repeat (SSR) markers tightly linked to QRpt6 and QRpts4 were identified and validated in an unrelated barley population. The major 6H QTL, QRpt6, may provide adequate NFNB field resistance in western Canada and could be routinely selected for using molecular markers in a practical breeding program. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/18071668/Mapping_quantitative_trait_loci_associated_with_barley_net_blotch_resistance_ L2 - https://dx.doi.org/10.1007/s00122-007-0688-9 DB - PRIME DP - Unbound Medicine ER -