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Integration of human papillomavirus type-16 and type-18 is a very early event in cervical carcinogenesis.
J Clin Pathol. 2008 May; 61(5):627-31.JC

Abstract

AIM

Human papillomavirus (HPV) integration is a critical event in cervical carcinogenesis. The aim of this study was to explore the physical status of HPV-16 and HPV-18 during the progression of cervical precancerous lesions.

METHODS

A series of 101 HPV-16 or HPV-18 positive cervical neoplasms (32 cervical intraepithelial neoplasia (CIN) cases and 69 cervical carcinoma (CC) cases) were evaluated. The physical status of both types of HPV was assessed from paraffin-embedded formaldehyde-fixed surgical specimens by real-time PCR.

RESULTS

For HPV-16, integrated DNA was observed in 5 (83.3%) of 6 CIN I cases, 10 (90.9%) of 11 CIN II/III cases, 29 (82.9%) of 35 FIGO (International Federation of Gynecology and Obstetrics) stage I CC cases and 16 (94.1%) of 17 FIGO stages II approximately IV CC cases. For HPV-18, integrated DNA was observed in 3 (50%) of 6 CIN I cases, 5 (55.6%) of 9 CIN II/III cases, 9 (64.3%) of 14 FIGO stage I CC cases, and 1 (33.3%) of 3 FIGO stages II approximately IV CC cases. The mixed form of HPV DNA was the most prevalent physical state in HPV-16. There was no significant difference between the physical state of HPV-16 and HPV-18 DNA with regard to the various grades of cervical lesions.

CONCLUSIONS

These data imply that integration of HPV-16 and HPV-18 DNA into the host genome occurs in the very early stage of cervical neoplastic progression. These early events may play an initiating role in the malignant transformation of HPV-16- and HPV-18-related low-grade lesions into high-grade dysplasia and invasive carcinoma.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan. m002057@ms.skh.org.twNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18077768

Citation

Huang, L-W, et al. "Integration of Human Papillomavirus Type-16 and Type-18 Is a Very Early Event in Cervical Carcinogenesis." Journal of Clinical Pathology, vol. 61, no. 5, 2008, pp. 627-31.
Huang LW, Chao SL, Lee BH. Integration of human papillomavirus type-16 and type-18 is a very early event in cervical carcinogenesis. J Clin Pathol. 2008;61(5):627-31.
Huang, L. W., Chao, S. L., & Lee, B. H. (2008). Integration of human papillomavirus type-16 and type-18 is a very early event in cervical carcinogenesis. Journal of Clinical Pathology, 61(5), 627-31.
Huang LW, Chao SL, Lee BH. Integration of Human Papillomavirus Type-16 and Type-18 Is a Very Early Event in Cervical Carcinogenesis. J Clin Pathol. 2008;61(5):627-31. PubMed PMID: 18077768.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Integration of human papillomavirus type-16 and type-18 is a very early event in cervical carcinogenesis. AU - Huang,L-W, AU - Chao,S-L, AU - Lee,B-H, Y1 - 2007/12/12/ PY - 2007/12/14/pubmed PY - 2008/5/30/medline PY - 2007/12/14/entrez SP - 627 EP - 31 JF - Journal of clinical pathology JO - J. Clin. Pathol. VL - 61 IS - 5 N2 - AIM: Human papillomavirus (HPV) integration is a critical event in cervical carcinogenesis. The aim of this study was to explore the physical status of HPV-16 and HPV-18 during the progression of cervical precancerous lesions. METHODS: A series of 101 HPV-16 or HPV-18 positive cervical neoplasms (32 cervical intraepithelial neoplasia (CIN) cases and 69 cervical carcinoma (CC) cases) were evaluated. The physical status of both types of HPV was assessed from paraffin-embedded formaldehyde-fixed surgical specimens by real-time PCR. RESULTS: For HPV-16, integrated DNA was observed in 5 (83.3%) of 6 CIN I cases, 10 (90.9%) of 11 CIN II/III cases, 29 (82.9%) of 35 FIGO (International Federation of Gynecology and Obstetrics) stage I CC cases and 16 (94.1%) of 17 FIGO stages II approximately IV CC cases. For HPV-18, integrated DNA was observed in 3 (50%) of 6 CIN I cases, 5 (55.6%) of 9 CIN II/III cases, 9 (64.3%) of 14 FIGO stage I CC cases, and 1 (33.3%) of 3 FIGO stages II approximately IV CC cases. The mixed form of HPV DNA was the most prevalent physical state in HPV-16. There was no significant difference between the physical state of HPV-16 and HPV-18 DNA with regard to the various grades of cervical lesions. CONCLUSIONS: These data imply that integration of HPV-16 and HPV-18 DNA into the host genome occurs in the very early stage of cervical neoplastic progression. These early events may play an initiating role in the malignant transformation of HPV-16- and HPV-18-related low-grade lesions into high-grade dysplasia and invasive carcinoma. SN - 1472-4146 UR - https://www.unboundmedicine.com/medline/citation/18077768/Integration_of_human_papillomavirus_type_16_and_type_18_is_a_very_early_event_in_cervical_carcinogenesis_ L2 - http://jcp.bmj.com/cgi/pmidlookup?view=long&pmid=18077768 DB - PRIME DP - Unbound Medicine ER -