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Evaluation of in vivo efficacy of topical formulations containing soybean extract.
Int J Pharm. 2008 Mar 20; 352(1-2):189-96.IJ

Abstract

In the present study it was evaluated the: (i) functional stability of the soybean extract as a raw material and dispersed in two different topical formulations, (ii) skin retention using modified Franz diffusion cells, and (iii) in vivo activity of these formulations to inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced hydrogen peroxide (H(2)O(2)) and malondialdehyde (MDA) increases in the skin of hairless mice. The physico-chemical stability was evaluated by pH, globule size and centrifugation test. Furthermore, functional stability was also evaluated by antilipoperoxidative activity. The two topical formulations were stored at 4 degrees C, 30 degrees C/60% RH and 40 degrees C/70% RH for 6 months. The evaluation of the antiperoxidative stability of soybean extract itself and incorporated in formulations did not demonstrate loss of activity by storage at 4 degrees C/6 months. During 6 months of the study in different storage conditions the formulations 1 and 2 added or not with soybean extract were stable to physico-chemical tests. The effect of antioxidant compounds detected by the inhibition of MDA formation was time-dependent for formulation 2 as detected in the skin retention study. Pretreatment with formulation 1 or 2 significantly diminished TPA-induced H(2)O(2) and MDA generation. In conclusion, the present results suggest for the first time that formulations containing soybean extract may be a topical source of antioxidant compounds that decrease oxidative damages of the skin.

Authors+Show Affiliations

Department of Pharmaceutical Science, Faculty of Pharmaceutical Sciences of Ribeirao Preto - USP, Avenue Do Café s/n, CEP 14040-903, Ribeirao Preto, SP, Brazil. sangeorgetti@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18079076

Citation

Georgetti, Sandra R., et al. "Evaluation of in Vivo Efficacy of Topical Formulations Containing Soybean Extract." International Journal of Pharmaceutics, vol. 352, no. 1-2, 2008, pp. 189-96.
Georgetti SR, Casagrande R, Verri WA, et al. Evaluation of in vivo efficacy of topical formulations containing soybean extract. Int J Pharm. 2008;352(1-2):189-96.
Georgetti, S. R., Casagrande, R., Verri, W. A., Lopez, R. F., & Fonseca, M. J. (2008). Evaluation of in vivo efficacy of topical formulations containing soybean extract. International Journal of Pharmaceutics, 352(1-2), 189-96.
Georgetti SR, et al. Evaluation of in Vivo Efficacy of Topical Formulations Containing Soybean Extract. Int J Pharm. 2008 Mar 20;352(1-2):189-96. PubMed PMID: 18079076.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of in vivo efficacy of topical formulations containing soybean extract. AU - Georgetti,Sandra R, AU - Casagrande,Rúbia, AU - Verri,Waldiceu A,Jr AU - Lopez,Renata F V, AU - Fonseca,Maria J V, Y1 - 2007/11/04/ PY - 2007/06/08/received PY - 2007/09/24/revised PY - 2007/10/24/accepted PY - 2007/12/15/pubmed PY - 2008/7/23/medline PY - 2007/12/15/entrez SP - 189 EP - 96 JF - International journal of pharmaceutics JO - Int J Pharm VL - 352 IS - 1-2 N2 - In the present study it was evaluated the: (i) functional stability of the soybean extract as a raw material and dispersed in two different topical formulations, (ii) skin retention using modified Franz diffusion cells, and (iii) in vivo activity of these formulations to inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced hydrogen peroxide (H(2)O(2)) and malondialdehyde (MDA) increases in the skin of hairless mice. The physico-chemical stability was evaluated by pH, globule size and centrifugation test. Furthermore, functional stability was also evaluated by antilipoperoxidative activity. The two topical formulations were stored at 4 degrees C, 30 degrees C/60% RH and 40 degrees C/70% RH for 6 months. The evaluation of the antiperoxidative stability of soybean extract itself and incorporated in formulations did not demonstrate loss of activity by storage at 4 degrees C/6 months. During 6 months of the study in different storage conditions the formulations 1 and 2 added or not with soybean extract were stable to physico-chemical tests. The effect of antioxidant compounds detected by the inhibition of MDA formation was time-dependent for formulation 2 as detected in the skin retention study. Pretreatment with formulation 1 or 2 significantly diminished TPA-induced H(2)O(2) and MDA generation. In conclusion, the present results suggest for the first time that formulations containing soybean extract may be a topical source of antioxidant compounds that decrease oxidative damages of the skin. SN - 0378-5173 UR - https://www.unboundmedicine.com/medline/citation/18079076/Evaluation_of_in_vivo_efficacy_of_topical_formulations_containing_soybean_extract_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-5173(07)00871-X DB - PRIME DP - Unbound Medicine ER -