Tags

Type your tag names separated by a space and hit enter

Lycopus lucidus inhibits high glucose-induced vascular inflammation in human umbilical vein endothelial cells.
Vascul Pharmacol. 2008 Jan; 48(1):38-46.VP

Abstract

Vascular inflammatory process has been suggested to play a key role in the initiation and progression of atherosclerosis, a major complication of diabetes mellitus. Lycopus lucidus Turcz. has been used as an oriental traditional medicine including Korea and its crude drug is known to have an anti-inflammatory effect. Thus we investigated whether the aqueous extract of the leaves of L. lucidus Turcz. (ALT) suppresses vascular inflammatory process induced by high glucose in primary cultured human umbilical vein endothelial cells (HUVEC). Western blot analysis revealed that incubation of HUVEC with high glucose increased cell adhesion molecules (CAMs) expression levels. However, high glucose-induced increase of CAMs expression was significantly attenuated by pretreatment with ALT in a dose-dependent manner. The enhanced cell adhesion between monocyte and HUVEC induced by high glucose was also blocked by pretreatment with ALT. High glucose-induced hydrogen peroxide production and DCF-sensitive intracellular reactive oxygen species (ROS) formation. Pretreatment with ALT inhibited high glucose-induced ROS formation. In addition, ALT suppressed the translocation and promoter transcriptional activity of NF-kappaB increased in high glucose condition. Taken together, the present data suggested that ALT could suppress high glucose-induced vascular inflammatory process, which may be closely related with the inhibition of ROS and NF-kappaB activation in HUVEC.

Authors+Show Affiliations

Professional Graduate School of Oriental Medicine, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18083068

Citation

Lee, Yun Jung, et al. "Lycopus Lucidus Inhibits High Glucose-induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells." Vascular Pharmacology, vol. 48, no. 1, 2008, pp. 38-46.
Lee YJ, Kang DG, Kim JS, et al. Lycopus lucidus inhibits high glucose-induced vascular inflammation in human umbilical vein endothelial cells. Vascul Pharmacol. 2008;48(1):38-46.
Lee, Y. J., Kang, D. G., Kim, J. S., & Lee, H. S. (2008). Lycopus lucidus inhibits high glucose-induced vascular inflammation in human umbilical vein endothelial cells. Vascular Pharmacology, 48(1), 38-46.
Lee YJ, et al. Lycopus Lucidus Inhibits High Glucose-induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells. Vascul Pharmacol. 2008;48(1):38-46. PubMed PMID: 18083068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lycopus lucidus inhibits high glucose-induced vascular inflammation in human umbilical vein endothelial cells. AU - Lee,Yun Jung, AU - Kang,Dae Gill, AU - Kim,Jin Sook, AU - Lee,Ho Sub, Y1 - 2007/11/21/ PY - 2007/05/25/received PY - 2007/10/19/revised PY - 2007/11/06/accepted PY - 2007/12/18/pubmed PY - 2008/5/13/medline PY - 2007/12/18/entrez SP - 38 EP - 46 JF - Vascular pharmacology JO - Vascul Pharmacol VL - 48 IS - 1 N2 - Vascular inflammatory process has been suggested to play a key role in the initiation and progression of atherosclerosis, a major complication of diabetes mellitus. Lycopus lucidus Turcz. has been used as an oriental traditional medicine including Korea and its crude drug is known to have an anti-inflammatory effect. Thus we investigated whether the aqueous extract of the leaves of L. lucidus Turcz. (ALT) suppresses vascular inflammatory process induced by high glucose in primary cultured human umbilical vein endothelial cells (HUVEC). Western blot analysis revealed that incubation of HUVEC with high glucose increased cell adhesion molecules (CAMs) expression levels. However, high glucose-induced increase of CAMs expression was significantly attenuated by pretreatment with ALT in a dose-dependent manner. The enhanced cell adhesion between monocyte and HUVEC induced by high glucose was also blocked by pretreatment with ALT. High glucose-induced hydrogen peroxide production and DCF-sensitive intracellular reactive oxygen species (ROS) formation. Pretreatment with ALT inhibited high glucose-induced ROS formation. In addition, ALT suppressed the translocation and promoter transcriptional activity of NF-kappaB increased in high glucose condition. Taken together, the present data suggested that ALT could suppress high glucose-induced vascular inflammatory process, which may be closely related with the inhibition of ROS and NF-kappaB activation in HUVEC. SN - 1537-1891 UR - https://www.unboundmedicine.com/medline/citation/18083068/Lycopus_lucidus_inhibits_high_glucose_induced_vascular_inflammation_in_human_umbilical_vein_endothelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1537-1891(07)00158-9 DB - PRIME DP - Unbound Medicine ER -