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Carbon nanofiber-based composites for the construction of mediator-free biosensors.
Biosens Bioelectron. 2008 Mar 14; 23(8):1236-43.BB

Abstract

Carbon nanofibers (CNFs), with typical diameters of approximately 80 nm and lengths of the order of micrometers, are extremely attractive in bioanalytical area as they can combine properties of high surface area, non-toxicity, acceptable biocompatibility, ease of fabrication, chemical and electrochemical stability, good electrical conductivity. In this work, CNF-based composites were successfully used as an immobilization matrix for the construction of a reagentless mediator-free hemoglobin-based H2O2 biosensor. The results revealed that hemoglobin retained its essential secondary structure in the CNF-based composite film. With the advantages of organic-inorganic hybrid materials, dramatically facilitated direct electron transfer of hemoglobin and good bioelectrocatalytic activity towards H2O2 were demonstrated. The biosensor displayed good performance along with good long-term stability. The CNF-based composites were proved to be a promising biosensing platform for the construction of mediator-free biosensors, and may find wide potential applications in biosensors, biocatalysis, bioelectronics and biofuel cell.

Authors+Show Affiliations

Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18083363

Citation

Lu, Xianbo, et al. "Carbon Nanofiber-based Composites for the Construction of Mediator-free Biosensors." Biosensors & Bioelectronics, vol. 23, no. 8, 2008, pp. 1236-43.
Lu X, Zhou J, Lu W, et al. Carbon nanofiber-based composites for the construction of mediator-free biosensors. Biosens Bioelectron. 2008;23(8):1236-43.
Lu, X., Zhou, J., Lu, W., Liu, Q., & Li, J. (2008). Carbon nanofiber-based composites for the construction of mediator-free biosensors. Biosensors & Bioelectronics, 23(8), 1236-43.
Lu X, et al. Carbon Nanofiber-based Composites for the Construction of Mediator-free Biosensors. Biosens Bioelectron. 2008 Mar 14;23(8):1236-43. PubMed PMID: 18083363.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carbon nanofiber-based composites for the construction of mediator-free biosensors. AU - Lu,Xianbo, AU - Zhou,Jianhua, AU - Lu,Wu, AU - Liu,Qing, AU - Li,Jinghong, Y1 - 2007/11/17/ PY - 2007/09/28/received PY - 2007/11/02/accepted PY - 2007/12/18/pubmed PY - 2008/5/30/medline PY - 2007/12/18/entrez SP - 1236 EP - 43 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 23 IS - 8 N2 - Carbon nanofibers (CNFs), with typical diameters of approximately 80 nm and lengths of the order of micrometers, are extremely attractive in bioanalytical area as they can combine properties of high surface area, non-toxicity, acceptable biocompatibility, ease of fabrication, chemical and electrochemical stability, good electrical conductivity. In this work, CNF-based composites were successfully used as an immobilization matrix for the construction of a reagentless mediator-free hemoglobin-based H2O2 biosensor. The results revealed that hemoglobin retained its essential secondary structure in the CNF-based composite film. With the advantages of organic-inorganic hybrid materials, dramatically facilitated direct electron transfer of hemoglobin and good bioelectrocatalytic activity towards H2O2 were demonstrated. The biosensor displayed good performance along with good long-term stability. The CNF-based composites were proved to be a promising biosensing platform for the construction of mediator-free biosensors, and may find wide potential applications in biosensors, biocatalysis, bioelectronics and biofuel cell. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/18083363/Carbon_nanofiber_based_composites_for_the_construction_of_mediator_free_biosensors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(07)00474-5 DB - PRIME DP - Unbound Medicine ER -