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Anaerobic conditions promote expression of Sfp fimbriae and adherence of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM to human intestinal epithelial cells.
Appl Environ Microbiol. 2008 Feb; 74(4):1087-93.AE

Abstract

The sfp gene cluster, unique to sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:NM strains, encodes fimbriae that mediate mannose-resistant hemagglutination in laboratory E. coli strains but are not expressed in wild-type SF EHEC O157:NM strains under standard laboratory conditions. We investigated whether Sfp fimbriae are expressed under conditions that mimic the intestinal environment and whether they contribute to the adherence of SF EHEC O157:NM strains to human intestinal epithelial cells. The transcription of sfpA (encoding the major fimbrial subunit) was upregulated in all strains investigated, and all expressed SfpA and possessed fimbriae that reacted with an anti-SfpA antibody when the strains were grown on solid media under anaerobic conditions. Sfp expression was absent under aerobic conditions and in liquid media. Sfp upregulation under anaerobic conditions was significantly higher on blood agar and a medium simulating the colonic environment than on a medium simulating the ileal environment (P < 0.05). The induction of Sfp fimbriae in SF E. coli O157:NM strains correlates with increased adherence to Caco-2 and HCT-8 cells. Our data indicate that the expression of Sfp fimbriae in SF E. coli O157:NM strains is induced under conditions resembling those of the natural site of infection and that Sfp fimbriae may contribute to the adherence of the organisms to human intestinal epithelium.

Links

Publisher Full Text
ncbi.nlm.nih.gov
aem.asm.org
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Authors+Show Affiliations

Müsken A
Institute of Hygiene, University of Münster, Münster, Germany.
Bielaszewska M
No affiliation info available
Greune L
No affiliation info available
Schweppe CH
No affiliation info available
Müthing J
No affiliation info available
Schmidt H
No affiliation info available
Schmidt MA
No affiliation info available
Karch H
No affiliation info available
Zhang W
No affiliation info available

MeSH

AnaerobiosisCaco-2 CellsDNA PrimersEscherichia coli O157FermentationFimbriae ProteinsFimbriae, BacterialGene Expression Regulation, BacterialHemagglutination TestsHumansIntestinal MucosaMicroscopy, ElectronReverse Transcriptase Polymerase Chain ReactionSorbitolSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18083855

Citation

Müsken, Anne, et al. "Anaerobic Conditions Promote Expression of Sfp Fimbriae and Adherence of Sorbitol-fermenting Enterohemorrhagic Escherichia Coli O157:NM to Human Intestinal Epithelial Cells." Applied and Environmental Microbiology, vol. 74, no. 4, 2008, pp. 1087-93.
Müsken A, Bielaszewska M, Greune L, et al. Anaerobic conditions promote expression of Sfp fimbriae and adherence of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM to human intestinal epithelial cells. Appl Environ Microbiol. 2008;74(4):1087-93.
Müsken, A., Bielaszewska, M., Greune, L., Schweppe, C. H., Müthing, J., Schmidt, H., Schmidt, M. A., Karch, H., & Zhang, W. (2008). Anaerobic conditions promote expression of Sfp fimbriae and adherence of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM to human intestinal epithelial cells. Applied and Environmental Microbiology, 74(4), 1087-93.
Müsken A, et al. Anaerobic Conditions Promote Expression of Sfp Fimbriae and Adherence of Sorbitol-fermenting Enterohemorrhagic Escherichia Coli O157:NM to Human Intestinal Epithelial Cells. Appl Environ Microbiol. 2008;74(4):1087-93. PubMed PMID: 18083855.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anaerobic conditions promote expression of Sfp fimbriae and adherence of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM to human intestinal epithelial cells. AU - Müsken,Anne, AU - Bielaszewska,Martina, AU - Greune,Lilo, AU - Schweppe,Christian H, AU - Müthing,Johannes, AU - Schmidt,Herbert, AU - Schmidt,M Alexander, AU - Karch,Helge, AU - Zhang,Wenlan, Y1 - 2007/12/14/ PY - 2007/12/18/pubmed PY - 2008/3/19/medline PY - 2007/12/18/entrez SP - 1087 EP - 93 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 74 IS - 4 N2 - The sfp gene cluster, unique to sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:NM strains, encodes fimbriae that mediate mannose-resistant hemagglutination in laboratory E. coli strains but are not expressed in wild-type SF EHEC O157:NM strains under standard laboratory conditions. We investigated whether Sfp fimbriae are expressed under conditions that mimic the intestinal environment and whether they contribute to the adherence of SF EHEC O157:NM strains to human intestinal epithelial cells. The transcription of sfpA (encoding the major fimbrial subunit) was upregulated in all strains investigated, and all expressed SfpA and possessed fimbriae that reacted with an anti-SfpA antibody when the strains were grown on solid media under anaerobic conditions. Sfp expression was absent under aerobic conditions and in liquid media. Sfp upregulation under anaerobic conditions was significantly higher on blood agar and a medium simulating the colonic environment than on a medium simulating the ileal environment (P < 0.05). The induction of Sfp fimbriae in SF E. coli O157:NM strains correlates with increased adherence to Caco-2 and HCT-8 cells. Our data indicate that the expression of Sfp fimbriae in SF E. coli O157:NM strains is induced under conditions resembling those of the natural site of infection and that Sfp fimbriae may contribute to the adherence of the organisms to human intestinal epithelium. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/18083855/Anaerobic_conditions_promote_expression_of_Sfp_fimbriae_and_adherence_of_sorbitol_fermenting_enterohemorrhagic_Escherichia_coli_O157:NM_to_human_intestinal_epithelial_cells_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&amp;pmid=18083855 DB - PRIME DP - Unbound Medicine ER -