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[Potential vectors for molecular cloning in Brevibacterium flavum].

Abstract

Construction of the shuttle cloning vectors for Escherichia coli-Brevibacterium flavum system is described. Expression of the Sp/Sm resistance determinant derived from the Corynebacterium plasmid pCG4 was registered in Escherichia coli cells. The genetic determinant for Sp/Sm resistance was shown to be located in a 2.2 kb PstI-SphI fragment by the deletion analysis mapping in Escherichia coli cells. Using Escherichia coli as a host we cloned the unique 0.8 kb EcoRI-EcoRI fragment of Brevibacterium flavum bacteriophage phi BSh6 in the plasmids with dual replication origins. Blocking of the shuttle vector transfer to Brevibacterium flavum by the insertion of bacteriophage phi BSh6 DNA was observed. The deletion of entire phage fragment or a specific part of it made it possible introduction of plasmids harboured by Escherichia coli cells into Brevibacterium flavum. A potential vector for homologous DNA cloning in Brevibacterium flavum was constructed.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

rus

PubMed ID

1808511

Citation

Tolmachev, O E., and M M. Gusiatiner. "[Potential Vectors for Molecular Cloning in Brevibacterium Flavum]." Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, 1991, pp. 3-8.
Tolmachev OE, Gusiatiner MM. [Potential vectors for molecular cloning in Brevibacterium flavum]. Mol Gen Mikrobiol Virusol. 1991.
Tolmachev, O. E., & Gusiatiner, M. M. (1991). [Potential vectors for molecular cloning in Brevibacterium flavum]. Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, (11), 3-8.
Tolmachev OE, Gusiatiner MM. [Potential Vectors for Molecular Cloning in Brevibacterium Flavum]. Mol Gen Mikrobiol Virusol. 1991;(11)3-8. PubMed PMID: 1808511.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Potential vectors for molecular cloning in Brevibacterium flavum]. AU - Tolmachev,O E, AU - Gusiatiner,M M, PY - 1991/11/1/pubmed PY - 1991/11/1/medline PY - 1991/11/1/entrez SP - 3 EP - 8 JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia JO - Mol Gen Mikrobiol Virusol IS - 11 N2 - Construction of the shuttle cloning vectors for Escherichia coli-Brevibacterium flavum system is described. Expression of the Sp/Sm resistance determinant derived from the Corynebacterium plasmid pCG4 was registered in Escherichia coli cells. The genetic determinant for Sp/Sm resistance was shown to be located in a 2.2 kb PstI-SphI fragment by the deletion analysis mapping in Escherichia coli cells. Using Escherichia coli as a host we cloned the unique 0.8 kb EcoRI-EcoRI fragment of Brevibacterium flavum bacteriophage phi BSh6 in the plasmids with dual replication origins. Blocking of the shuttle vector transfer to Brevibacterium flavum by the insertion of bacteriophage phi BSh6 DNA was observed. The deletion of entire phage fragment or a specific part of it made it possible introduction of plasmids harboured by Escherichia coli cells into Brevibacterium flavum. A potential vector for homologous DNA cloning in Brevibacterium flavum was constructed. SN - 0208-0613 UR - https://www.unboundmedicine.com/medline/citation/1808511/[Potential_vectors_for_molecular_cloning_in_Brevibacterium_flavum]_ L2 - https://antibodies.cancer.gov/detail/CPTC-GPI-1 DB - PRIME DP - Unbound Medicine ER -