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AdeIJK, a resistance-nodulation-cell division pump effluxing multiple antibiotics in Acinetobacter baumannii.
Antimicrob Agents Chemother. 2008 Feb; 52(2):557-62.AA

Abstract

We have identified a second resistance-nodulation-cell division (RND)-type efflux pump, AdeIJK, in clinical isolate Acinetobacter baumannii BM4454. The adeI, adeJ, and adeK genes encode, respectively, the membrane fusion, RND, and outer membrane components of the pump. AdeJ belongs to the AcrB protein family (57% identity with AcrB from Escherichia coli). mRNA analysis by Northern blotting and reverse transcription-PCR indicated that the genes were cotranscribed. Overexpression of the cloned adeIJK operon was toxic in both E. coli and Acinetobacter. The adeIJK genes were detected in all of the 60 strains of A. baumannii tested. The two latter observations suggest that the AdeIJK complex might contribute to intrinsic but not to acquired antibiotic resistance in Acinetobacter. To characterize the substrate specificity of the pump, we have constructed derivatives of BM4454 in which adeIJK (strain BM4579), adeABC (strain BM4561), or both groups of genes (strain BM4652) were inactivated by deletion-insertion. Determination of the antibiotic susceptibility of these strains and of BM4652 and BM4579, in which the adeIJK operon was provided in trans, indicated that the AdeIJK pump contributes to resistance to beta-lactams, chloramphenicol, tetracycline, erythromycin, lincosamides, fluoroquinolones, fusidic acid, novobiocin, rifampin, trimethoprim, acridine, safranin, pyronine, and sodium dodecyl sulfate. The chemical structure of these molecules suggests that amphiphilic compounds are the preferred substrates. The AdeABC and AdeIJK efflux systems contributed in a more than additive fashion to tigecycline resistance.

Authors+Show Affiliations

Unité des Agents Antibactériens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18086852

Citation

Damier-Piolle, Laurence, et al. "AdeIJK, a Resistance-nodulation-cell Division Pump Effluxing Multiple Antibiotics in Acinetobacter Baumannii." Antimicrobial Agents and Chemotherapy, vol. 52, no. 2, 2008, pp. 557-62.
Damier-Piolle L, Magnet S, Brémont S, et al. AdeIJK, a resistance-nodulation-cell division pump effluxing multiple antibiotics in Acinetobacter baumannii. Antimicrob Agents Chemother. 2008;52(2):557-62.
Damier-Piolle, L., Magnet, S., Brémont, S., Lambert, T., & Courvalin, P. (2008). AdeIJK, a resistance-nodulation-cell division pump effluxing multiple antibiotics in Acinetobacter baumannii. Antimicrobial Agents and Chemotherapy, 52(2), 557-62.
Damier-Piolle L, et al. AdeIJK, a Resistance-nodulation-cell Division Pump Effluxing Multiple Antibiotics in Acinetobacter Baumannii. Antimicrob Agents Chemother. 2008;52(2):557-62. PubMed PMID: 18086852.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - AdeIJK, a resistance-nodulation-cell division pump effluxing multiple antibiotics in Acinetobacter baumannii. AU - Damier-Piolle,Laurence, AU - Magnet,Sophie, AU - Brémont,Sylvie, AU - Lambert,Thierry, AU - Courvalin,Patrice, Y1 - 2007/12/17/ PY - 2007/12/19/pubmed PY - 2008/4/30/medline PY - 2007/12/19/entrez SP - 557 EP - 62 JF - Antimicrobial agents and chemotherapy JO - Antimicrob. Agents Chemother. VL - 52 IS - 2 N2 - We have identified a second resistance-nodulation-cell division (RND)-type efflux pump, AdeIJK, in clinical isolate Acinetobacter baumannii BM4454. The adeI, adeJ, and adeK genes encode, respectively, the membrane fusion, RND, and outer membrane components of the pump. AdeJ belongs to the AcrB protein family (57% identity with AcrB from Escherichia coli). mRNA analysis by Northern blotting and reverse transcription-PCR indicated that the genes were cotranscribed. Overexpression of the cloned adeIJK operon was toxic in both E. coli and Acinetobacter. The adeIJK genes were detected in all of the 60 strains of A. baumannii tested. The two latter observations suggest that the AdeIJK complex might contribute to intrinsic but not to acquired antibiotic resistance in Acinetobacter. To characterize the substrate specificity of the pump, we have constructed derivatives of BM4454 in which adeIJK (strain BM4579), adeABC (strain BM4561), or both groups of genes (strain BM4652) were inactivated by deletion-insertion. Determination of the antibiotic susceptibility of these strains and of BM4652 and BM4579, in which the adeIJK operon was provided in trans, indicated that the AdeIJK pump contributes to resistance to beta-lactams, chloramphenicol, tetracycline, erythromycin, lincosamides, fluoroquinolones, fusidic acid, novobiocin, rifampin, trimethoprim, acridine, safranin, pyronine, and sodium dodecyl sulfate. The chemical structure of these molecules suggests that amphiphilic compounds are the preferred substrates. The AdeABC and AdeIJK efflux systems contributed in a more than additive fashion to tigecycline resistance. SN - 0066-4804 UR - https://www.unboundmedicine.com/medline/citation/18086852/AdeIJK_a_resistance_nodulation_cell_division_pump_effluxing_multiple_antibiotics_in_Acinetobacter_baumannii_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&pmid=18086852 DB - PRIME DP - Unbound Medicine ER -