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DNA polymerase delta is preferentially recruited during homologous recombination to promote heteroduplex DNA extension.
Mol Cell Biol. 2008 Feb; 28(4):1373-82.MC

Abstract

DNA polymerases play a central role during homologous recombination (HR), but the identity of the enzyme(s) implicated remains elusive. The pol3-ct allele of the gene encoding the catalytic subunit of DNA polymerase delta (Poldelta) has highlighted a role for this polymerase in meiotic HR. We now address the ubiquitous role of Poldelta during HR in somatic cells. We find that pol3-ct affects gene conversion tract length during mitotic recombination whether the event is initiated by single-strand gaps following UV irradiation or by site-specific double-strand breaks. We show that the pol3-ct effects on gene conversion are completely independent of mismatch repair, indicating that shorter gene conversion tracts in pol3-ct correspond to shorter extensions of primed DNA synthesis. Interestingly, we find that shorter repair tracts do not favor synthesis-dependent strand annealing at the expense of double-strand-break repair. Finally, we show that the DNA polymerases that have been previously suspected to mediate HR repair synthesis (Polepsilon and Poleta) do not affect gene conversion during induced HR, including in the pol3-ct background. Our results argue strongly for the preferential recruitment of Poldelta during HR.

Authors+Show Affiliations

CEA, DSV, iRCM, SIGRR, LERA, Fontenay-aux-Roses F-92265, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18086882

Citation

Maloisel, Laurent, et al. "DNA Polymerase Delta Is Preferentially Recruited During Homologous Recombination to Promote Heteroduplex DNA Extension." Molecular and Cellular Biology, vol. 28, no. 4, 2008, pp. 1373-82.
Maloisel L, Fabre F, Gangloff S. DNA polymerase delta is preferentially recruited during homologous recombination to promote heteroduplex DNA extension. Mol Cell Biol. 2008;28(4):1373-82.
Maloisel, L., Fabre, F., & Gangloff, S. (2008). DNA polymerase delta is preferentially recruited during homologous recombination to promote heteroduplex DNA extension. Molecular and Cellular Biology, 28(4), 1373-82.
Maloisel L, Fabre F, Gangloff S. DNA Polymerase Delta Is Preferentially Recruited During Homologous Recombination to Promote Heteroduplex DNA Extension. Mol Cell Biol. 2008;28(4):1373-82. PubMed PMID: 18086882.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA polymerase delta is preferentially recruited during homologous recombination to promote heteroduplex DNA extension. AU - Maloisel,Laurent, AU - Fabre,Francis, AU - Gangloff,Serge, Y1 - 2007/12/17/ PY - 2007/12/19/pubmed PY - 2008/2/20/medline PY - 2007/12/19/entrez SP - 1373 EP - 82 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 28 IS - 4 N2 - DNA polymerases play a central role during homologous recombination (HR), but the identity of the enzyme(s) implicated remains elusive. The pol3-ct allele of the gene encoding the catalytic subunit of DNA polymerase delta (Poldelta) has highlighted a role for this polymerase in meiotic HR. We now address the ubiquitous role of Poldelta during HR in somatic cells. We find that pol3-ct affects gene conversion tract length during mitotic recombination whether the event is initiated by single-strand gaps following UV irradiation or by site-specific double-strand breaks. We show that the pol3-ct effects on gene conversion are completely independent of mismatch repair, indicating that shorter gene conversion tracts in pol3-ct correspond to shorter extensions of primed DNA synthesis. Interestingly, we find that shorter repair tracts do not favor synthesis-dependent strand annealing at the expense of double-strand-break repair. Finally, we show that the DNA polymerases that have been previously suspected to mediate HR repair synthesis (Polepsilon and Poleta) do not affect gene conversion during induced HR, including in the pol3-ct background. Our results argue strongly for the preferential recruitment of Poldelta during HR. SN - 1098-5549 UR - https://www.unboundmedicine.com/medline/citation/18086882/DNA_polymerase_delta_is_preferentially_recruited_during_homologous_recombination_to_promote_heteroduplex_DNA_extension_ L2 - https://journals.asm.org/doi/10.1128/MCB.01651-07?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -