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Effect of folate, vitamin B6, and vitamin B12 intake and MTHFR C677T polymorphism on homocysteine concentrations of renal transplant recipients.

Abstract

Plasma hyperhomocysteinemia (HHcy) is considered a risk factor for chronic allograft dysfunction (CAD), the main cause of functional loss in transplant recipients. Genetic polymorphisms that alter enzymes involved in homocysteine (Hcy) metabolism, such as methylenetetrahydrofolate reductase (MTHFR), and vitamin deficiency can result in HHcy. The objectives of this study were to investigate the relationship between HHcy and CAD development, and to evaluate the effect of intake of folate and vitamins B6 and B12 as well as MTHFR C677T polymorphism on Hcy concentrations. Ninety-eight renal transplant recipients including 48 showing CAD and 50 with normal renal function (NRF), were included in this cross-sectional study. Peripheral blood samples were collected for plasma Hcy quantification by liquid chromatography/sequential mass spectrometry (LC-MS/MS), and for MTHFR polymorphism analysis using polymerase chain reaction-restriction fragment length polymorphism. Dietary intake was evaluated using a nutritional questionnaire. HHcy (P=.002) and higher mean concentrations of Hcy (P=.029) were associated with CAD. An association was observed between HHcy and 677T variant allele in the CAD group (P=.0005). There was no correlation between Hcy concentration and folate, vitamin B6 or vitamin B12 intake in the CAD group. However, a negative correlation was observed between Hcy concentration and folate intake (P=.043), and also between Hcy concentration and vitamin B6 intake (P=.030) in the NRF group. According to our study, HHcy is associated with CAD development. In patients with CAD, MTHFR polymorphism seems to have a greater effect on the Hcy concentration than the vitamin intake. Increased folate and vitamin B6 intakes seem to reduce Hcy concentrations among transplant recipients with NRF, and could contribute to reducing the risk of CAD development.

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  • Authors+Show Affiliations

    ,

    Research Unit in Genetics and Molecular Biology (UPGEM) São José do Rio Preto Medical School (FAMERP), SP, Brazil.

    , , , , , , , , ,

    Source

    Transplantation proceedings 39:10 2007 Dec pg 3163-5

    MeSH

    Cross-Sectional Studies
    Folic Acid
    Homocysteine
    Humans
    Hyperhomocysteinemia
    Kidney Function Tests
    Kidney Transplantation
    Methylenetetrahydrofolate Reductase (NADPH2)
    Polymorphism, Single Nucleotide
    Postoperative Complications
    Vitamin B 12
    Vitamin B 6

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    18089344

    Citation

    Biselli, P M., et al. "Effect of Folate, Vitamin B6, and Vitamin B12 Intake and MTHFR C677T Polymorphism On Homocysteine Concentrations of Renal Transplant Recipients." Transplantation Proceedings, vol. 39, no. 10, 2007, pp. 3163-5.
    Biselli PM, Sanches de Alvarenga MP, Abbud-Filho M, et al. Effect of folate, vitamin B6, and vitamin B12 intake and MTHFR C677T polymorphism on homocysteine concentrations of renal transplant recipients. Transplant Proc. 2007;39(10):3163-5.
    Biselli, P. M., Sanches de Alvarenga, M. P., Abbud-Filho, M., Ferreira-Baptista, M. A., Galbiatti, A. L., Goto, M. T., ... Pavarino-Bertelli, E. C. (2007). Effect of folate, vitamin B6, and vitamin B12 intake and MTHFR C677T polymorphism on homocysteine concentrations of renal transplant recipients. Transplantation Proceedings, 39(10), pp. 3163-5.
    Biselli PM, et al. Effect of Folate, Vitamin B6, and Vitamin B12 Intake and MTHFR C677T Polymorphism On Homocysteine Concentrations of Renal Transplant Recipients. Transplant Proc. 2007;39(10):3163-5. PubMed PMID: 18089344.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effect of folate, vitamin B6, and vitamin B12 intake and MTHFR C677T polymorphism on homocysteine concentrations of renal transplant recipients. AU - Biselli,P M, AU - Sanches de Alvarenga,M P, AU - Abbud-Filho,M, AU - Ferreira-Baptista,M A S, AU - Galbiatti,A L S, AU - Goto,M T Y, AU - Cardoso,M A, AU - Eberlin,M N, AU - Haddad,R, AU - Goloni-Bertollo,E M, AU - Pavarino-Bertelli,E C, PY - 2006/12/03/received PY - 2007/06/15/revised PY - 2007/08/08/accepted PY - 2007/12/20/pubmed PY - 2008/2/21/medline PY - 2007/12/20/entrez SP - 3163 EP - 5 JF - Transplantation proceedings JO - Transplant. Proc. VL - 39 IS - 10 N2 - Plasma hyperhomocysteinemia (HHcy) is considered a risk factor for chronic allograft dysfunction (CAD), the main cause of functional loss in transplant recipients. Genetic polymorphisms that alter enzymes involved in homocysteine (Hcy) metabolism, such as methylenetetrahydrofolate reductase (MTHFR), and vitamin deficiency can result in HHcy. The objectives of this study were to investigate the relationship between HHcy and CAD development, and to evaluate the effect of intake of folate and vitamins B6 and B12 as well as MTHFR C677T polymorphism on Hcy concentrations. Ninety-eight renal transplant recipients including 48 showing CAD and 50 with normal renal function (NRF), were included in this cross-sectional study. Peripheral blood samples were collected for plasma Hcy quantification by liquid chromatography/sequential mass spectrometry (LC-MS/MS), and for MTHFR polymorphism analysis using polymerase chain reaction-restriction fragment length polymorphism. Dietary intake was evaluated using a nutritional questionnaire. HHcy (P=.002) and higher mean concentrations of Hcy (P=.029) were associated with CAD. An association was observed between HHcy and 677T variant allele in the CAD group (P=.0005). There was no correlation between Hcy concentration and folate, vitamin B6 or vitamin B12 intake in the CAD group. However, a negative correlation was observed between Hcy concentration and folate intake (P=.043), and also between Hcy concentration and vitamin B6 intake (P=.030) in the NRF group. According to our study, HHcy is associated with CAD development. In patients with CAD, MTHFR polymorphism seems to have a greater effect on the Hcy concentration than the vitamin intake. Increased folate and vitamin B6 intakes seem to reduce Hcy concentrations among transplant recipients with NRF, and could contribute to reducing the risk of CAD development. SN - 0041-1345 UR - https://www.unboundmedicine.com/medline/citation/18089344/Effect_of_folate_vitamin_B6_and_vitamin_B12_intake_and_MTHFR_C677T_polymorphism_on_homocysteine_concentrations_of_renal_transplant_recipients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-1345(07)01126-8 DB - PRIME DP - Unbound Medicine ER -