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Characterization of true-branching cyanobacteria from geothermal sites and hot springs of Costa Rica.
Environ Microbiol. 2008 Feb; 10(2):460-73.EM

Abstract

Costa Rica is at the centre of the Mesoamerican biodiversity hotspot. Little is known about cyanobacteria from this region so far. Here, four isolates of the order Stigonematales (section V) were characterized in a polyphasic approach. All strains were isolated from geothermal sites and hot springs of Costa Rica. However, one of them, identified as Westiellopsis sp. Ar73, did not grow at more than 40 degrees C. Based on its identical 16S rRNA to several previously isolated Westiellopsis sp. and Fischerella muscicola strains, a ubiquitous distribution throughout tropical and subtropical regions can be implied. In contrast, the isolates MV9, MV11 and RV14 grew well up to 50-55 degrees C. Based on morphologic, ultrastructural, molecular and physiologic data, MV9, MV11 and RV14 were identified to belong to the genus Fischerella. Two distinct intergenic transcribed spacer (ITS) types, with or without tRNA genes, were detected for all Stigonematales analysed here, indicating ITS polymorphism as a characteristic feature of heterocystous cyanobacteria. In phylogenetic trees, these Fischerella spp. formed a new and distinct clade within the wider lineage of thermophilic Fischerella (Mastigocladus cf. laminosus), which might represent a geographic lineage. Thus, geographic isolation may be an underestimated aspect of microbial evolution. The strains presented here are suitable as new models to study this group of cyanobacteria.

Authors+Show Affiliations

University of Freiburg, Faculty of Biology, Schänzlestr. 1, D-79104 Freiburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18093164

Citation

Finsinger, Karin, et al. "Characterization of True-branching Cyanobacteria From Geothermal Sites and Hot Springs of Costa Rica." Environmental Microbiology, vol. 10, no. 2, 2008, pp. 460-73.
Finsinger K, Scholz I, Serrano A, et al. Characterization of true-branching cyanobacteria from geothermal sites and hot springs of Costa Rica. Environ Microbiol. 2008;10(2):460-73.
Finsinger, K., Scholz, I., Serrano, A., Morales, S., Uribe-Lorio, L., Mora, M., Sittenfeld, A., Weckesser, J., & Hess, W. R. (2008). Characterization of true-branching cyanobacteria from geothermal sites and hot springs of Costa Rica. Environmental Microbiology, 10(2), 460-73.
Finsinger K, et al. Characterization of True-branching Cyanobacteria From Geothermal Sites and Hot Springs of Costa Rica. Environ Microbiol. 2008;10(2):460-73. PubMed PMID: 18093164.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of true-branching cyanobacteria from geothermal sites and hot springs of Costa Rica. AU - Finsinger,Karin, AU - Scholz,Ingeborg, AU - Serrano,Aurelio, AU - Morales,Saylen, AU - Uribe-Lorio,Lorena, AU - Mora,Marielos, AU - Sittenfeld,Ana, AU - Weckesser,Jürgen, AU - Hess,Wolfgang R, Y1 - 2007/12/17/ PY - 2007/12/21/pubmed PY - 2008/2/27/medline PY - 2007/12/21/entrez SP - 460 EP - 73 JF - Environmental microbiology JO - Environ Microbiol VL - 10 IS - 2 N2 - Costa Rica is at the centre of the Mesoamerican biodiversity hotspot. Little is known about cyanobacteria from this region so far. Here, four isolates of the order Stigonematales (section V) were characterized in a polyphasic approach. All strains were isolated from geothermal sites and hot springs of Costa Rica. However, one of them, identified as Westiellopsis sp. Ar73, did not grow at more than 40 degrees C. Based on its identical 16S rRNA to several previously isolated Westiellopsis sp. and Fischerella muscicola strains, a ubiquitous distribution throughout tropical and subtropical regions can be implied. In contrast, the isolates MV9, MV11 and RV14 grew well up to 50-55 degrees C. Based on morphologic, ultrastructural, molecular and physiologic data, MV9, MV11 and RV14 were identified to belong to the genus Fischerella. Two distinct intergenic transcribed spacer (ITS) types, with or without tRNA genes, were detected for all Stigonematales analysed here, indicating ITS polymorphism as a characteristic feature of heterocystous cyanobacteria. In phylogenetic trees, these Fischerella spp. formed a new and distinct clade within the wider lineage of thermophilic Fischerella (Mastigocladus cf. laminosus), which might represent a geographic lineage. Thus, geographic isolation may be an underestimated aspect of microbial evolution. The strains presented here are suitable as new models to study this group of cyanobacteria. SN - 1462-2920 UR - https://www.unboundmedicine.com/medline/citation/18093164/Characterization_of_true_branching_cyanobacteria_from_geothermal_sites_and_hot_springs_of_Costa_Rica_ DB - PRIME DP - Unbound Medicine ER -