Renoprotective action of L-carnitine in fructose-induced metabolic syndrome.Diabetes Obes Metab. 2008 Feb; 10(2):171-80.DO
Rats fed high dosage of fructose that form a well-known experimental model of the metabolic syndrome also display progressive renal disturbances. The present study evaluates the influence of l-carnitine (CA) administration on oxidant-antioxidant balance, protein damage and lipid levels in kidney of rats administered high dose of fructose.
Adult male Wistar rats were divided into four groups of 10 rats each. Groups I and IV animals received starch-based control diet, while groups II and III rats were fed a high-fructose diet (60 g/100 g). Groups III and IV animals additionally received CA (300 mg/kg/day) for 60 days. The extent of lipid peroxidation, enzymatic and non-enzymatic antioxidants and lipid levels were measured after 60 days. The accumulation of nitrated and oxidatively modified proteins in kidney was also measured by immunohistochemical study with specific antibodies.
Fructose-fed rats exhibited increased levels of peroxidation end products, diminished antioxidant status, increased staining for the presence of 4-hydroxy-2-nonenal, 2,4-dinitrophenol and 3-nitrotyrosine protein adducts and lipid accumulation in kidney. CA administration attenuated these pathological renal alterations.
The benefits of CA in this model suggest the therapeutic use of CA to counter the kidney changes associated with metabolic syndrome.