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Identification of genes differentially expressed by prematurely fused human sutures using a novel in vivo - in vitro approach.
Differentiation 2008; 76(5):531-45D

Abstract

Craniosynostosis is the premature fusion of calvarial sutures. It results from abnormal differentiation or proliferation of cells within the osteogenic fronts of growing calvarial bones. To date, research has focused on animal models and in vitro organ and tissue culture to determine the molecular mechanisms controlling calvarial suture morphogenesis. Here, we test a new, in vivo-in vitro approach based on the hypothesis that calvarial suture cells passaged in minimal medium exhibit a stable gene expression profile similar to undifferentiated osteoblastic cells that can provide a benchmark for comparison with in vivo expression of differentiated tissue. We show that tissue-specific expression is lost after the first passage and, using cDNA microarrays, compare expression between fused suture tissue from craniosynostosis patients and in vitro de-differentiated explant cells. A large number of differentially expressed genes were identified, including novel genes WIF1, LEF1, SATB2, RARRES1, DEFA1, DMP1, PTPRZ1, and PTPRC, as well as those commonly associated with human suture morphogenesis, e.g., FGF2, MSX2, and BMP2. Two differentially expressed genes, WIF1 and FGF2, were further examined in an in vivo-in vivo comparison between unfused and prematurely fused tissue. The same pattern of differential expression was observed in each case, further validating the ability of our in vivo-in vitro approach to identify genes involved in in vivo human calvarial tissue differentiation.

Authors+Show Affiliations

Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD, Australia. anna.coussens@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18093228

Citation

Coussens, Anna K., et al. "Identification of Genes Differentially Expressed By Prematurely Fused Human Sutures Using a Novel in Vivo - in Vitro Approach." Differentiation; Research in Biological Diversity, vol. 76, no. 5, 2008, pp. 531-45.
Coussens AK, Hughes IP, Wilkinson CR, et al. Identification of genes differentially expressed by prematurely fused human sutures using a novel in vivo - in vitro approach. Differentiation. 2008;76(5):531-45.
Coussens, A. K., Hughes, I. P., Wilkinson, C. R., Morris, C. P., Anderson, P. J., Powell, B. C., & van Daal, A. (2008). Identification of genes differentially expressed by prematurely fused human sutures using a novel in vivo - in vitro approach. Differentiation; Research in Biological Diversity, 76(5), pp. 531-45.
Coussens AK, et al. Identification of Genes Differentially Expressed By Prematurely Fused Human Sutures Using a Novel in Vivo - in Vitro Approach. Differentiation. 2008;76(5):531-45. PubMed PMID: 18093228.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of genes differentially expressed by prematurely fused human sutures using a novel in vivo - in vitro approach. AU - Coussens,Anna K, AU - Hughes,Ian P, AU - Wilkinson,Christopher R, AU - Morris,C Phillip, AU - Anderson,Peter J, AU - Powell,Barry C, AU - van Daal,Angela, Y1 - 2007/12/17/ PY - 2007/12/21/pubmed PY - 2008/9/30/medline PY - 2007/12/21/entrez SP - 531 EP - 45 JF - Differentiation; research in biological diversity JO - Differentiation VL - 76 IS - 5 N2 - Craniosynostosis is the premature fusion of calvarial sutures. It results from abnormal differentiation or proliferation of cells within the osteogenic fronts of growing calvarial bones. To date, research has focused on animal models and in vitro organ and tissue culture to determine the molecular mechanisms controlling calvarial suture morphogenesis. Here, we test a new, in vivo-in vitro approach based on the hypothesis that calvarial suture cells passaged in minimal medium exhibit a stable gene expression profile similar to undifferentiated osteoblastic cells that can provide a benchmark for comparison with in vivo expression of differentiated tissue. We show that tissue-specific expression is lost after the first passage and, using cDNA microarrays, compare expression between fused suture tissue from craniosynostosis patients and in vitro de-differentiated explant cells. A large number of differentially expressed genes were identified, including novel genes WIF1, LEF1, SATB2, RARRES1, DEFA1, DMP1, PTPRZ1, and PTPRC, as well as those commonly associated with human suture morphogenesis, e.g., FGF2, MSX2, and BMP2. Two differentially expressed genes, WIF1 and FGF2, were further examined in an in vivo-in vivo comparison between unfused and prematurely fused tissue. The same pattern of differential expression was observed in each case, further validating the ability of our in vivo-in vitro approach to identify genes involved in in vivo human calvarial tissue differentiation. SN - 1432-0436 UR - https://www.unboundmedicine.com/medline/citation/18093228/Identification_of_genes_differentially_expressed_by_prematurely_fused_human_sutures_using_a_novel_in_vivo___in_vitro_approach_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0301-4681(09)60095-5 DB - PRIME DP - Unbound Medicine ER -