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[Analysis of positive results in mediace rapid plasma reagin and Treponema pallidum latex agglutination as the automated syphilis test].
Korean J Lab Med. 2007 Oct; 27(5):324-9.KJ

Abstract

BACKGROUND

We compared the results of automated and quantitative methods for the diagnosis of syphilis, Mediace Rapid Plasma Reagin (RPR) and Mediace Treponema pallidum Latex Agglutination (TPLA) (Sekisui Chemical Co., Ltd, Japan) with those of conventional methods.

METHODS

Sera from 3,896 persons who had health checkups between December 2005 and November 2006 were included in the evaluation of positive rates and biological false positives (BFP) for Mediace RPR and TPLA. In addition, 134 patients' sera positive for automated Mediace RPR or TPLA were tested for VDRL and TPHA. Discrepancies between TPLA and TPHA results were confirmed by the RecomBlot Treponemal IgG/IgM (Mikrogen GmbH, Germany). Automated Mediace RPR and TPLA were performed using the Hitachi 7600 chemistry autoanalyzer (Hitachi, Japan). Samples with positive Mediace RPR and negative TPLA results were defined as BFP.

RESULTS

Positive rate of automated Mediace RPR was 0.23% (9/3,896). BFP of the Mediace RPR was 0.18%. Positive rate of automated TPLA was 1.62% (37/2,284). Among the 134 patients' sera, 33 (24.6%) showed a discrepancy between conventional VDRL and automated Mediace RPR results: Among 31 Mediace RPR(+)/VDRL(-) sera, 13 were positive and 18 were negative for TPLA. The remaining 2 sera of discrepancy with Mediace RPR(-)/VDRL(+) were all positive for TPLA. There were seven sera that showed a discrepancy between automated TPLA and TPHA results: Two sera with Mediace RPR(+)/TPLA(-)/TPHA(+) showed negative recomBlot Treponemal IgG/IgM results, and among five sera with TPLA(+)/TPHA(-), three demonstrated IgG or IgM by recomBlot Treponemal IgG/IgM.

CONCLUSIONS

The results of comparison data demonstrated that automated TPLA results had a high concordance with recomBlot Treponemal IgG/IgM results. Moreover, there are additional advantages of automated methods such as quantitative detection, low infection risk, and no influence by human handling.

Authors+Show Affiliations

Department of Laboratory Medicine, College of Medicine, Dongguk University, Gyeonggi Province, Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

kor

PubMed ID

18094596

Citation

Huh, Hee Jin, et al. "[Analysis of Positive Results in Mediace Rapid Plasma Reagin and Treponema Pallidum Latex Agglutination as the Automated Syphilis Test]." The Korean Journal of Laboratory Medicine, vol. 27, no. 5, 2007, pp. 324-9.
Huh HJ, Lee KK, Kim ES, et al. [Analysis of positive results in mediace rapid plasma reagin and Treponema pallidum latex agglutination as the automated syphilis test]. Korean J Lab Med. 2007;27(5):324-9.
Huh, H. J., Lee, K. K., Kim, E. S., & Chae, S. L. (2007). [Analysis of positive results in mediace rapid plasma reagin and Treponema pallidum latex agglutination as the automated syphilis test]. The Korean Journal of Laboratory Medicine, 27(5), 324-9.
Huh HJ, et al. [Analysis of Positive Results in Mediace Rapid Plasma Reagin and Treponema Pallidum Latex Agglutination as the Automated Syphilis Test]. Korean J Lab Med. 2007;27(5):324-9. PubMed PMID: 18094596.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Analysis of positive results in mediace rapid plasma reagin and Treponema pallidum latex agglutination as the automated syphilis test]. AU - Huh,Hee Jin, AU - Lee,Kyo Kwan, AU - Kim,Eu Suk, AU - Chae,Seok-Lae, PY - 2007/12/21/pubmed PY - 2008/11/19/medline PY - 2007/12/21/entrez SP - 324 EP - 9 JF - The Korean journal of laboratory medicine JO - Korean J Lab Med VL - 27 IS - 5 N2 - BACKGROUND: We compared the results of automated and quantitative methods for the diagnosis of syphilis, Mediace Rapid Plasma Reagin (RPR) and Mediace Treponema pallidum Latex Agglutination (TPLA) (Sekisui Chemical Co., Ltd, Japan) with those of conventional methods. METHODS: Sera from 3,896 persons who had health checkups between December 2005 and November 2006 were included in the evaluation of positive rates and biological false positives (BFP) for Mediace RPR and TPLA. In addition, 134 patients' sera positive for automated Mediace RPR or TPLA were tested for VDRL and TPHA. Discrepancies between TPLA and TPHA results were confirmed by the RecomBlot Treponemal IgG/IgM (Mikrogen GmbH, Germany). Automated Mediace RPR and TPLA were performed using the Hitachi 7600 chemistry autoanalyzer (Hitachi, Japan). Samples with positive Mediace RPR and negative TPLA results were defined as BFP. RESULTS: Positive rate of automated Mediace RPR was 0.23% (9/3,896). BFP of the Mediace RPR was 0.18%. Positive rate of automated TPLA was 1.62% (37/2,284). Among the 134 patients' sera, 33 (24.6%) showed a discrepancy between conventional VDRL and automated Mediace RPR results: Among 31 Mediace RPR(+)/VDRL(-) sera, 13 were positive and 18 were negative for TPLA. The remaining 2 sera of discrepancy with Mediace RPR(-)/VDRL(+) were all positive for TPLA. There were seven sera that showed a discrepancy between automated TPLA and TPHA results: Two sera with Mediace RPR(+)/TPLA(-)/TPHA(+) showed negative recomBlot Treponemal IgG/IgM results, and among five sera with TPLA(+)/TPHA(-), three demonstrated IgG or IgM by recomBlot Treponemal IgG/IgM. CONCLUSIONS: The results of comparison data demonstrated that automated TPLA results had a high concordance with recomBlot Treponemal IgG/IgM results. Moreover, there are additional advantages of automated methods such as quantitative detection, low infection risk, and no influence by human handling. SN - 1598-6535 UR - https://www.unboundmedicine.com/medline/citation/18094596/[Analysis_of_positive_results_in_mediace_rapid_plasma_reagin_and_Treponema_pallidum_latex_agglutination_as_the_automated_syphilis_test]_ L2 - http://www.annlabmed.org/journal/viewJournal.html?year=2007&vol=27&page=324 DB - PRIME DP - Unbound Medicine ER -